檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

5126-1220        hc2 DNA Collection Device                CNY                              【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】

GR8RNA  QIAshredder (250)/RNeasy (250) spezial                   CNY                             

RNEZ4U   QIAshredder (50)/RNeasy (50) special              CNY                             

9240736  Decon tube, Actril, AP98          CNY                             

5000-1410        Strip Shock Absorbing, VTX2             CNY                             

9240692  Fan, 12Vdc, PC-104 cover, AP196/98                  CNY                             

51331       QIAamp 96 DNA QIAcube HT Kit (5)                  CNY                             

74171       RNeasy 96 QIAcube HT Kit (5)          CNY                             

180994     GeneRead Adapter L Set 12-plex (72)               CNY                             

9002140  careHPV test Luminometer              CNY                             

9230355  Washstation P1xxx            CNY                             

9235295  Sound absorber (muffler)                   CNY                             

9235277  Board, HS shaker, BR8000                 CNY                             

9230202  Safety shield for size1                CNY                             

9234858  Toubing coupling piece (washer tubing)           CNY                             

9238675  Operating mechanism,  left mech. lock                   CNY                             

9238655  Computer board cable, 3-channel            CNY                              【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】

9238633  Grey wedge (long)   " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor.

SIGMA sensor"         CNY                             

9238622  Main board Zent3, no Eprom (>SN85353)                 CNY                             

9241891  QIArent&go - Rent out fee Rotor-Gene           CNY                             

9241823  Pyro Q96 ID, Full Agreement   The Full Cover Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.         CNY                             

9241816  Pyro Q96 MD, PM Agreement                   CNY                             

9241807  Pyro Q96 ID, Installation Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements         CNY                             

9241125  Thermal Joint Compound                   CNY                             

9240978  PCB, connector, sample tracking, Qsym           CNY                             

9240947  Encoder, x, portal, Qsym          CNY                             

9234821  Manual, user, QS 4.0, US          CNY                             

9240785  Reagent #1 Manifold Update, AP98                  CNY                             

9241784  QIAxcel, Training      "The Training, QIAxcel is a 2 days on-site service for up to four QIAxcel users, conducted by a QIAGEN Field Service Specialist. It includes an introduction to operation of the QIAxcel system hardware ａnd the usage of the BioCalculator software. It covers the preparation of sample material, run preparation, data acquisition, ａnd data evaluation. The training is performed using standardized sample material; customer samples may be processed upon request.

All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. Training certificates for participants can be issued upon request."       CNY                             

9243544  Autopure, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242713  Add. Unit, Validation Support, 2d    Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Guided or Accelerated validation service catalog no(s). Scope of work defined from the primary unit utilized in "Guided Validation Support" or "Accelerated Validation Support".  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.        CNY                             

9242545  Quick Disconnect O-ring set, LC                 CNY                             

QS-5170   串珠鐮刀菌素MON        Moniliformin                                        

QS-5171   HT-2毒素         HT-2 Toxin                                             

QS-5172   膠黏毒素         Gliotoxin                                       【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】

QS-5173   青霉酸     Penicillic acid                                        

QS-5174   青霉震顫素     Penitrem A                                           

QS-5175   疣孢青霉原     Verruculogen                                       

QS-5176   雪腐鐮刀菌烯醇     Nivalenol                                      

QS-5177   環(huán)匹阿尼酸/圓弧偶氮酸CPA         Cyclopiazonic acid                                        

QS-5178            Paxilline                                        

QS-5179   騰毒素     Tentoxin                                       

QS-5180   渥曼青霉素     Wortmannin(WT)                                         

QS-5181   霉酚酸     Mycophenolic acid(MPA) 1100(100mg)                              

QS-5182   玉米赤霉酮     Zearala    50支以下                                   

QS-5183   黃曲霉毒素免疫親和柱                   130元/支                                    

QS-5184   玉米赤霉烯酮免疫親和柱              150元/支                                    

HBMEC    人腦微血管內(nèi)皮細(xì)胞     Human Brain Microvascular Endothelial Cells 5 x 10^5 cells/vial                                

HBVSMC  人腦血管平滑肌細(xì)胞     Human Brain Vascular Smooth Muscle Cells    5 x 10^5 cells/vial                                

HBVAF      人腦血管外膜成纖維細(xì)胞     Human Brain Vascular Adventitial Fibroblasts         5 x 10^5 cells/vial                                

HBVP        人腦血管周細(xì)胞     Human Brain Vascular Pericytes      5 x 10^5 cells/vial                                 【小鼠低密度脂蛋白免疫復(fù)合物(LDL-IC)ELISA試劑盒價(jià)格】

HCPEC      人脈絡(luò)叢內(nèi)皮細(xì)胞         Human Choroid Plexus Endothelial Cells  5 x 10^5 cells/vial                                

HCPEpiC 人脈絡(luò)叢上皮細(xì)胞         Human Choroid Plexus Epithelial Cells     5 x 10^5 cells/vial                                

HCPF         人脈絡(luò)叢成纖維細(xì)胞     Human Choroid Plexus Fibroblasts  5 x 10^5 cells/vial                                

HMC         人腦膜細(xì)胞     Human Meningeal Cells   5 x 10^5 cells/vial                                

HN   人神經(jīng)元細(xì)胞        Human Neurons       1 x 10^6 cells/vial                                

HCGC        人小腦顆粒細(xì)胞     Human Cerebellar Granule Cells      1 x 10^6 cells/vial                                

HN-h         人海馬趾神經(jīng)元     Human Neurons-hippocampal          1 x 10^6 cells/vial                                

HCN         人大腦皮層神經(jīng)元         Human Cortical Neurons 1 x 10^6 cells/vial                              

HN-bs       人腦干神經(jīng)元         Human Neurons-brain stem    1 x 10^6 cells                             

HOPC        人少突膠質(zhì)前體細(xì)胞－－貼壁生長  Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial                                

HOPC-os   人少突膠質(zhì)前體細(xì)胞－－懸浮生長  Human Oligodendrocyte Precursor Cells-oligospheres    1 x 10^6 cells/vial