檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

9238960  Serial Cable, F9/F25, BS            CNY                              【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

9016903  Upgrade kit, needle, w-drive, BR8           CNY                             

9016857  XYP IS V2.3                CNY                             

9232557  Vacuum manifold, plate holder 1.02                 CNY                             

9238941  PCB, CSU-01, BS15            CNY                             

9241121  Loctite 425                CNY                             

9241120  Loctite 242                CNY                             

9240957  PCB, gripper, handler, Qsym             CNY                             

9240953  Motor, z,  handler, Qsym                 CNY                             

9240781  500 ml Reagent #6 Update, AP96            CNY                             

9240780  500 ml Reagent #6 Update, AP196          CNY                             

9240772  Assy, Sub, Dispense Head, AP96                CNY                             

9240769  Mono Touch Screen, AP96                 CNY                             

9241465  IQ/OQ Services, QIAcube         Instrument qualification support service for the QIAcube. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included). CNY                             

9243647  Acceler. Valid Supp, QuantiPlex/QP Hyres        The QIAGEN Quantiplex/Quantiplex HYres Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introductory seminar about the Quantiplex/Quantiplex HYres quantification chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through Quantiplex/Quantiplex HYres validation, as well as general wet lab support such as pipetting for quantification to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                             

9243646  Guided Valid Support, Other Quant. Sys  The QIAGEN Guided Validation Support, Other Quantification System service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)      CNY                             

9243541  #Rapid Plate, PM Service         "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                              【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

9243115  Lee Sample Valve, LC                 CNY                             

9242863  QIArent&go - Rent out fee QSAS Cabinet                  CNY                             

9242771  Tool, align encoder, Qsym                  CNY                             

9242717  Validation Support, 5d, QIAcube      The QIAGEN QIAcube Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAcube instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.). CNY                             

9242204  Adapter, 2x Rotor-Disc, Qsym          CNY                             

5000-1020R     DML 2000 Luminometer, 240V (Refurbished)          CNY                             

5000-1323        Rotary Shaker - Cord Line, 220V                CNY                             

5000-1317        Knob Speed Timer, SKR1           CNY                             

5000-1272        X Drive Track, DML2                  CNY                             

5000-1260        Stepper Model New Styl, DML2                CNY                             

5001-2240        Hybrid Capture 2 (hc2)System Core, 240V                CNY                             

5000-1506        Switch ON-OFF 240V, HTR1               CNY                             

6000-4111ENV2.2   RCS User Application Manual (English)             CNY                             

6000-3117        Plate Washer I - Maintenance Kit             CNY                             

5199-1220        hc2 High-Risk HPV DNA Test             CNY                             

QS-5139   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         50ug                                     【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

QS-5140   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         100ug                                  

QS-5141   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         250ug                                  

QS-5142   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         500ug                                  

QS-5143   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         1mg                            

QS-5144   黑海綿酸,軟海綿酸        Okadaic acid(OA)   ≥ 95% by HPLC     25ug                                    

QS-5145   黑海綿酸,軟海綿酸        Okadaic acid(OA)   ≥ 95% by HPLC     50ug                                    

QS-5146   黑海綿酸,軟海綿酸        Okadaic acid(OA)   ≥ 95% by HPLC     100ug                                  

QS-5147   黑海綿酸,軟海綿酸        Okadaic acid(OA)   ≥ 95% by HPLC     500ug                                  

QS-5148   黑海綿酸,軟海綿酸        Okadaic acid(OA)   ≥ 95% by HPLC     1mg                            

QS-5149   鰭藻毒素         Dinophysistoxins(DTX-1)    ≥ 95% by HPLC         1mg                            

QS-5150   裸藻毒     Brevetoxin B (PbTx-2)   ≥ 98% by HPLC      1mg                            

QS-5151   裸藻毒     Brevetoxin B (PbTx-2)   ≥ 98% by HPLC      5mg                            

QS-5152   石房蛤毒素     Saxitoxin acetate     1mg                            

QS-5154   黃曲霉毒素B1        Aflatoxin B1                                          

QS-5155   黃曲霉毒素B2        Aflatoxin B2                                          

QS-5156   黃曲霉毒素G1        Aflatoxin G1                                          【小鼠抗子宮內(nèi)膜抗體(EMAb)ELISA試劑盒廠家】

QS-5157   黃曲霉毒素G2        Aflatoxin G2                                         

QS-5158   黃曲霉毒素M1       Aflatoxin M1    2320                                    

QS-5159   黃曲霉毒素M2       Aflatoxin M2    6200                                    

QS-5160   桔青霉素/桔霉素   Citrinin                                          

QS-5161   嘔吐毒素/        Deoxynivalenol                                             

QS-5162   脫氧雪腐鐮刀菌烯醇DON    Vomintoxin                                           

QS-5163   伏馬毒素B1   Fumonisin B1                                        

QS-5164   伏馬毒素B2   Fumonisin B2                                        

QS-5165   赭曲霉毒素A Ochratoxin A                                        

QS-5166   棒曲霉素/展青霉素        Patulin                                          

QS-5167   T-2 毒素 T2 Toxin                                        

QS-5168   玉米赤霉烯酮/F2毒素ZEN   Zearale                                         

QS-5169   雜色曲霉素/柄曲霉素ST       Sterigmatocystin