1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類(lèi)戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9010830  Einschubplatte 4DeepWellRackRose-80 1177          CNY                              【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】

981601     Rotor-Disc Heat Sealing Film (60)             CNY                             

9012923  Motor, Y, SamTrack, P3xxx                CNY                             

931236     QIAsymphony DNA Mini Kit (192)             CNY                             

133256     NeXtalStock HEPES sod. salt pH 7.4 (200)                 CNY                             

133253     NeXtalStock Sodium phosphate pH6.2 (200)            CNY                             

133247     NeXtalStock Sodium succinate pH5.5 (200)              CNY                             

929609     QX Pro Labeling Buffer (25 ml)                   CNY                             

330501     RT² SYBR Green qPCR Mastermix (8)                CNY                             

330451     RT² PreAMP cDNA Synthesis Kit (12)                 CNY                             

330431     RT² Microfluidics qPCR Reagent System           CNY                             

210210     QIAGEN OneStep RT-PCR Kit (25)    For 25 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, 5x QIAGEN OneStep RT-PCR Buffer,* dNTP Mix,** 5x Q-Solution, RNase-free water CNY                             

9012758  Magnetic Stirrer, single position               CNY                             

9010345  Cable, X, flat, 1400 mm,  (Size120)                 CNY                             

9010192  X-Top cover,  P3xx0 (9194)              CNY                             

979103     PyroMark Q96 HS Nucleotide Tip (8)       Re-usable tips for dispensing nucleotides (NDTs) for use in PyroMark Q96 MD. Each package contains 8 tips.  CNY                             

301806     HiPerFect HTS Reagent (2 x 1 ml)             CNY                              【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】

9002241  QIAsymphony SP/AS, PrioPLUS         "QIAsymphony SP/AS Instrument

Installation of QIAsymphony SP/AS  instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9012713  Z-motor (Plato 7) right              CNY                             

9012695  G-motor Plato 7                 CNY                             

57704       QIAamp MinElute Virus Spin Kit (50)       For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Collection Tubes (2 ml)        CNY                             

929554     QX DNA Size Marker 50 bp-1,5 kb (100 µL)               CNY                             

764133     PAXgene Bone Marrow RNA Kit (30)       "For storage ａnd transport of bone marrow samples ａnd stabilization ａnd purification of intracellular RNA

Integrated system for storage, stabilization, ａnd purification

Immediate stabilization of intracellular RNA at collection

Rapid purification from heterogeneous bone marrow samples

Safe ａnd easy transportation"          CNY                             

762174     PAXgene Blood RNA Kit (50), CE                CNY                             

203646     HotStarTaq Plus Master Mix Kit (2500)   HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience.   CNY                             

203605     HotStarTaq Plus DNA Polymerase (1000)         4 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2         CNY                             

201443     Taq PCR Master Mix Kit (250) 3 x 1.7 ml Taq PCR Master Mix‡ containing 250 units Taq DNA Polymerase total, 3 x 1.7 ml distilled water     CNY                             

180964     GeneRead Gene Panel Mastermix (4.8 ml)               CNY                             

180485     GeneRead DNA Library L Amp Kit (100)           CNY                             

180455     GeneRead DNA Library I Amp Kit (100)            CNY                             

159205     QIAcard FTA Four Spots (100)           CNY                             

159203     QIAcard FTA Two Spots (100)            CNY                             

QY-19322 Anti-Visfatin-1/PBEF1(NT)內(nèi)脂素/內(nèi)臟脂肪素/腹脂素/內(nèi)肥素（小鼠抗人、大、小鼠、猴：N端多肽）                                                  

QY-19332 Anti-Visfatin-1/PBEF1(NT) hu、 mo、 rat、MK內(nèi)脂素/內(nèi)臟脂肪素(N端抗體)                                                      

