【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

本試劑盒僅供研究使用，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問(wèn)題請(qǐng)來(lái)電，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9238884  Stepper motor, turntable, BS96            CNY                           

9238866  BR MDx, Premium Agreement      The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238857  Basic Cover Agreement Plato3, 1yr       The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238838  #Twister I, Basic Agreement, no PM    The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.  CNY                           

9241220  QIAxcel, Installation      "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements."  CNY                           

9241195  Cable, RS232, Belkin F2N209-06-T, 1.8m           CNY                           

9241186  Sensor, interlock2, with cable, QX              CNY                           

9238831  BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238822  Rect. carrier for 1 tube 100 ml             CNY                           

9237194  Reservoir RapidPlate             CNY                           

9237171  Heater Block XYP         CNY                           

9233168  Mixer Board           CNY                           

9237154  Drive Mechanism XYP         CNY                           

9237110  Upgrade App., QIAamp 96 DNA Blood On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Blood including kit    CNY                           

9237095  Training, HiLight On-site       On-site Training 1 day HiLight      CNY                            【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】

9237084  Installation, BR plus 3 integrations        CNY                           

9236414  Plate Stacker TW II              CNY                           

9232973  Level Detector Board P7-8 channels            CNY                           

9232954  LSW Board (Level sensor waste)         CNY                           

9232921  Comparator board 1.02 w/clot detection             CNY                           

9236337  Digital Board          CNY                           

9236155  Tool, calibration, syringe, RapidPlate           CNY                           

9235702  Cables, Shaker, RCS1           CNY                           

9235626  Lead Screw Nut, MP Holder LH, Shaker8000             CNY                           

9235297  Plunger tip, syringe 250µl             CNY                           

9235122  Fuse T1A, v1.00           CNY                           

9235121  Fuse T2,5A, v1.00        CNY                           

9235119  Fuse F3.15A, v1.00              CNY                           

9238667  Front panel            CNY                           

9238646  Diode BY 500-600, 600 V 5 A              CNY                           

9238621  Power supply, motorized lid locks        CNY                            【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】

QY-x2218       BABL/C1 BABL/C小鼠13-14天胚胎細(xì)胞                                    

QY-x2219       BABL/C2 BABL/C小鼠胚胎細(xì)胞                                    

QY-x2220       BABL/3T3 BABL/C小鼠胚胎成纖維細(xì)胞                                     

QY-x2221       BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞                                       

QY-x2222       Bcap-37 人乳腺癌細(xì)胞                                   

QY-x2223       BHK 敘利亞倉(cāng)鼠腎細(xì)胞                                        

QY-x2224       BHK-21 敘利亞倉(cāng)鼠腎細(xì)胞                                   

QY-x2225       BIU-87 人淺表性膀胱癌細(xì)胞                                        

QY-x2226       BRL-3A 大鼠肝細(xì)胞                                        【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】

QY-x2227       BT-325 人腦多形性膠質(zhì)母細(xì)胞瘤細(xì)胞                                       

QY-x2228       BV-2 小鼠小腦膠質(zhì)瘤細(xì)胞                                    

QY-x2229       BxPC3 人原位胰腺癌細(xì)胞                                     

QY-x2230       C127 小鼠乳腺癌細(xì)胞                                   

QY-x2231       C6/36 白紋伊蚊細(xì)胞                                      

QY-x2232       C6-121 大鼠神經(jīng)膠質(zhì)瘤細(xì)胞                                       

QY-x2233       C6-1752 大鼠神經(jīng)膠質(zhì)瘤細(xì)胞                                      

QY-x2234       C6-64 大鼠神經(jīng)膠質(zhì)瘤細(xì)胞                                         

QY-x2235       C6-70 大鼠神經(jīng)膠質(zhì)瘤細(xì)胞                                         

QY-x2236       C6-D2B 大鼠神經(jīng)膠質(zhì)瘤細(xì)胞                                      

QY-x2237       C8166-CD4 人T細(xì)胞性白血病細(xì)胞                                    

QY-x2238       CA 人肝癌細(xì)胞                                       

QY-x2239       Caco-2 人結(jié)腸腺癌細(xì)胞                                        

QY-x2240       CAM191 EBV-轉(zhuǎn)化人淋巴細(xì)胞                                      

QY-x2241       CAM200 EBV-轉(zhuǎn)化人淋巴細(xì)胞                                      

QY-x2242       CatK2 家貓腎上皮細(xì)胞                                          【大鼠乙酰膽堿轉(zhuǎn)移酶(ChAT)ELISA試劑盒現(xiàn)貨】

QY-x2243       CatL1 家貓肺成纖維樣細(xì)胞                                         

QY-x2244       CatL2 家貓肺成纖維樣細(xì)胞                                         

QY-x2245       CatS2 家貓皮膚成纖維樣細(xì)胞                                      

QY-x2246       CFPAC-1 人胰腺癌細(xì)胞                                        

QY-x2247       CHL 中國(guó)倉(cāng)鼠肺細(xì)胞                                     

QY-x2248       CHL-Don 中國(guó)倉(cāng)鼠肺成纖維樣細(xì)胞                                    

QY-x2249       CHO 中國(guó)倉(cāng)鼠卵巢細(xì)胞                                       

QY-x2250       CHO/dhfr- 中國(guó)倉(cāng)鼠卵巢細(xì)胞,