ELISA 檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

注意事項(xiàng)：

◆   每個(gè)標(biāo)本量收集體積＝  100ul × 檢測(cè)種類，如要做復(fù)孔，標(biāo)本量收集體積＝  100ul × 檢測(cè)種類×2 。

◆   對(duì)于作某一個(gè)具體指標(biāo)來(lái)說(shuō)，先用一系列稀釋度作一批標(biāo)本測(cè)定，得出對(duì)實(shí)驗(yàn)有意義的一個(gè)稀釋度（即測(cè)定劑量反應(yīng)曲線），然后用一個(gè)*適稀釋度測(cè)定即可。

◆   收集標(biāo)本前必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本，儲(chǔ)存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，將標(biāo)本及時(shí)分裝后放在-20℃ 或-70℃ 條件下保存。避免反復(fù)凍融。標(biāo)本 2 -8 ℃ 可保存48 小時(shí).-20℃ 可保存1個(gè)月。 -70 度可保存6個(gè)月。部分激素類標(biāo)本需添加抑肽酶。

◆   血清標(biāo)本采集時(shí)，應(yīng)注意避免溶血，紅細(xì)胞溶解時(shí)會(huì)釋放出具有過(guò)氧化物酶活性的物質(zhì)，以 HRP為標(biāo)記的 ELISA 測(cè)定中，溶血標(biāo)本可能會(huì)增加非特異性顯色。

【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】◆   為了保證尿液檢測(cè)結(jié)果的準(zhǔn)確性，必須正確收集尿液標(biāo)本和保存。盛尿容器要清潔干燥。使用一次性的容器（如塑料尿杯），避免因用藥并清洗不干凈而造成的污染，影響檢測(cè)結(jié)果。尿液標(biāo)本必須新鮮，留取后，應(yīng)及時(shí)檢測(cè)或保存，以免細(xì)菌繁殖。因在室溫中久置后（尤其是夏季）尿中磷酸鹽等可析出結(jié)晶而干擾檢測(cè)。

◆    標(biāo)本宜在新鮮時(shí)檢測(cè)。如有細(xì)菌污染，菌體中可能含有內(nèi)源性 HRP，也會(huì)產(chǎn)生假陽(yáng)性反應(yīng)。保存過(guò)久可發(fā)生聚合在 ELISA中可使本底加深。

◆   凍結(jié)標(biāo)本融解后，蛋白質(zhì)局部濃縮，分布不均，應(yīng)充分混勻宜輕緩，避免氣泡，可上下顛倒混和，不要在混勻器上強(qiáng)烈振蕩。

◆   混濁或有沉淀的標(biāo)本應(yīng)先離心或過(guò)濾，澄清后再檢測(cè)。

◆   反復(fù)凍融會(huì)使蛋白效價(jià)降低，所以待測(cè)標(biāo)本如需保存作多次檢測(cè)，宜少量分裝冰存。也可加入適當(dāng)防腐劑。

◆   如果您在實(shí)驗(yàn)中遇到任何特殊問(wèn)題，敬請(qǐng)咨詢 021-60348496標(biāo)本類型

ELISA常見(jiàn)的標(biāo)本一般包括：

◆   液體類標(biāo)本：包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清等。

◆   培養(yǎng)細(xì)胞

◆   組織標(biāo)本

這些標(biāo)本收集的時(shí)間、方法和保存都有一定的要求。

【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】標(biāo)本的保存

一般說(shuō)來(lái)，在 5 天內(nèi)測(cè)定的標(biāo)本可放置于 4℃，超過(guò)一周測(cè)定的需低溫凍存。

對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本，儲(chǔ)存在  4 ℃ 備用，如有特殊原因需要周期收集標(biāo)本， 將標(biāo)本及時(shí)分裝后放在  -20 ℃ 或  -70 ℃ 條件下保存。避免反復(fù)凍融。 標(biāo)本  2 -8 ℃ 可保存  48 小時(shí)，-20 ℃ 可保存  1 個(gè)月。-70 度可保存  6 個(gè)月。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。標(biāo)本的處理 可用作 ELISA 測(cè)定的標(biāo)本十分廣泛，體液（如血清）、分泌物（唾液）和排泄物（如尿液）、細(xì)胞培養(yǎng)上清、細(xì)胞、組織等均可作標(biāo)本以測(cè)定其中某種成份。有些標(biāo)本可直接進(jìn)行測(cè)定，有些則需經(jīng)預(yù)處理。

