齊一生物試劑盒組分經(jīng)過重重優(yōu)化，每個(gè)組分的穩(wěn)定性都完美的經(jīng)過高溫破壞測(cè)驗(yàn)，程度的避免了長(zhǎng)途運(yùn)輸和保存過程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標(biāo)曲.嚴(yán)格的質(zhì)控.穩(wěn)定高、易保存，操作簡(jiǎn)便，已廣泛應(yīng)用在全國(guó)免疫學(xué)檢驗(yàn)研究所的各領(lǐng)域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠C肽(C-Peptide)ELISA試劑盒廠家】齊一生物試劑盒憑借其完美的標(biāo)曲、嚴(yán)格的質(zhì)控、較高的穩(wěn)定性，在世界范圍內(nèi)的科研試劑領(lǐng)域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【小鼠C肽(C-Peptide)ELISA試劑盒廠家】試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說明書儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【小鼠C肽(C-Peptide)ELISA試劑盒廠家】齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

操作步驟
實(shí)驗(yàn)開始前，請(qǐng)?zhí)崆芭渲煤盟性噭�，試劑或樣品稀釋時(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
【小鼠C肽(C-Peptide)ELISA試劑盒廠家】注：
1. 用戶在初次使用試劑盒時(shí)，應(yīng)將各種試劑管離心數(shù)分鐘，以便試劑集中到管底。
2. 每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測(cè)量時(shí)先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā)，試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)，酶標(biāo)板加上蓋或覆膜。
4. 未使用完的酶標(biāo)板或者試劑，請(qǐng)于2-8℃保存。標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液、辣根過氧化物酶標(biāo)記親和素工作液請(qǐng)依據(jù)所需的量配置使用。請(qǐng)勿重復(fù)使用已稀釋過的標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液或、辣根過氧化物酶標(biāo)記親和素工作液。
5. 建議檢測(cè)樣品時(shí)均設(shè)雙孔測(cè)定，以保證檢測(cè)結(jié)果的準(zhǔn)確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY             【小鼠C肽(C-Peptide)ELISA試劑盒廠家】

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

9243553 #BR M96, PM Service "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9242864 QIArent&go -Rent out fee QSSP/AS Cabinet        CNY            

5000-1321   Feet Set/4, SKR1        CNY            

5000-1288   DML 2000 - Fuse 160 mA PKG 10       CNY            

5000-1287   DML 2000 - Fuse 315 mA PKG 10       CNY            

5000-1505   Switch ON-OFF 120V, HTR1        CNY            

6000-00181  Plate Washer - Replacement Tubing Kit       CNY            

6000-00178  Plate Washer - Maintenance Kit      CNY            

5100-00047  Wash Buffer Concentrate (2 Bottles) "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"   CNY            

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY             【小鼠C肽(C-Peptide)ELISA試劑盒廠家】

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY            

9019474 Lid, UV, polycarb, C1x      CNY            

9238884 Stepper motor, turntable, BS96      CNY            

9238866 BR MDx, Premium Agreement   The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY            

9238857 Basic Cover Agreement Plato3, 1yr   The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238838 #Twister I, Basic Agreement, no PM  The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9241220 QIAxcel, Installation   "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements."    CNY            

9241195 Cable, RS232, Belkin F2N209-06-T, 1.8m      CNY            

9241186 Sensor, interlock2, with cable, QX      CNY            

9238831 BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238822 Rect. carrier for 1 tube 100 ml     CNY            

MADM-b 間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基-基礎(chǔ)    Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal    500 ml             

MADM-b-prf 間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基-無酚紅  Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free    500ml              

MCDM    間充質(zhì)干細(xì)胞-軟骨分化培養(yǎng)基 Mesenchymal Stem Cell Chondrogenic Differentiation Medium   500ml              

