【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】ELISA試劑盒常遇見問題及解答

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯誤的結(jié)果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運行一個空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進(jìn)行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請檢查是否正確進(jìn)行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

14。我需要用一個盤子嗎？

可靠的結(jié)果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個信息的*佳來源是目前的文獻(xiàn)，很容易通過互聯(lián)網(wǎng)在多個科學(xué)數(shù)據(jù)庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

1061122  HBV PCR REAGENT VI               CNY                             

9016585  Plate Stacker Ambient Temp, RCS1          CNY                             

9016567  Z-Rack, RCS1             CNY                             

9016534  Transportation box , Twister I           CNY                             

9016532  Transportation box 3, LiquiChip                 CNY                             

9016531  Transportation box 2, LiquiChip                 CNY                             

9016149  #App. Package, M48, Gene Expression            CNY                             

9011895  Tubing, teflon, M6, 3 x 0.5 mm, 2.5 m              CNY                             

9016514  Rect. Bucket Set (4), sealable with cap            CNY                             

9016270  Maintenance Kit, Multiflow BR MDx V2          CNY                             

9016265  MJR Microseal Pad for ALP 2296  MSP-1002          Package of 10  CNY                             

9019177  Motor - Plate lock, PyroQ96MD                CNY                              【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

9011866  Shaker adapter 8000, microplate    Adapter for accommodating four 96-well plates (or other labware with the same base dimensions) on the BioRobot 8000 4-plate high-speed shaker system; not for use with the robotic handling system    CNY                            

9011839  Tubing Set, Pipet, Immucor               CNY                             

9011833  Dilutor unit, BR8000                  CNY                             

9019136  Cable - X/Y, PyroQ96ID              CNY                             

9019121  PCB 1 - Connection, PyroQ96ID                 CNY                             

9019064  PyroMark Vac Prep Tool, PyroQ24VW               CNY                             

9019018  Board, motor driver, EZ1xl                CNY                             

9019001  Door, metal frame, EZ1xl                  CNY                             

9018944  Adapter, Millipore Extraction Plate                   CNY                             

9016001  Magnet head for medium 96 plate, BS96                 CNY                             

9015970  Tube Holder, STS MDx, 9 mm (12)            CNY                             

9015636  Fuse-2, 3.15A (for PSB board), M48                   CNY                             

9015588  EZ1 DNA TISS Card  Pre-programmed card for BioRobot EZ1 DNA Tissue Protocol        CNY                             

9015586  Lid, Cover, PlateReader            CNY                             

9015540  Belt, Y                CNY                             

9019159  PCB 1 - Connection, PyroQ96MD              CNY                              【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

9014004  Light, Barrier, Drawer, BR8 RD                 CNY                             

9021165  QIAxcel ScreenGel Software (10 licenses)                 CNY                             

9021059  Door Assy, Upper Right, DCU            CNY                             

QY-x83      Acc-3涎腺腺樣囊性癌細(xì)胞                                                        

QY-x84      HO-8910PM高轉(zhuǎn)移卵巢癌細(xì)胞                                                  

QY-x85      Acc-2涎腺腺樣囊性癌細(xì)胞                                                

QY-x86      HO-8910卵巢癌細(xì)胞                                                   

QY-x87      Tca-8113舌鱗癌細(xì)胞                                                   

QY-x88      HCC 94 [HCC941122]子宮鱗癌細(xì)胞高分化                                                 

QY-x89      A-431表皮癌細(xì)胞  CRL-1555                                               

QY-x90      HeLa 229宮頸癌細(xì)胞     CCL-2.1                                         

QY-x91      CBRH-7919肝癌細(xì)胞                                                   

QY-x92      RH-35肝癌細(xì)胞                                                    

QY-x93      SHZ-88乳腺癌細(xì)胞                                                       

QY-x94      MLTC-1睪丸間質(zhì)細(xì)胞瘤細(xì)胞       CRL-2065                                                【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

QY-x95      L6565小鼠L6565白血病克隆細(xì)胞系                                                

QY-x96      F9畸胎瘤細(xì)胞         CRL-1720                                               

QY-x97      LLC Lewis肺癌細(xì)胞                                                       

QY-x98      C127乳腺腫瘤細(xì)胞        CRL-1804                                               

QY-x99      MFC胃癌細(xì)胞                                                       

QY-x100   B16黑色素瘤細(xì)胞          CRL-6322                                               

QY-x101   B16黑色素瘤細(xì)胞          CRL-6322                                               

QY-x102   P815肥大細(xì)胞瘤細(xì)胞    TIB-64                                           

QY-x103   S-180腹水瘤細(xì)胞   CCL-8                                             

QY-x104   RM-1前列腺癌細(xì)胞                                                     

QY-x105   SAC-IIC3腹水瘤細(xì)胞                                                    

QY-x106   NG108-15 [ 108CC15 ]小鼠神經(jīng)細(xì)胞瘤與大鼠神經(jīng)膠質(zhì)瘤之融合細(xì)胞      HB-12317                                              

QY-x107   SAC-IIB2腹水瘤細(xì)胞                                                    

QY-x108   Hepa 1-6肝癌細(xì)胞          CRL-1830                                               

QY-x109   P388D1淋巴瘤細(xì)胞        CCL-46                                          

QY-x110   P3X63Ag8.653骨髓瘤細(xì)胞    CRL-1580                                                【大鼠新生甲狀腺素(NN-T4)ELISA試劑盒現(xiàn)貨】

QY-x111   YAC-1淋巴瘤細(xì)胞  TIB-160                                        

QY-x112   P3X63Ag8骨髓瘤細(xì)胞   TIB-9                                             

QY-x113   EL4. IL-2淋巴瘤細(xì)胞      TIB-181                                        

QY-x114   SP2/0骨髓瘤細(xì)胞                                                 

QY-x115   EL4淋巴瘤細(xì)胞      TIB-39