QY-19342 Anti-Visfatin-1/PBEF1(NT) Human內(nèi)脂素/內(nèi)臟脂肪素（N端抗體）                                               

QY-19352 Anti-Visfatin-2(CT)hu、mo、rat、 MK內(nèi)脂素/內(nèi)臟脂肪素（C端抗體）                                                       

QY-19362 Anti-VSIG4 (V-set ａnd immunoglobulin domain-containing protein 4)AntibodyT淋巴細(xì)胞負(fù)調(diào)節(jié)蛋白抗體                                                     

QY-19372 Anti-VTG(vitellogenin)卵黃蛋白原抗體                                              

QY-19382 Anti-VWF (Von Willebrand Factor)血管假性血友病因子/血管性血友病因子抗體                                                   

QY-19392 Anti-WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一種新的絲氨酸/蘇氨酸激酶家族成員的基因WNK4抗體                                                      

QY-19402 Anti-WWOX （WW domain-containing oxidoreductase）包含氧化還原酶的WW域抗體                                                      

QY-19412 Mouse Anti-YERSINIA PESTIS Monoclonal Antibody鼠抗鼠疫F1蛋白/鼠疫耶爾森氏菌莢膜抗原F1單克隆抗體                                                

QY-19422 anti-XIAP（X-linked Inhibitor of Apoptosis Protein）X-連鎖凋亡蛋白/性連鎖凋亡抑制蛋白抗體                                                    【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】

QY-19432 anti-XAF1（XIAP-associated factor 1）XIAP相關(guān)因子1抗體                                                    

QY-19442 Anti-XRCC1（X-ray Repair Cross Complementing 1）X射線修復(fù)交叉互補(bǔ)蛋白/細(xì)胞堿基切除修復(fù)基因抗體                                                       

QY-19452 Anti-ZCWCC1(Zinc finger CW-type coiled-coil domain protein 1)ZCWCC1抗體                                                    

QY-19462 Anti-Z-DEVD-FMK(CASPASE3/CPP32 INHIBITOR W-3)CASPASE3凋亡抑制劑                                                       

QY-19472 Anti-ZNF268 (zinc finger protein ZNF268)- middle (1b)鋅指脂蛋白-1b抗體                                              

QY-19482 Anti-ZNF268 (zinc finger protein ZNF268)- middle (1c)鋅指脂蛋白-1c抗體                                               

QY-19492 Anti-ZNF268 (zinc finger protein ZNF268)- N-terminal (1a) Anti-ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)鋅指脂蛋白-1a抗體                                                

QY-19502 Anti-ZNF300 (zinc finger protein ZNF300)- middle鋅指蛋白300抗體                                               

QY-19512 anti-ZO-1（zonula occludens-1）胞質(zhì)緊密粘連蛋白1抗體                                                      

QY-19522 Anti-β-Actin/HRP辣根過(guò)氧化物酶標(biāo)記β-肌動(dòng)蛋白（抗體）                                              

QY-19532 Anti-人T亞群/NK細(xì)胞檢測(cè)試劑盒人T淋巴細(xì)胞亞群檢測(cè)盒 （T-SuRCet）                                                

QY-19542 CD2AP (CD2-associated protein)白細(xì)胞分化抗原CD2AP                                                    

 QY-02000         新的抑癌基因Brg1抗體      Anti-Brg1（brahma-related gene 1）                                       

 QY-02001          抗布魯氏菌抗體     Anti-Brucella                                       

 QY-02002          乳腺癌轉(zhuǎn)移抑制基因1抗體          Anti-BRMS-1 (Breast cancer metastasis-suppressor 1)                                       

 QY-02003          兔抗牛血清白蛋白抗體         Anti-BSA                                       【小鼠P物質(zhì)(SP)ELISA試劑盒價(jià)格】

 QY-02004          膽汁酸鹽輸出泵蛋白抗體     Anti-BSEP(Bile salt export pump)                                        

 QY-02005          B細(xì)胞遷移基因2抗體           Anti-BTG2/TIS21(B-cell translocation gene 2)                                              

 QY-02006          過(guò)敏毒素C5a（補(bǔ)體C5a）抗體   Anti-C5a