檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

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9239855  Compressor, v2.0               CNY                             

9019494  Measurement Gauge Kit, RCS                   CNY                             

9238863  LiquiChip, Premium Agreement       The Premium Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).     CNY                             

5000-1251        Motor Control PCB, DML2                 CNY                             

5000-1513        H-Spring, DML2                 CNY                             

6000-3119        Plate Washer I - Rinse Cap Assembly                 CNY                             

5000-1417        Overlay 099A, VTX2                   CNY                             

5050-1078        LIS Information Package           CNY                             

9240717  Lead Screw Assy, Axis 5, TR, AP                 CNY                             

9240703  O-Ring, Valve, Reagent Pump, AP            CNY                             

9020806  Rotor Timing Calibration Dye, RGQ                   CNY                             

338134     Microbial DNA Positive Control                 CNY                             

288105     bactotype Mycoplasma Mg/Ms PCR Kit (96)            CNY                              【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】

275002     flocktype NDV Ab (2)                 CNY                             

285515     cador C. burnetii PCR Reagent (96)          CNY                             

285115     cador TKP PCR Reagent (96)             CNY                             

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9020592  Optical Head Assembly, DML 3000           CNY                             

9021015  Encoder, Z Axis, VR, DCU                   CNY                             

9013209  Cabling kit, 5V/24V, BR8000              CNY                             

9013189  Wheel, retractable tubing, dispenser, BR                 CNY                             

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QTR-1000         狂犬病抗原檢測(cè)試劑盒                                                       

QTR-1001         FELINE specialties貓病                                                 

QTR-1002         貓冠狀病毒抗體檢測(cè)試劑盒                                                        【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】

QTR-1003         貓白血病抗體檢測(cè)試劑盒                                                  

QTR-1004         貓白血病病毒抗原檢測(cè)試紙條                                                  

QTR-1005         貓白血病抗原p27定量檢測(cè)試劑盒                                                   

QTR-1006         "貓免疫缺陷病(Feline

Immunodefciency (FIV))

"                                                     

QTR-1007         貓免疫缺陷病抗體檢測(cè)試劑盒                                                  

QTR-1008         貓免疫缺陷病抗體檢測(cè)試紙條                                                  

QTR-1009         貓免疫缺陷病抗體定量檢測(cè)試劑盒                                                  

QTR-1010         布魯氏菌PCR測(cè)試劑盒         24次                                             

QTR-1011         副結(jié)核分枝桿菌PCR檢測(cè)試劑盒         100次                                          

QTR-1012         支原體PCR檢測(cè)試劑盒         50次                                             

QTR-1013         瘟病毒PCR法檢測(cè)試劑盒     100次                                          

QTR-1014         偽狂犬PCR檢測(cè)試劑盒         100次                                          

QTR-1015         非洲豬瘟PCR檢測(cè)試劑盒     100次                                          

QTR-1016         豬布病PCR檢測(cè)試劑盒         50次                                             

QTR-1017         螺旋體PCR檢測(cè)試劑盒         50次                                             

QTR-1018         胞內(nèi)勞森菌PCR檢測(cè)試劑盒         50次                                              【小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒價(jià)格】

QTR-1019         胞內(nèi)勞森菌PCR檢測(cè)試劑盒         100次                                          

QTR-1020         豬圓環(huán)PCR檢測(cè)試劑盒         100次                                          

QTR-1021         豬細(xì)小病毒PCR試劑盒         100次                                          

QTR-1022         豬胃腸炎/呼吸道冠狀病毒PCR試劑盒        50次                                             

QTR-1023         豬藍(lán)耳病毒PCR試劑盒         50次                                             

QTR-1024         豬藍(lán)耳-圓環(huán)二聯(lián)PCR試劑盒        50次                                             

QTR-1025         牛傳染性鼻氣管炎PCR試劑盒100次                                                       

QTR-1026         彎曲桿菌PCR試劑盒     100次                                          

QTR-1027         傳染性法氏囊PCR試劑盒     100次                                          

QTR-1028         傳染性支氣管PCR試劑盒     100次                                          

QTR-1029         傳染性喉氣管炎PCR試劑盒         50次