Ared    茜素紅S染色試劑盒  Alizarin Red S Staining Kit 100 ml             

Ored    油紅O染色試劑盒    Oil Red O Staining Kit  100 ml             

PCR 人間質(zhì)干細(xì)胞成脂肪細(xì)胞檢測(cè)試劑盒    Human Mesenchymal Stem Cell Adipogenesis Detection Kit  50 reactions              

HMSC-C PCR  人間充質(zhì)干細(xì)胞軟骨形成檢測(cè)試劑盒    Human Mesenchymal Stem Cell Chondrogenesis Detection Kit    50 reactions               

SafraninO   番紅O染色試劑盒    Safranin O Staining Kit 100 reactions              

ABlue   阿利新藍(lán)染色試劑盒  Alcian Blue Staining Kit    100 reactions               【小鼠C肽(C-Peptide)ELISA試劑盒廠家】

HDF-f   人真皮成纖維細(xì)胞－－胎兒  Human Dermal Fibroblasts-fetal  5 x 10^5 cells/vial            

HDF-n   人真皮成纖維細(xì)胞－－新生  Human Dermal Fibroblasts-neonate    5 x 10^5 cells/vial            

HDF-a   人真皮成纖維細(xì)胞－－成人  Human Dermal Fibroblasts-adult  5 x 10^5 cells/vial            

HEK-n   人表皮角質(zhì)細(xì)胞－－新生    Human Epidermal Keratinocytes-neonatal  5 x 10^5 cells/vial            

AEpiCM  肺泡上皮細(xì)胞培養(yǎng)基  Alveolar Epithelial Cell Medium 500 ml             

KM  角質(zhì)細(xì)胞培養(yǎng)基 Keratinocyte Medium 500 ml             

HGL PCR 人胚層檢測(cè)試劑盒    Human Germ Layer Detection Kit  50 reactions               

HSC PCR 人干細(xì)胞多能性檢測(cè)試劑盒    Human Stem Cell Pluripotency Detection Kit  50 reactions               

ALP 堿性磷酸酶活性測(cè)定試劑盒    Alkaline Phosphatase Activity Assay 500 tests              

ALPr    堿性磷酸酶染色檢測(cè)（紅色）  Alkaline Phosphatase Staining Assay (Red)   500 tests              

EndoFectagen®   內(nèi)皮細(xì)胞轉(zhuǎn)染試劑盒 Endothelial Cell Transfection Kit   250 Transfections              

FibroFectagen® 成纖維細(xì)胞轉(zhuǎn)染試劑 Fibroblast Transfection Kit 200 Transfections              

EpiFectagen®    上皮細(xì)胞轉(zhuǎn)染試劑盒  Epithelial Cell Transfection Kit    100 Transfections              

MesenFectagen® 間充質(zhì)干細(xì)胞轉(zhuǎn)染試劑盒  Mesenchymal Stem Cell Transfection Kit  250 Transfections              

AstroFectagen® 星形膠質(zhì)細(xì)胞轉(zhuǎn)染試劑盒      Astrocyte Transfection Kit  250 Transfections              

SMCFectagen®    平滑肌細(xì)胞轉(zhuǎn)染試劑盒    Smooth Muscle Cell Transfection Kit 250 Transfections               【小鼠C肽(C-Peptide)ELISA試劑盒廠家】

KeratoFectagen®     角質(zhì)細(xì)胞轉(zhuǎn)染試劑盒 Keratinocyte Transfection Kit   200 Transfections              

EpiFectagen II®      上皮細(xì)胞轉(zhuǎn)染試劑盒     Epithelial Cell Transfection Kit    250 Transfections   無貨           

IVTFA   體外管腔形成分析試劑盒 In Vitro Tube Fomation Assay (Angiogenesis Assay)   50 tests/96 well plate               

Col I型膠原細(xì)胞粘附檢測(cè)試劑盒      Collagen I Cell Adhesion Assay  48 tests/48 well plate             

Fibro   纖維粘連細(xì)胞粘附檢測(cè)試劑盒 Fibronectin Cell Adhesion Assay 48 tests/48 well plate