價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計(jì)算時(shí)請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時(shí)，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9012331  Set, Tubing, Pipetting, Plato3xx1              CNY                              【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

133084     NeXtalStock PEG 300 (200)               CNY                             

38065       #PNA Cartridge (5ml)                CNY                             

204752     QuantiFast Multiplex PCR +R Kit (80)                CNY                             

290095     mericon Quant. L. pneumophila Kit (96)          CNY                             

800423     Library, Large Insert, Picked Clones                   CNY                             

290045     mericon Campylobacter triple Kit (96)              CNY                             

9015353  Controller, temp.               CNY                             

133051     NeXtalStock HEPES sod. salt untit. (200)          CNY                             

9018388  QIAcube Tip Adapter Ring Tool                 CNY                             

9018377  Teaching Tools, Alignment, AP                   CNY                             

9012226  Power Supply, 6 amp for Plato size 90               CNY                             

9012066  Tray, buffer bottles, roter, BR8000           CNY                             

9001674  PyroMark Q96 MD Automated Instrument     For performing automated pyrosequncing reaction on the Q96 MD      CNY                             

9001658  Rotor-Gene Q 5plex HRM (w/o PC) Priority     Rotor-Gene Q 5plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

951054     EZ1 DNA Blood 350 µl Kit (48)          "

48 Reagent Cartridges (Blood 350 ul), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml)"  CNY                             

9001874  EZ1 Advanced XL, System         EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

9015466  Cover, P             CNY                             

9012413  Z-Bars Gripper, RCS1                 CNY                             

9012410  Tischplatte Plato 3301S Technikk  (9194)                CNY                             

205110     Omniscript RT Kit (10)      For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

204845     QuantiTect Multiplex RT-PCR NR Kit(1000)               CNY                             

133178     NeXtalStock Citric acid pH 2.6 (200)                  CNY                             

133165     NeXtalStock CAPS pH 11.4 (200)               CNY                             

9018409  Regulator, N2, internal, front, QX            CNY                             

9015406  Driver, motor, M96           CNY                             

9001861  Rotor-Gene Q 2plex (PC) Priority     Rotor-Gene Q 2plex (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9012327  Peristaltic Pump Outlet Tubing                  CNY                             

1067925  Buffer AW1 (175 ml)                  CNY                             

38045       GNA Cartridge (5ml)                  CNY                             

38003       ConA Cartridges (3 x 1ml)                  CNY                             

9015365  Drop catcher, M96           CNY                             

QYD896   餐具大腸菌群快速檢驗(yàn)紙片         100次/袋                                             

QYHP001 小鼠IgA類單克隆抗體腹水純化試劑盒                                        盒                                                  

QYHP002 小鼠IgM類單克隆抗體腹水純化試劑盒                                       盒                                                  

QYHP003 IgG(1、2a、2b) 類單克隆抗體腹水純化試劑盒                                   盒                                                  

QYHP004 小鼠IgG3類單克隆抗體腹水純化試劑盒                                      盒                                                  

QYHP005 小鼠單克隆抗體Ig類/亞類鑒定ELISA試劑盒                                96孔板X2                                         

QYHP006 半抗原偶聯(lián)試劑盒（EDC法，500MG載體蛋白量）                          盒                                            

QYHP007 HRP抗體標(biāo)記試劑盒   （優(yōu)化高碘酸鈉氧化法）                                                盒                                          

QYH01      醛固酮檢測試劑盒（血清或尿液）     Aldosterone     96孔/盒                             

QYH02      5α－雄甾－3α，17β－二醇葡萄糖醛苷酸檢測試劑盒（血清）         5a-Androstane-3a, 17b diol Glucuronide (3a-Diol G)     96孔/盒                             

QYH03      雄烯二酮檢測試劑盒（血清）     Androstenedione     96孔/盒                             

QYH04      雄烯二酮（唾液）檢測試劑盒（血清）     Androstenedione Saliva   96孔/盒                             

QYH05      抗甲狀腺過氧化物酶自身抗體（Anti-TPO）檢測試劑盒（血清）  Anti-Thyroid Peroxidase Autoantibodies (Anti-TPO)         96孔/盒                             

QYH06      抗甲狀腺球蛋白自身抗體（Anti-TG）檢測試劑盒（血清）    Anti-Thyroglobulin Autoantibodies (Anti-TG)  96孔/盒                             

QYH07      β2微球蛋白（β2M）檢測試劑盒（血清）    B2Microglobulin       96孔/盒                              【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

QYH08      C肽檢測試劑盒（血清）      C-Peptide 96孔/盒                             

QYH09      C反應(yīng)蛋白（hs-CRP）檢測試劑盒（血清）       C-Reactive Protein (hs-CRP)     96孔/盒                             

QYH10      癌胚抗原（CEA）檢測試劑盒（血清）       Carcinoembryonic Antigen (CEA)     96孔/盒                             

QYH11      皮質(zhì)醇檢測試劑盒（血清）         Cortisol     96孔/盒                             

QYH12      皮質(zhì)醇（唾液）檢測試劑盒         Cortisol Saliva  96孔/盒                             

QYH13      絨毛膜促性腺激素（hCG）檢測試劑盒（血清）      Chorionic Gonadotropin (hCG)          96孔/盒                             

QYH14      脫氫表雄酮硫酸鹽（硫酸脫氫表雄酮）（DHEAS）檢測試劑盒（血清）        Dehydroepiandrosterone Sulfate (DHEAS)         96孔/盒                             

QYH15      二氫睪酮（雙氫睪酮）（DHT）檢測試劑盒（血清）        Dihydrotestosterone (DHT)     96孔/盒                             

QYH16      雌二醇檢測試劑盒（血清）         Estradiol  96孔/盒                             

QYH17      雌酮檢測試劑盒（血清）     Estrone    96孔/盒                             

QYH18      鐵蛋白檢測試劑盒（血清）         Ferritin     96孔/盒                             

QYH19      卵泡刺激素（hFSH）檢測試劑盒（血清）         Follicle Stimulating Hormone (hFSH)        96孔/盒                             

QYH20      生長激素（hGH）檢測試劑盒（血清）      Growth Hormone (hGH)  96孔/盒                             

QYH21      胰島素樣生長因子結(jié)合蛋白1（IGFBP-1）檢測試劑盒（血清）    Insulin-Like Growth Factor Binding Protein-1 (IGFBP-1)         96孔/盒                             

QYH22      促黃體生成素（hLH）檢測試劑盒（血清）       Luteinizing Hormone (hLH)      96孔/盒                             

QYH23      瘦素檢測試劑盒（血清）     Leptin       96孔/盒                              【豬高鐵血紅蛋白(MHB)ELISA 試劑盒現(xiàn)貨】

QYH24      卵巢癌（CA125）檢測試劑盒（血清）       Ovarian Cancer (CA125)  96孔/盒                             

QYH25      孕烯醇酮檢測試劑盒（血清）     Pregnenolone  96孔/盒