1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫作

【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

30004   Strep-Tactin Superflow Plus (10 ml)     CNY            

28706   QIAquick Gel Extraction Kit (250)   250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)    CNY            

9013181 Tubing, pump outlet, DISU, BR8000       CNY            

9011481 BGR Greiferkopf Plato 1xxx      CNY            

6000-1240U  Microplate Heater I, 240V       CNY            

9011334 Arm Mechanism, RCS1     CNY            

330514  RT² SYBR Green Fluor qPCR Mastermix (6)     CNY            

9011092 Mounting plate, power supplies, BR8000      CNY            

150023  REPLI-g Mini Kit (25)   DNA Polymerase, Buffers, ａnd Reagents for 25 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)    CNY            

9241806 Pyro Q24, Installation  Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements    CNY            

9241177 Power switch, 6A, 250VAC, QX        CNY            

9241147 Transport  lock, X axis, M48        CNY            

9241032 Tube insert, 2.0 ml,samp.carr., Qsym        CNY            

9240986 PCB, main, cooling device, Qsym     CNY             【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】

9240981 Slide block, sample tracking, Qsym      CNY            

9240974 Carrier locking, sample tracking, Qsym      CNY            

9240967 Sample tracking, Qsym       CNY            

9240963 Drop catcher, pipetting system, Qsym        CNY            

9240944 Motor, xy, portal, Qsym     CNY            

9234853 Modem, analoge ELSA     CNY            

9240763 Analog Module, F2-04AD-1, AP96      CNY            

9241376 Upgrade, Autopure LS Software v3.x  The “Upgrade, Autopure LS Software v3.x” is an on-site service for the software upgrade to the latest Autopure LS software version (currently version 3.1), the verification of the instrument performance (including the setting of database parameter), ａnd a user training. The product is available for Autopure LS instruments (Version AP-98) with a current software version lower or equal to V2.4 ａnd is performed by a QIAGEN service specialist.    CNY            

9241764 QIAgility, Premium Agreement    "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

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Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"   CNY            

9241757 Certification, QIAsymphony      CNY            

9241464 USB Memory Stick 2GB, Qsym      CNY            

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Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

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Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY             【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】

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    神經(jīng)元細(xì)胞  5 ×105 1ml            

    神經(jīng)膠質(zhì)細(xì)胞    5 × 105    1ml            

    海馬神經(jīng)元細(xì)胞  5 × 105    1ml            

    腦微血管內(nèi)皮細(xì)胞    5 × 105    1ml            

    腦成纖維細(xì)胞    5 × 105    1ml             【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】

    神經(jīng)小膠質(zhì)細(xì)胞  5 × 105    1ml            

    雪旺氏細(xì)胞  5 × 105    1ml            

    食管癌組織源性原代細(xì)胞  5 × 105/1ml                   

    胃癌組織源性原代細(xì)胞    5 × 105/1ml                   

    肝癌組織源性原代細(xì)胞    5 × 105/1ml                   

    結(jié)腸癌組織源性原代細(xì)胞  5 × 105/1ml                   

    直腸癌組織源性原代細(xì)胞  5 × 105/1ml                   

    肺癌組織源性原代細(xì)胞    5 × 105/1ml                   

    膽道癌組織源性原代細(xì)胞  5 × 105/1ml                   

    腎癌組織源性原代細(xì)胞    5 × 105/1ml                   

    膀胱癌組織源性原代細(xì)胞  5 × 105/1ml                   

    胰腺癌組織源性原代細(xì)胞  5 × 105/1ml                   

    甲狀腺癌組織源性原代細(xì)胞    5 × 105/1ml                   

    前列腺癌組織源性原代細(xì)胞    5 × 105/1ml                   

    乳腺癌組織源性原代細(xì)胞  5 × 105/1ml                   

    卵巢癌組織源性原代細(xì)胞  5 × 105/1ml                    【大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒現(xiàn)貨】

    子宮癌組織源性原代細(xì)胞  5 × 105/1ml                   

    宮頸癌組織源性原代細(xì)胞  5 × 105/1ml                   

    皮膚癌組織源性原代細(xì)胞  5 × 105/1ml                   

    膠質(zhì)瘤組織源性原代細(xì)胞  5 × 105/1ml                   

    髓母細(xì)胞瘤組織源性原代細(xì)胞  5 × 105/1ml                   

    胃癌組織源性原代成纖維細(xì)胞  5 × 105/1ml                   

    肺微血管內(nèi)皮細(xì)胞    5 × 105    1ml            

    肺大動(dòng)脈內(nèi)皮細(xì)胞    5 × 105    1ml            

    肺大動(dòng)脈平滑肌細(xì)胞  5 × 105    1ml            

    肺大靜脈內(nèi)皮細(xì)胞    5 × 105    1ml