檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【大鼠白介素16(IL-16)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠白介素16(IL-16)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠白介素16(IL-16)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【大鼠白介素16(IL-16)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

800328     Miniprep, PCR purification (Easy-Read)            CNY                             

132109     EasyXtal 15-Well DG-Tool X-Seal (100)     "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."    CNY                             

132101     NeXtal DWBlock Adhesive Foils                 CNY                             

132045     NeXtal Evolution µplate (10)             CNY                             

338172     RT2 Calibration Plate A             CNY                             

338125     384EZload Covers (4)                 CNY                              【大鼠白介素16(IL-16)ELISA試劑盒廠家】

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                             

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                              【大鼠白介素16(IL-16)ELISA試劑盒廠家】

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

9238812  Rectangular carrier, undrilled          CNY                             

9019467  PLATE GLASS SHORT SET GS              CNY                             

9019465  Pads, D=10mm, white (85), Cxx                CNY                             

9019459  Transformer (115V), HS            CNY                             

9233429  Valve, magnet, dispenser, BR8000           CNY                             

9233334  Solenoid Valve, 5-fold                CNY                             

9237332  Install, BR plus 2 integr. w. scheduling     Installation ａnd training, BioRobot System including two approved integration devices with scheduling   CNY                             

L-Glu         左旋谷氨酰胺溶液        L-Glutamine Solution, 200 mM        100 ml                                 

NEAA        100X非必需氨基酸        100X Non-Essential Amino Acids      100ml                                  

HGL PCR  人胚層檢測(cè)試劑盒         Human Germ Layer Detection Kit   50 reactions                               

Ored         油紅O染色試劑盒         Oil Red O Staining Kit       100 ml                                

StemDS    人胚胎干細(xì)胞分離液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

BPF  牛血漿纖維粘連蛋白     Bovine Plasma Fibronectin       1 mg                                    

HCF  人細(xì)胞纖連蛋白     Human Cellular Fibronectin     100 μg                             

BPV  牛血漿玻連蛋白     Bovine Plasma Vitronectin    100 μg                             

rHSA         重組人血清白蛋白         "Recombinant Human Serum Albumin

"       1g                                

BPF  牛血漿纖維粘連蛋白     Bovine Plasma Fibronectin       1 mg                                    

CCCSCK     膠原I-細(xì)胞培養(yǎng)表面包被試劑盒        Collage I-Cell Culture Surface Coating Kit         10 mg                                  

PLL   多聚賴氨酸   Poly-L-Lysine 1 mg/ml       1 ml                            

PLL   多聚賴氨酸   Poly-L-Lysine 10 mg/ml    1 ml                             【大鼠白介素16(IL-16)ELISA試劑盒廠家】

STEMium 人胚胎干細(xì)胞無(wú)血清培養(yǎng)基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     無(wú)異種人胚胎干細(xì)胞生長(zhǎng)培養(yǎng)基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-ACF  無(wú)動(dòng)物成分的人胚胎干細(xì)胞生長(zhǎng)培養(yǎng)基     Animal Component-free Human Pluripotent Stem Cell Growth Medium         500 ml                                 

StemDS    人胚胎干細(xì)胞分離液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

StemCryo 人胚胎干細(xì)胞凍存液     Human Pluripotent Stem Cell Cryopreservation Medium        50ml                                    

StemCryo 人胚胎干細(xì)胞凍存液     Human Pluripotent Stem Cell Cryopreservation Medium,       5 x 10mL                                     

MEF 小鼠胚胎成纖維細(xì)胞     Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纖維細(xì)胞－－處理  Mouse Embryonic Fibroblasts-mitomicine treated 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纖維細(xì)胞－－CD1小鼠C處理        Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse 1 x 10^6 cells/vial                              

MEF-mt    小鼠胚胎成纖維細(xì)胞－－CF1小鼠C處理         Mouse Embryonic Fibroblasts from CF1 mouse      1 x 10^6 cells/vial                                

MEF-mt    小鼠胚胎成纖維細(xì)胞－－CF1小鼠C處理         Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse    1 x 10^6 cells/vial                              

HDF-f-mt 人真皮成纖維細(xì)胞-胎兒C處理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  1 x 10^6 cells/vial                              

MEF-cm   小鼠胚胎成纖維細(xì)胞培養(yǎng)基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     堿性成纖維細(xì)胞生長(zhǎng)因子刺激的小鼠胚胎成纖維細(xì)胞條件培養(yǎng)基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

ULBC plates      干細(xì)胞超低結(jié)合培養(yǎng)板         Ultra-Low Binding Culture Plate      6-well, 1 plate                                   

ULBC plates      超低結(jié)合培養(yǎng)板（6孔）      Ultra-Low Binding Culture Plate (6-well)         "6-well, 4 plates

"                                   【大鼠白介素16(IL-16)ELISA試劑盒廠家】

ULBC plate       超低結(jié)合培養(yǎng)板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 1 plate

"                                  

ULBC plates      超低結(jié)合培養(yǎng)板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 4 plates

"