◆ 血清：  

室溫血液自然凝固  10-20 分鐘后，離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。收集上清。如有沉淀形成，應(yīng)再次離心。  

◆ 血漿：  

應(yīng)根據(jù)試劑盒的要求選擇  EDTA 、檸檬酸鈉或肝素作為抗凝劑，加入  10 ％（  v/v ）抗凝劑 ( 0.1M 檸檬酸鈉或  1% heparin 或  2.0%EDTA.Na2) 混合  10-20 分鐘后，離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清如有沉淀形 成，應(yīng)再次離心。  

【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】◆ 尿液、胸腹水、腦脊液：  

用無(wú)菌管收集。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。  

◆ 細(xì)胞培養(yǎng)上清：  

檢測(cè)分泌性的成份時(shí)，用無(wú)菌管收集。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清。檢測(cè)細(xì)胞內(nèi)的 成份時(shí)，用  PBS （  PH7.2-7.4 ）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到  100 萬(wàn) /ml 左右。通過(guò)反復(fù)凍融，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心  20 分鐘左右（  2000-3000 轉(zhuǎn) / 分）。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成，應(yīng)再次離心。  

◆ 細(xì)胞：

檢測(cè)細(xì)胞的成份時(shí)，對(duì)于貼壁細(xì)胞，去除培養(yǎng)液，用PBS、生理鹽水或無(wú)血清培養(yǎng)液洗一遍。加入適量裂解液。用槍吹打數(shù)下，使裂解液和細(xì)胞充分接觸。通常裂解液接觸細(xì)胞10 秒后，細(xì)胞就會(huì)被裂解。對(duì)于懸浮細(xì)胞，離心收集細(xì)胞，用PBS、生理鹽水或無(wú)血清培養(yǎng)液洗一遍.加入適量裂解液, 用槍吹打把細(xì)胞吹散。用手指輕彈以充分裂解細(xì)胞。充分裂解后應(yīng)沒(méi)有明顯的細(xì)胞沉淀。如果細(xì)胞量較多，必需分裝然后再裂解。充分裂解后，10000-14000g離心3-5 分鐘，取上

清，即可進(jìn)行后續(xù)操作。

【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】◆ 組織標(biāo)本：  

切割標(biāo)本后，稱取重量。加入一定量的  PBS或組織蛋白萃取試劑，緩沖液中可加入蛋白酶抑制劑。用手工或勻漿器將標(biāo)本勻漿充分。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清置于  -20 度或  - 70 度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測(cè)，其余冷凍備用。齊一生物銷售：021－6034 8496；181214 53965；173021 04490  

293023    mericon Corn (24)         CNY                            【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】

292055    mericon Goat Kit (96)           CNY                           

929521    QX Alignment Marker 15 bp/1 kb (1.5 ml)          CNY                           

9012598  Disposable Filter-Tips, 1100µl (960)     Conducting disposable filter-tips    CNY                           

206152    QIAGEN Multiplex PCR Plus Kit (100)        CNY                           

291115    mericon RR Soy Kit (96)              CNY                           

291065    mericon GMO Screen bar (96)            CNY                           

9001933  DML 3000 120-240V Q        CNY                           

9001915  PyroMark Q96 ID, Priority    PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

133120    NeXtalStock Tricine (200)           CNY                           

133180    NeXtalStock Citric acid pH 3.0 (200)          CNY                           

9018503  Manual air supply, service tool, QX             CNY                           

51161      QIAamp 96 DNA Blood Kit (4)     For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                           

133106    NeXtalStock Sodium ac. H2O pH 4.6 (200)        CNY                           

9015519  Spring, for door            CNY                           

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority     QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001875  EZ1 Advanced XL, Priority    EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                            【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】

959236    MagAttract RNA Tissue Mini M48 Kit (192)        CNY                           

956336    MagAttract RNA Univ. Tissue M48 Kit(192)        CNY                           

9018457  Cartridge, alignment tool, QX              CNY                           

9015467  Motor, P         CNY                           

9015451  Modules, DISU, BR8000 v2.1              CNY                           

9012427  Thermostate system, complete, 120V, BR30        CNY                           

9018483  Notebook, QX              CNY                           

51297     FlexiGene DNA AGF3000 Kit (640)      "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"   CNY                           

51185      QIAamp DNA Blood Midi Kit (100)      For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) CNY                           

38075     AIL Cartridge (5ml)              CNY                           

9015471  Sensor, photo, PM-U24         CNY                           

956034    EZ1 RNA Universal Tissue Kit (48)      Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.      CNY                           

204772    Rotor-Gene Multiplex PCR Kit (80)      The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.  CNY                           

9018435  Tubing, external N2 source, 10ft, QX          CNY                           

9018417  Cover, front, blue window, QX            CNY                           

QY-10622       Anti-Caspase-6 （NT）半胱胺酸蛋白酶蛋白-6抗體(N端)                                        【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】

