價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】注意事項

1. 當(dāng)混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標(biāo)準曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QTR-1157         金黃色葡萄球菌檢測試劑盒（探針法，98T）                                                

QTR-1158         亞硫酸鹽檢測試劑盒                                                  

QTR-1159         杏仁過敏檢測試劑盒                                                  

QTR-1160         蛋過敏原檢測試劑盒                                                  

QTR-1161         麥膠蛋白過敏原檢測試劑盒                                                       

QTR-1162         牛奶過敏原檢測試劑盒                                                       

QTR-1163         花生過敏原檢測試劑盒                                                       

QTR-1164         榛子過敏原檢測試劑盒                                                       

QTR-1165         大豆過敏原檢測試劑盒                                                        【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

QTR-1166         過敏原提取試劑盒（20T）                                                

QTR-1167         克倫特羅檢測試劑盒(96孔)                                               

QTR-1168         農(nóng)藥殘留檢測試劑盒（25T）                                                     

QTR-1169         豬鏈球菌2型熒光PCR檢測試劑盒     48                                         

QTR-1170         豬繁殖與呼吸綜合征病毒美洲型熒光RT-PCR檢測試劑盒        48                                         

QTR-1171         戊型肝炎病毒熒光RT-PCR檢測試劑盒        48                                         

QTR-1172         鯉春病熒光RT-PCR檢測試劑盒   48                                         

QTR-1173         狂犬病病毒熒光RT-PCR檢測試劑盒   48                                         

QTR-1174         傳染性造血器官壞死病病毒RT-PCR檢測試劑盒        48                                         

QTR-1175         H3N8馬流感病毒熒光RT-PCR檢測試劑盒 48                                         

QTR-1176         禽流感病毒和新城疫病毒熒光RT-PCR檢測試劑盒   48                                         

QTR-1177         禽滑液囊支原體抗體診斷試劑盒                                                       

QTR-1178         禽滑液囊支原體抗體診斷試劑盒                                                       

QTR-1179         雞新城疫病毒抗體檢測試劑盒                                                  

QTR-1180         鼠肝炎病毒抗體檢測試劑盒                                                       

QTR-1181         禽肺病毒（禽氣管炎）抗體檢測試劑盒                                                   【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

QTR-1182         羊邊界病毒抗體診斷試劑盒                                                       

QTR-1183         沙門氏菌共價混合物酶聯(lián)法抗體診斷試劑盒                                                  

QTR-1184         豬偽狂犬病毒gB抗體診斷試劑盒                                                     

QTR-1185         豬偽狂犬病毒gE抗體診斷試劑盒（gE抗體）                                               

QTR-1186         口蹄疫病毒抗體診斷試劑盒(3ABC抗體)                                                   

QTR-1187         牛布魯氏桿菌競爭酶聯(lián)法抗體診斷試劑盒                                                       

QTR-1188         牛布魯氏桿菌間接酶聯(lián)法抗體診斷試劑盒                                                       

QTR-1189         牛副流感3型抗體診斷試劑盒                                                   

9236148  Locator Plate, 384 well, BR Rapid Plate           CNY                             

9230679  Rack for reagents, Trough IBG                  CNY                             

9021123  Valve Manifold, Upper, DCU             CNY                             

9021102  Skin Panel, Lower Left Side, DCU              CNY                             

9021049  Set, transparent panel, Qsym           CNY                             

9021026  Set, cables, transfer module, Qsym                   CNY                             

9021012  Controller, Z Axis, VR, DCU                CNY                             

9020993  Pipettor Assembly, DCU            CNY                             

9020907  Cover, HEPA filter sys, SMC, QGX              CNY                             

9020905  UV light assembly, SMC, QGX           CNY                             

9020780  Notebook 1 XP          CNY                             

9020701  Clamp, table, single sided, C/S, C42                   CNY                             

9020688  Cover - Bottom, PyroQ24                   CNY                             

9020682  Pinch valve, PV02, QXT             CNY                              【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

9020640  Baseboard, front, AS, Cabinet Qsym                 CNY                             

9020639  Baseboard, front, SP, Cabinet Qsym                  CNY                             

9020365  Optical sensor, AS, Qsym          CNY                             

9020336  Pipetting head, 1 ch. (EXCHANGE), Cx2            CNY                             

9020315  PM kit, C12 & QIAgility (UV/HEPA), CG1           CNY                             

9020268  Packaging, box, QGY                 CNY                             

9017628  z-unit M48                 CNY                             

9017330  EZ1 VRS Card V2.0            CNY                             

9020236  Kit, pipettor service, 1 ch. (25), CG1                 CNY                             

9020224  Vacuum silicone grease (1 ml vial), QXT            CNY                             

9020218  Cable, UV curly cord, QGX                 CNY                             

9020212  Switch, assembly, lid, QGX                 CNY                             

9239539  Rubber Foot, .75 base, .28 hd ht, (4)                 CNY                             

9239535  Assy, Terminated Prime Switch                  CNY                             

9239533  Fitting,10-32x1/8,Face Seal (5)                   CNY                             

9020099  Snap-Ring, TLyser 2           CNY                              【人窖蛋白(Cav-1)ELISA試劑盒現(xiàn)貨】

9239507  PCBA, MC DSP, w/USB               CNY