價格詳細(xì)資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】注意事項

1. 當(dāng)混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY-L0310 氫溴酸東莨菪堿114-49-8      Scopolamine hydrobromide     114-49-8  20mg                          

QY-L0311 東莨菪苷531-44-2 Scopolin   531-44-2  20mg                          

QY-L0312 大薊苷28978-02-1 Pectolinarin      28978-02-1       20mg                          

QY-L0313 柳穿魚黃素520-12-7      Pectolinarigenin       520-12-7  20mg                          

QY-L0314 豆腐果苷80154-34-3      Helicid      80154-34-3       20mg                          

QY-L0315 滇烏頭70578-24-4 Yunaconitine；Guayewuanine B     70578-24-4       20mg                           【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

QY-L0316 (-)-丁香樹脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷136997-64-3         (-)-Syringaresnol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside       136997-64-3    20mg                          

QY-L0317 丁香樹脂醇雙葡萄糖苷         Syringaresinol-di-O-glucoside            20mg                          

QY-L0318 斷血流皂苷A 916347-31-4    Clinodiside A     916347-31-4    20mg                          

QY-L0319          Cannabisin A              20mg                          

QY-L0320 杜鵑素24211-30-1 Farrerol    24211-30-1       20mg                          

QY-L0321 正丁基苯酞6066-49-5   3-n-Butylphathlide   6066-49-5         20mg                          

QY-L0322 丁烯基苯酞551-08-6      n-Butylidenephthalide     551-08-6  20mg                          

QY-L0323 洋川芎內(nèi)酯A62006-39-7        Senkyunolide A         62006-39-7       20mg                          

QY-L0324 藁本內(nèi)酯4431-01-0        Ligustilide         4431-01-0         20mg                          

QY-L0325 大蒜素（UV98%)539-86-6      Allicin        539-86-6  20mg                          

QY-L0326 蒜氨酸556-27-4      (S)-3-(Allylsulphinyl)-L-alanine  556-27-4  20mg                          

QY-L0327 大戟二烯醇514-47-6      Euphol      514-47-6  20mg                          

QY-L0328 燈盞花甲素    apigenin-7-O-glucronide                20mg                          

QY-L0329 野黃芩苷27740-01-8      Scutellarin        27740-01-8       20mg                          

QY-L0330 高黃芩素529-53-3 Scutellartln       529-53-3  20mg                          

QY-L0331 德爾塔林6836-11-9      Deltaline  1803152  20mg                           【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

QY-L0332 麥冬皂苷A 11054-24-3  Ophiopogonin A        11054-24-3    20mg                          

QY-L0333 麥冬皂苷B 38971-41-4  Ophiopogonin B        38971-41-4    20mg                          

QY-L0334 麥冬皂苷C911819-08-4         Ophiopogonin C       911819-08-4   20mg                          

QY-L0335 麥冬皂苷D945619-74-9          Ophiopogonin D       945619-74-9    20mg                          

QY-L0336 短葶山麥冬皂苷C87480-46-4       Liriope muscari baily saponins C       87480-46-4     20mg                          

QY-L0337 山麥冬皂苷B Liriopesides B            20mg                          

QY-L0338 魯斯可皂苷元472-11-7 Ruscogenin       472-11-7  20mg                          

QY-L0339 多舌飛蓬苷     6’-O-Caffeoylerigeroside                 20mg                          

QY-L0340 20830-75-5       Digoxin     20830-75-5       20mg                          

QY-L0341 莪術(shù)醇4871-97-0   Curcumol 4871-97-0         20mg                          

QY-L0342 莪術(shù)烯   Curzerene                20mg                          

9020962  Notebook, small instruments            CNY                              【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

9020729  Cooling adapter, MTP, RB, v2, Qsym                 CNY                             

9020710  Fan, electrical, 80mm, C34               CNY                             

9020702  Table plate, complete, C12               CNY                             

9016654  Microplate Station 8000, tall            CNY                             

9016584  Plate Stacker, RCS1           CNY                             

9016521  Bucket  Set (2), for microplate, QNG              CNY                             

9016483  Adapter PCB, Gateway, Kloehn                 CNY                             

9016387  EZ1 DNA Investigator Card               CNY                             

9016308  TFT Monitor, 17"               CNY                             

9016172  Software QIAsoft M48              CNY                             

9019175  Lens array, PyroQ96MD            CNY                             

9011925  Holder, bottle 30ml          CNY                             

9011891  Tubing, teflon, M6, 3 x 0.5 mm, 0.1 m              CNY                             

9011872  Shaker, Tube Rack for Plato 3           CNY                             

9011860  Cooling/heating unit, BR8000 v1.01                  CNY                             

9236850  Elution Microplate Adapter 3000, SBS              CNY                              【人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA試劑盒現(xiàn)貨】

9019141  PCB 1 - Connection w. valves, PyroQ96ID                  CNY                             

9019130  Cable - Pressure, PyroQ96ID             CNY                             

9019118  Motor - X/Y, PyroQ96ID            CNY                             

9019073  PyroMark Plate Bin, PyroQ96MD             CNY                             

9022698  36- Well Rotor, Calibration Only                CNY                             

9019015  Cartridge rack, EZ1xl                 CNY                             

9018947  Adapter 100-Well Honeycomb-Well Plate                 CNY                             

9016049  X-Box, BR8 RD v1.02                  CNY                             

9015919  Valve, magnet, dispenser, BR8000 V2              CNY                             

9015884  Incubator Tower, RCS1              CNY                             

9015810  Precision Pipetting Upgrade Kit                CNY                             

9015555  Cable , Clamp Motor, highflex, BR8000RD                CNY                             

9019161  Gasket - Tips, PyroQ96MD                CNY                             

9019152  PCB - Instrument Control, PyroQ96MD            CNY