【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QKG-312              規(guī)格：500ml                                      

QKG-313       麗春紅染色試劑 Ponceau Stain Reagent     規(guī)格：100ml                             

QKG-314              規(guī)格：500ml                                      

QKG-315       PBS (pH7.5, 10×) 規(guī)格：500ml                                      

QKG-316       PBST (pH7.5, 10×)      規(guī)格：500ml                                      

QKG-317       TBS (pH8.0, 10×) 規(guī)格：500ml                                      

QKG-318       TBST (pH8.0, 10×)      規(guī)格：500ml                                       【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】

QKG-319       Tris-Glycine Transfer Buffer (pH8.3, 10×) 規(guī)格：500ml                                      

QKG-320       Western Blot Ladder Pen       規(guī)格：1000次                                 

QKG-321              規(guī)格：5000次                                     

QKG-322       NC膜 (0.45 μm) 規(guī)格：14cm×20cm/張                                     

QKG-323       Annexin V-FITC/PI 凋亡檢測試劑盒 規(guī)格：50次                             

QKG-324       細胞周期與凋亡檢測試劑盒       規(guī)格：50次                             

QKG-325       Annexin V-Alexa Fluor 488/PI 凋亡檢測試劑盒 規(guī)格：50次                             

QKG-326       Annexin V-Alexa Fluor 647/PI 凋亡檢測試劑盒 規(guī)格：50次                             

QKG-327       Annexin V-PE/7-AAD 凋亡檢測試劑盒      規(guī)格：50次                             

QY1101   Annexin V-FITC/PI細胞凋亡檢測試劑盒   20                               

QY1102   Annexin V-EGFP/PI細胞凋亡檢測試劑盒  10                               

QY-AB1103     線粒體膜電位檢測試劑盒(JC-1) 10                               

QY-AB1104     DNA Ladder檢測試劑盒      20                               

QY-AB1105     TUNEL細胞凋亡原位檢測試劑盒（顯色法，通用型）  20                               

QY-AB1106     TUNEL細胞凋亡原位檢測試劑盒（顯色法，石蠟切片）      20                               

QY-AB1107     TUNEL細胞凋亡原位檢測試劑盒（顯色法，細胞）      20                                【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】

QY-AB1108     TUNEL細胞凋亡原位檢測試劑盒（顯色法，冰凍切片）      20                               

9QY-AB1109   一步法TUNEL細胞凋亡原位檢測試劑盒（通用型）     20                               

QY-AB1110     TUNEL細胞凋亡原位檢測試劑盒（熒光及顯色法，石蠟切片）  20                               

QY-AB1111     TUNEL細胞凋亡原位檢測試劑盒（熒光及顯色法，細胞）  20                               

QY-AB1112     TUNEL細胞凋亡原位檢測試劑盒（熒光及顯色法，冰凍切片）  20                               

QY-AB1113     Caspase-3活性檢測試劑盒(分光光度法)   20                               

QY-AB1114     Caspase-8活性檢測試劑盒(分光光度法)   20                               

QY-AB1115     Caspase-9活性檢測試劑盒(分光光度法)   20                               

QY-AB1116     細胞周期檢測試劑盒    20                               

QY-AB1117     MTT細胞增殖及細胞毒性檢測試劑盒      500                             

133156    NeXtalStock BIS-TRIS Propane pH8.5 (100)             CNY                           

133151    NeXtalStock BIS-TRIS pH 6.5 (100)           CNY                           

133074    NeXtalStock PEG 1,000 (200)             CNY                           

133071    NeXtalStock Nickel(II) chloride (200)         CNY                           

870111    therascreen EGFR RGQ PCR Kit CE (24)           CNY                           

805923    REPLI-g Service,  Single Tube (100 µg)           CNY                           

9015356  Interlock switch 2, for maintenance            CNY                           

9015345  Board, PSB            CNY                           

133042    NeXtalStock DL-Malic Acid (200)        CNY                           

133037    NeXtalStock Dimethyl sulfoxide (200)         CNY                           

9018394  Key, User, red, QIAxcel FA          CNY                           

9018337  Tubing, Valve to injection needle (set)          CNY                           

9001669  Rotor-Gene Q 6plex (w/o PC) PrioPLUS     "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

9001668  Rotor-Gene Q 6plex (w/o PC) Priority  Rotor-Gene Q 6plex (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

951336    MagAttract DNA Blood Mini M48 Kit (192)  MagAttract Suspension B, buffers, ａnd RNase-free water for up to 192 x 200 ul preps   CNY                           

37582     Qproteome Nuclear Protein Kit            CNY                            【人凋亡信號調(diào)節(jié)激酶I(ASK-1)ELISA試劑盒廠家】

9001640  Rotor-Gene Q 5plex System         CNY                           

290013    mericon Salmonella spp Kit (24)           CNY                           

231124    QIAGEN PCR Cloning Kit (40)     For 40 reactions: 2x Ligation Master Mix (200 ul), pDrive Cloning Vector (2.0 ug), distilled water (1.7 ml)   CNY                           

133149    NeXtalStock BICINE pH 9.0 (200)              CNY                           

219610    miRNeasy Serum/Plasma Spike-In Control          CNY                           

9015319  Sensor, photo, EE-SX673A          CNY                           

133014    NeXtalStock Ammonium phosphate (200)           CNY                           

1045800  Ni-NTA Superflow Cartridge Kit (1ml)         CNY                           

950107    Reagent Pack, DX  The Reagent Pack, DX,  which is used together with the DNA Plasticware Pack, QIAxtractor (#950037) provides fully automated DNA purification on the QIAxtractor. The combination of reagents ａnd plasticware enables purification of genomic DNA from virtually all types of sample, with a throughput of 8-96 samples per run.  CNY                           

9018319  Fuses 8A, centrifuge board (110V), QIAC           CNY                           

9018305  EZ1 Advanced DSP DNA Blood Card           CNY                           

9015285  Filter, HEPA, M48         CNY                           

9018301  EZ1 Advanced DNA Bacteria Card        CNY                           

9018289  Belttool, Y-Drive, Adjust        CNY                           

800388    Manual Sequence Editing              CNY