1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9236364  Sheath Filter             CNY                             

9232933  Microplate Reader(Agglutination)Module                CNY                             

9232916  Card, liquid detection 1.02                CNY                             

9232910  Board, Z1 motor                CNY                             

9236351  Tubing, Valve to injection needle              CNY                             

9236326  Sheath Pressure Sensor             CNY                             

9235760  Seal, O-ring, 3mm             CNY                             

9230926  Motor Connecting Ground Cable              CNY                             

9230545  Wanne fuer Abschirmung Plato 1xxx                 CNY                             

9235700  Power Cable 24V, RCS1             CNY                             

9230291  Tubing Guide (periscope)          CNY                             

211011     QuantiTect Virus Kit (50)          CNY                              【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】

979017     MOTT 16S Primer 2 For Sequencing                  CNY                             

59824       EpiTect Fast DNA Bisulfite Kit (50)            CNY                             

206246     Type-it Microsatellite PCR Kit (2000)                 CNY                             

9012742  Syringe pump outlet tubing (4.7 m)                   CNY                             

9012736  Peristaltic pump outlet tubing (1.0 m)              CNY                             

9012725  Syringe pump outlet for Plato           CNY                             

979001     PyroMark Q96 Plate (100)        "For sample analysis of DNA template prepared with magnetic beads for ID.

Magnetic bead technology instead of vacuum prep tool is used here"   CNY                             

9012706  Base, auto vac, BR8000  This is the robovac base for the BR8000 I. There are 5 contacts.   CNY                             

133240     NeXtalStock Sodium hydroxide (200)                 CNY                             

133233     NeXtalStock Sodium cacodyl. pH 7.5 (100)                CNY                             

9002195  RGQ 2plex, PC, PrioPLUS, basic, no PM   "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002039  Rotor-Gene Q MDx 5plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (shipping, labor, parts)" CNY                             

59436       EpiTect MethyLight PCR Kit (200)             CNY                             

971540     therascreen® UGT1A1 Pyro Kit (24)                  CNY                             

133217     NeXtalStock MOPS pH 7.5 (200)                CNY                             

51504       QIAamp DNA Stool Mini Kit (50)      For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)  CNY                              【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】

9002013  Rotor-Gene Q MDx 2plex HRM System            CNY                             

970032     PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.         CNY                             

922920     LiquiChip Filter Microplates (10)               CNY                             

292023     mericon Cattle (24)           CNY                             

292013     mericon Pig Kit (24)          CNY                             

QY-17442 Anti-PP-2A（protein phosphatase 2A catalytic subunit）蛋白質(zhì)磷酸酶-2A抗體                                                      

QY-17452 Anti-PP2Ac磷酸酯酶-2Ac抗體                                                    

QY-17462 Anti-PPAR-delta (peroxisome proliferator-activated receptor;PPAR-beta)D型-過(guò)氧化酶活化增生受體抗體                                                  

QY-17472 Anti-PPARγ (peroxisome proliferator-activated receptor-gamma)過(guò)氧化酶活化增生受體γ抗體                                               

QY-17482 Anti-PKB (Akt1/PKB)蛋白激酶B（兔來(lái)源抗體）                                               

QY-17492 anti-P-AKT1/PKB1/2/3（Thr473）磷酸化蛋白激酶B抗體（絲氨酸磷酸化位點(diǎn)：473）                                                

QY-17502 anti-PKN2 (protein kinase N2)蛋白激酶N2抗體                                                  【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】

QY-17512 Anti-PR（Progestogerone receptor）孕激素受體抗體                                                      

QY-17522 Anti-PRL (Prolactin)鼠抗人泌乳素抗體                                               

QY-17532 Anti-Prolylhydroxylase脯氨酸羥化酶抗體                                                  

QY-17542 Anti-PS-1 （Presennillin-1/S182）（NT）早老素蛋白-1抗體                                                      

QY-17552 Anti-PS-1（Presennillin-1/S182）（CT）早老素蛋白-1抗體                                               

QY-17562 Anti-PS-1（Presennillin-1/S182）（NT）早老素蛋白-1抗體                                               

QY-17572 Anti-PSA (anti-Prostate Specific Antigen (PSA) , for conjugation ａnd detection)鼠抗人前列腺特異性抗原抗體                                                       

QY-17582 Anti-PSCA (Prostate Stem Cell Antigen)抗前列腺干細(xì)胞抗原抗體                                                     

QY-17592 Anti-PSD-95突觸后密度蛋白95抗體                                                

QY-17602 Anti-P-selectinP選擇素抗體                                                

QY-17612 Anti-PTEN/MMAC1一種腫瘤抑制基因                                                       

QY-17622 Anti-PTHrP (Parathyroid Hormone- related Protein)甲狀旁腺激素相關(guān)蛋白抗體                                                     

QY-17632 Anti-PTP-1B/pTyr (Protein-tyrosine phosphatase, non-receptor :p-tyr)蛋白酪氨酸磷酸酶-1B/磷酸化酪氨酸抗體                                                 

QY-17642 anti-Parvalbumin微白蛋白/細(xì)小清蛋白抗體                                                     

QY-17652 Anti-Rad51Rad51抗體                                                 

QY-17662 Anti-RAR-α (Retinoic acid -R-α)維甲酸受體-α 抗體                                                       【大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)ELISA試劑盒現(xiàn)貨】

QY-17672 Anti-RAR-β(Retinoic acid -R-β)維甲酸受體-β抗體                                                 

QY-17682 Anti-RASSF1A（Ras association domain family 1A）Ras相關(guān)區(qū)域家族1A抗體                                               

QY-17692 Anti-Rat IgA兔抗大鼠sIgA抗體                                                 

QY-17702 Anti-Resistin抵抗素抗體                                                      

QY-17712 Anti-RhoA (RhoA protein)RhoA抗體                                                     

QY-17722 Anti-ROCK1 (Rho-associated coiled-coil containing protein kinase 1)Rho相關(guān)蛋白激酶1抗體                                                

QY-17732 Anti-ROCK2 (Rho-associated coiled-coil containing protein kinase 2)Rho相關(guān)蛋白激酶2抗體                                                

QY-17742 Anti-RRAD (Ras-related associated with diabetes )RRAD抗體