檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標抗體復合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】標本要求：

1．標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內(nèi)進行.

【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】注意事項：

1． 試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果.

3． 各步加樣均應使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質(zhì)含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據(jù)樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數(shù)；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

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9230822  Rack for reagents,Trough (for 6 troughs)                   CNY                             

9235707  Washer Cable, RCS1                   CNY                             

9235695  Ground Wire, RCS1           CNY                             

9235332  Z-Rack Packing Cover                CNY                             

9235118  Fuse F1A, v1.00                  CNY                             

9235114  Fuse FST4A, v1.00             CNY                             

9230054  Clamp, Tubing, Z-bar                 CNY                             

9234861  Lid coupling piece for system liquid          CNY                             

9239551  Cbl Assy, XY, LED Driver             CNY                             

9238663  Hook, motorized lid lock           CNY                             

9238642  Capacitor board                 CNY                             

9238626  Opto-Encoder           CNY                              【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】

9238625  Eprom ａnd controller 4K15, Zent3C                  CNY                             

9241828  Pyro Q96 MDa, Premium Agreement      "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241122  Superlube                   CNY                             

9241007  QIArent&go - Rent out fee QIAcube                 CNY                             

9240985  PCB, drive, cooling device, Qsym              CNY                             

9240982  Sensor, x, sample tracking, Qsym              CNY                             

9240951  Support, cable, portal, Qsym            CNY                             

9234820  Manual, user, QS 4.0, Int                   CNY                             

9240768  Signal Conditioner, Centrifuge, AP96                 CNY                             

9240764  DC Output Module 8NPT, 3AMP, AP96             CNY                             

6000-5003        Duraseal Sealing Film                CNY                             

9241781  RGQ, Full Agreement, no PM  "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included" CNY                             

9241776  QIAgility, Inspection Service    "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                             

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9237126  Upgrade App., PCR Setup                  CNY                             

9237093  Training, Extension day    Additional days of training may be purchased during the week of the site visit upon request.         CNY                              【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】

9236426  Board, arm interconnect, TWI                  CNY                             

QY-x1142 人腎癌細胞系ACHN                                                    

QY-x1143 人腎癌細胞GRC-1                                               

QY-x1144 人腎上皮細胞GES                                               

QY-x1145 人腎癌Wilms細胞G401                                                      

QY-x1146 Asp2人胚胎腎細胞轉(zhuǎn)化細胞FC33                                                       【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】

QY-x1147 人腎癌細胞SK-RC-42                                                   

QY-x1148 人膀胱癌細胞EJ                                                  

QY-x1149 人膀胱癌細胞5637（HTB-9）                                                    

QY-x1150 人膀胱移行細胞癌細胞T24                                               

QY-x1151 人膀胱鱗癌細胞ScaBER                                                      

QY-x1152 人膀胱癌細胞BIU－87                                               

QY-x1153 人膽管癌細胞RBE                                              

QY-x1154 人膽管癌細胞QBC939                                                

QY-x1155 人乳腺癌細胞MCF-7                                                   

QY-x1156 人乳腺癌細胞MCF-7(NEW)                                                 

QY-x1157 人乳腺癌細胞MCF-7(OLD)                                                  

QY-x1158 人乳腺癌細胞MCF-7/ER36                                                   

QY-x1159 人乳腺癌細胞(耐阿霉素)MCF-7/ADR                                                  

QY-x1160 人乳腺癌細胞MCF-7B                                                 

QY-x1161 人乳腺癌細胞SKBr-2HL                                                       

QY-x1162 人乳腺癌細胞ZR75-1                                                   【小鼠前列環(huán)素(PGI2)ELISA試劑盒價格】

QY-x1163 人乳腺癌細胞ZR75-30                                                

QY-x1164 人乳腺癌細胞MDA-MB-231                                               

QY-x1165 人乳腺癌細胞MDA-MB-468                                               

QY-x1166 人乳腺癌細胞Hs-578T                                                

QY-x1167 人乳腺癌細胞HCC1937                                              

QY-x1168 人乳腺癌細胞MDA-MB-415