QY-10632       Anti-Caspase-8 (pro Mch5)半胱氨酸蛋白酶8抗體                                     

QY-10642       Anti-Caspase-9白介素1-β轉(zhuǎn)化酶樣凋亡蛋白酶6抗體                                    

QY-10652       Anti-Caspase-9白介素1-β轉(zhuǎn)化酶樣凋亡蛋白酶6抗體                                    

QY-10662       Anti-β-catenin/β-catsβ-連環(huán)蛋白抗體                                      

QY-10672       Anti-NAIF1 protein（ CB12-327）核凋亡誘導(dǎo)因子蛋白1                                         

QY-10682       Anti-CBFA1/RUNX2 (Runt-related Transcription Factor 2)成骨特異性轉(zhuǎn)錄因子抗體                                       

QY-10692       Anti-CCK8(Cholecystokinin-8)膽囊收縮素抗體/縮膽囊素抗體(八肽)                                        

QY-10702       Anti-CCP (cyclic citrullinated peptide)兔抗瓜氨酸環(huán)肽抗體                                        

QY-10712       Anti-CCR-1(CC chemokine receptor 1)CC趨化因子受體1抗體                                        

QY-10722       Anti-CCR-2/5(CC chemokine receptor 2/5)CC趨化因子受體2/5抗體                                       

QY-10732       anti-CCR5/RANTES（regulated on activation normal T cell expressed ａnd secreted）CC趨化因子受體5/依賴激活T細(xì)胞分泌調(diào)節(jié)蛋白                                         

QY-10742       Anti-CCR-3(CC chemokine receptor 3)CC趨化因子受體3抗體                                        

QY-10752       Anti-CCR-4(CC chemokine receptor 4)CC趨化因子受體4抗體                                        

QY-10762       Anti-CCR-7(CC chemokine receptor 7) CC趨化因子受體7抗體                                       

QY-10772       Anti-CD10（Mouse Anti-Human CD10 Monoclonal Antibody）鼠抗人CD10單克隆抗體                                        

QY-10782       Anti-CD10/CALLA (Neutral Endopeptidase)CD10抗體                                         【小鼠前心鈉肽(Pro-ANP)ELISA試劑盒廠家】

QY-10792       Anti-CD105/Endoglin/TGF-β receptorCD105/轉(zhuǎn)化生長(zhǎng)因子β受體抗體                                        

QY-10802       Anti-CD117/c-kit/SCFR干細(xì)胞生長(zhǎng)因子受體/細(xì)胞表面分化抗原抗體                                     

QY-10812       Anti-CD133 antigen造血干細(xì)胞抗原CD133抗體                                       

QY-10822       anti-Syndecan-1/CD138多配體聚糖抗體                                      

QY-10832       Anti-CD14(cluster of differentiation antigen 14)CD14抗體                                   

QY-10842       anti-CD28CD28抗體                                       

QY-10852       Anti-CD144/VECD （Vascularendothelial cell cadherin/VE-cadherin）上皮型鈣粘附分子/血管內(nèi)皮鈣粘蛋白抗體                                     

QY-10862       Anti-CD147/EMMPRIN(Extracellular matrix metalloproteinase inducer)單核-巨噬細(xì)胞基質(zhì)金屬蛋白酶抗體                                         

QY-10872       anti-CD166/ALCAM(activated leukocyte celladhesion molecule)活化白細(xì)胞粘附分子抗體                                       

QY-10882       anti-ED-1單核/巨噬細(xì)胞表面特異性標(biāo)志抗原抗體                                    

QY-10892       Anti-CD167a/DDR1CD167a抗體                                   

QY-10902       Anti-CD169/Siglec-1/sialoadhesin（Sn）(Sialic acid-binding Ig-like lectin 1)CD169抗體                                   

QY-10912       Anti-CD17CD17抗體