貯存溫度：2-8℃

齊一生物供應(yīng)的試劑盒現(xiàn)貨庫存，隨時下單隨時發(fā)貨.保證您的課程不耽誤，方便快捷感謝您的大力支持！

用途：本品僅供科研，不得用于其它用途。

發(fā)貨方式：專業(yè)的快遞免費為您送貨

試劑盒產(chǎn)品詳細(xì)檢測范圍說明書，隨貨附送。齊一生物銷售：021－6034 8496；181214 53965；173021 04490齊一生物專業(yè)代理多種品牌進口原裝檢測試劑盒。.齊一生物科技有限公司作為酶聯(lián)免疫金牌供應(yīng)商,憑借精湛的技術(shù),全方位的售前售后免費代測技術(shù)服務(wù),共同鑄就高品質(zhì)的ELISA產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,歡迎訂購咨詢服務(wù).

.

ELISA試劑盒常遇見問題及解答

【小鼠糞甾醇SteroidsELISA試劑盒價格】1。我必須運行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯誤的結(jié)果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運行一個空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計為*佳的性能在給定的體積

【小鼠糞甾醇SteroidsELISA試劑盒價格】尊敬的客戶您好！如需技術(shù)支持輔導(dǎo)歡迎撥打齊一生物銷售：021－6034 8496；181214 53965；173021 0449011�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

【小鼠糞甾醇SteroidsELISA試劑盒價格】13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。

14。我需要用一個盤子嗎？

可靠的結(jié)果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學(xué)波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯(lián)網(wǎng)在多個科學(xué)數(shù)據(jù)庫訪問。

18。  我光密度稍高（或低）比，是與我的試劑盒說明書。為什么？

試劑盒的光密度的物理條件，如時間和溫度的影響。我們建議您縮短或延長*終孵化與基板解決方案，以補償。

【小鼠糞甾醇SteroidsELISA試劑盒價格】19。  高背景的原因是什么？

1）     洗滌不當(dāng)：檢查洗滌液儲量和確保所有建議的洗滌步驟進行。

2）     污染基材：確保沒有污染的金屬離子或氧化劑的基質(zhì)，使用前。在酶聯(lián)免疫吸附底物的開發(fā)時間內(nèi)，保持額外的底物溶液。

3）暴露于光     基材：曝光可能會導(dǎo)致在一個藍色的基板。在黑暗中保存（瓶）的解決方案，直到準(zhǔn)備好進入板。

4）     錯的孵育時間/溫度：一般按照測試協(xié)議對于培養(yǎng)時間和溫度。然而，如果所有的威爾斯是強烈的和同樣顏色的標(biāo)準(zhǔn)稀釋系列中觀察到的強度梯度，那么它可能是必要的觀察到的顏色的底物反應(yīng)，以阻止反應(yīng)更快。

9240713  Poly Muffler Kit, AP96/196          CNY                           

9240690  Bearings, Lead Screws, AP           CNY                           

9243654  Comprehen Valid., Other Quant. Sys.    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """   CNY                           

6000-5013      LumiCheck Plate User Package            CNY                           

288103    bactotype Mycoplasma Mg/Ms PCR Kit (24)              CNY                           

1048147  QIAamp Viral RNA Mini Accessory Set        CNY                           

270043    cattletype BHV1 gB Ab (5)            CNY                           

1019992  Buffer Set (RNeasy 96 BioRobot 9604 Kit           CNY                           

273401    pigtype Toxoplasma Ab (1)           CNY                           

273505    pigtype Trichinella Ab (20)           CNY                           

9019538  ZLVBelting, flat cogged, 6T, 2.5mm, CASx         CNY                           

9020591  Tray Assembly, DML 3000           CNY                           

9021007  Motor, Pipettor X, DCU        CNY                           

9013373  Fix kit, liquid handling, BR8000            CNY                           

9013315  Motor, handler T/L, BR3000         CNY                            【小鼠糞甾醇SteroidsELISA試劑盒價格】

9013262  Cable, 24 V, power to power, 1200 mm              CNY                           

9013249  BGR Wash System        CNY                           

9013207  Service kit, accessories, BR8000          CNY                           

9013194  Tubing, L0 outlet, auto vac,BR8000 v1.00          CNY                           

9013187  Holder, retractable tubing, dispenser, B         CNY                           

800328    Miniprep, PCR purification (Easy-Read)              CNY                           

132109    EasyXtal 15-Well DG-Tool X-Seal (100)      "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."   CNY                           

132101    NeXtal DWBlock Adhesive Foils          CNY                           

132045    NeXtal Evolution µplate (10)         CNY                           

338172    RT2 Calibration Plate A         CNY                           

338125    384EZload Covers (4)           CNY                           

762431    PAXgene Blood RNA MDx Kit (4)        CNY                           

203745    QIAGEN Fast Cycling PCR Kit (1000) For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution    CNY                           

203609    HotStarTaq Plus DNA Polymerase (25000)   100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2 CNY                           

201205    Taq DNA Polymerase (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."      CNY                           

QY-x1561       鼠腹水瘤EAC-E2G8                                       

QY-x1562       小鼠淋巴瘤EL-4                                     

QY-x1563       鱗癌VX-2(兔模型)                                         

QY-x1564       鮭魚胚胎細(xì)胞CHSE                                       

QY-x1565       鮭魚胚胎細(xì)胞RTG                                         

QY-x1566       大黃魚腎細(xì)胞PCK                                         

QY-x1567       鼠腹水單核細(xì)胞瘤J774A.1                                    

QY-x1568       小鼠IL-3依賴性32D細(xì)胞                                    

QY-x1569       大黃魚EPC細(xì)胞EPC                                     

QY-x1570       菜青蟲卵巢細(xì)胞Pr-HNV8                                       【小鼠糞甾醇SteroidsELISA試劑盒價格】

QY-x1571       大鼠胰島素細(xì)胞INS-1                                   

QY-x1572       小鼠淋巴結(jié)血管上皮樣細(xì)胞SVEC4-10                                       

QY-x1573       小鼠肉瘤S37                                   

QY-x1574       小鼠乳腺EMT6                                      

QY-x1575       C3H小鼠骨肉瘤LM8                                     

QY-x1576       大鼠骨肉瘤VMR106                                      

QY-x1577       猴腎CM145                                     

QY-x1578       兔成骨CIF                                      

QY-x1579       小鼠白血病L1210                                   

QY-x1580       大鼠皮層神經(jīng)元細(xì)胞RN-c                                    

QY-x1581       大鼠主動脈平滑肌細(xì)胞RSMC                                      

QY-x1582       小鼠小膠質(zhì)瘤細(xì)胞Bv-2                                         

QY-x1583       鼠雜交骨髓瘤細(xì)胞K6H6/B5                                   

QY-x1584       大鼠腦膜細(xì)胞RMC                                        

QY-x1585       轉(zhuǎn)基因細(xì)胞3T3/Fas/com                                       

QY-x1586       大鼠肝癌細(xì)胞CRL-1548                                         【小鼠糞甾醇SteroidsELISA試劑盒價格】

QY-x1587       小鼠T淋巴細(xì)胞瘤細(xì)胞Cyc-Tag(S49)