1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術(shù)指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標本制備要規(guī)范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術(shù)支持聯(lián)系

【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標及其它特殊要求。

【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細內(nèi)容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9001297  QIAsymphony SP                CNY                              【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】

130919     NeXtal DWBlock Nucleix Suite                   CNY                             

4811014  careHCV RT-PCR ASSAY V2                CNY                             

4857021  careMP PCR ASSAY           CNY                             

130825     NeXtal CubicPhase 3w Mo/C 8% µplate (2)               CNY                             

4555205  artus M.tuberculosis RG PCR Kit (96) RUO                CNY                             

130728     Nextal Tubes CubicPhase I Suite                CNY                             

4513263  artus HI Virus-1 RG RT-PCR Kit (24) CE             CNY                             

130719     NeXtal Tubes Nucleix Suite                CNY                             

129834     GelPilot Small Fragment Agarose (500g)                   CNY                             

129814     GelPilot LE Agarose (500g)                CNY                             

129046     MaXtract High Density (200 x 1.5 ml)               CNY                             

4504163  artus Parvo B19 TM PCR Kit (24) CE                  CNY                             

79000       Oligotex Suspension (0.5)         0.5 ml for mRNA isolation from up to 8 mg of total RNA         CNY                             

76106       RNAlater (250ml)     250 ml RNAlater RNA Stabilization Reagent for stabilization of RNA in 125 x 200 mg tissue samples         CNY                             

75142       RNeasy Midi Kit (10)         10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents ａnd Buffers   CNY                             

27191       QIAprep 96 Turbo Miniprep Kit (4)  For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps  CNY                              【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】

74004       RNeasy Micro Kit (50)      50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents ａnd Buffers  CNY                             

19504       QIAvac 96         Vacuum manifold for processing  QIAGEN 96-well plates: includes QIAvac 96 Top Plate, Base, Waste Tray, Plate Holder, Rack of Collection Microtubes (1.2 ml)       CNY                             

19083       Buffer PM (500ml)   500 ml Binding Buffer      CNY                             

9015341  Board, IRB                 CNY                             

133046     NeXtalStock Ferric chloride  (200)          CNY                             

9012227  Shaker, Tube rack for Plato 7            CNY                             

9012140  Gripper, right, handler, BR8000                CNY                             

9001670  PyroMark Q24 MDx Instrument      For methylation ａnd mutation analysis using Pyrosequencing technology in 24 well CE-marked.      CNY                             

9001660  Rotor-Gene Q 6plex System              CNY                             

9001649  Rotor-Gene Q 5plex (w/o PC) PrioPLUS   Rotor-Gene Q 5plex (w/o PC) PrioPLUS includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 3 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9001648  Rotor-Gene Q 5plex (w/o PC) Priority      Rotor-Gene Q 5plex (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

950263     Final Wash Reagent (VXF) (350 ml)          CNY                             

37561       Qproteome Sialic Glycoprotein Kit           CNY                             

133029     NeXtalStock Cesium chloride (200)           CNY                             

133021     NeXtalStock CABS untit. (200)          CNY                             

QY-18422 Anti-α-Synuclein核突觸蛋白-α抗體                                                

QY-18432 Anti-α-Synuclein核突觸蛋白/突觸素-1抗體                                                     

QY-18442 Anti-T3（TRIIODOTHYROXINE）三碘甲狀腺素T3抗體                                                    

QY-18452 Anti-T3（Triiodothyroxine）三碘甲狀腺素T3抗體                                                   

QY-18462 Anti-Tanis/SELS(Selenoprotein S)炎癥負調(diào)控因子蛋白抗體                                               【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】

QY-18472 Anti-Tau protein微管相關(guān)蛋白抗體                                                    

QY-18482 Anti-Tau protein微管相關(guān)蛋白抗體                                                    

QY-18492 Anti-P-Tau protein磷酸化微管相關(guān)蛋白抗體                                                     

QY-18502 Anti-TBX2（T-box Protein 2）新型抑癌基因抗體(一種新發(fā)現(xiàn)的抑癌基因)                                                    

QY-18512 anti-TCF7L2(transcription factor 7-like 2)轉(zhuǎn)錄因子7類似物2抗體                                                  

QY-18522 Anti-TDP-43/TARDBP (tar DNA binding protein)Tar DNA 結(jié)合蛋白43抗體                                                       

QY-18532 Anti-Tenascin固生蛋白抗體                                                

QY-18542 Anti-Tenascin/TN細胞粘合素抗體                                                       

QY-18552 Anti-TFF3 (trefoil factor3)三葉肽因子3抗體                                                       

QY-18562 Anti-TFPI-2 (tissue factor pathway in hibitor-2)組織因子途徑抑制劑-2抗體                                                     

QY-18572 Anti-TGF-α (Ttansforming Growth Factor –α)轉(zhuǎn)移生長因子–α抗體                                               

QY-18582 Anti-TGF-β1（Ttansforming Growth Factor –β1）轉(zhuǎn)移生長因子–β1抗體                                                     

QY-18592 Anti-TGF-β2 （Ttansforming Growth Factor –β2）轉(zhuǎn)移生長因子–β2抗體                                                   

QY-18602 Anti-TGF-β3（Ttansforming Growth Factor –β3）轉(zhuǎn)移生長因子–β3抗體                                                     

QY-18612 Anti-TGF-βR 2（Transforming growth factor β receptor 2 ）轉(zhuǎn)移生長因子–β受體2抗體                                                   

QY-18622 Anti-TGF-βR1/ALK (TGF-beta receptor type I)轉(zhuǎn)移生長因子–β受體1抗體                                                  【小鼠乙酰膽堿受體抗體(AChRab)ELISA試劑盒廠家】

QY-18632 Anti-Thioster-containing protein 1（Anti-Anopheles stephensi protein 1）硫酯包含蛋白-1抗體                                                    

QY-18642 anti-Thioredoxin 1(ERdj5)硫氧還蛋白抗體                                                  

QY-18652 Anti-Thy-1/CD90CD90抗體                                                   

QY-18662 Anti-Thymosin α-1胸腺肽 α-1抗體                                               

QY-18672 Anti-Thyroxine(T4)/Tg4-Ab (Thyroglobulin 4 Antibody)甲狀腺素T4抗體                                                    

QY-18682 anti-Tie2（Porcine tyrosine kinase with immunoglobulin-like ａnd EGF-like domains 2）上皮生長因子樣域酪氨酸激酶2                                                    

QY-18692 Anti-TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )兔抗人TIGAR蛋白抗體                                               

QY-18702 Anti-TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金屬蛋白酶組織抑制因子-1抗體                                                  

QY-18712 Anti-TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金屬蛋白酶組織抑制因子-2抗體