【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【豬白介素1β (IL-1β)ELISA 試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【豬白介素1β (IL-1β)ELISA 試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【豬白介素1β (IL-1β)ELISA 試劑盒廠家】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【豬白介素1β (IL-1β)ELISA 試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

800141    Investigation grade sequencing, w/ prep       "Single-stranded sequencing of an unknown sequence

QIAGEN DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99%"    CNY                            【豬白介素1β (IL-1β)ELISA 試劑盒廠家】

767134    PAXgene Tissue DNA Kit (50)             CNY                           

929605    QX Mineral Oil (50 ml)         CNY                           

330513    RT² SYBR Green Fluor qPCR Mastermix (24)            CNY                           

330502    RT² SYBR Green qPCR Mastermix (12)              CNY                           

330401    RT² First Strand Kit (12)              CNY                           

9010813  BGR Stirrer PK80          CNY                           

69506     DNeasy Blood & Tissue Kit (250) 250 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)       CNY                           

63204     DyeEx 2.0 Spin Kit (50) 50 DyeEx Spin Columns, Collection Tubes (2 ml)       CNY                           

211392    Internal Control DNA (High conc.)              CNY                           

9012755  Set, Tubing, Washer, P3        CNY                           

9010126  Washer boat           CNY                           

979015    MOTT 16S Primer 1 for Sequencing           CNY                           

979010    PyroMark Vacuum Prep Filter Probe (100)   Re-usable filter probes for PyroMark Vacuum Workstation Q96 ａnd Q24.       CNY                           

59720     EpiTect Fast 96 DNA Bisulfite Kit         CNY                           

9002245  QIAcube(220-240v) Promo, PrioPLUS QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.   CNY                           

9002198  RGQ 5plex HRM, PC, PrioPLUS, no PM     "RGQ 5plex, HRM, PC Instrument

Installation of RGQ 5plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                            【豬白介素1β (IL-1β)ELISA 試劑盒廠家】

9002197  RGQ 5plex, PC, PrioPLUS, basic, no PM    "RGQ 5plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No Preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                           

9012718  Pipetting Position (raised platform)              CNY                           

9002042  Rotor-Gene Q MDx 6plex Platform             CNY                           

9002038  Rotor-Gene Q MDx 5plex HRM, Priority     Rotor-Gene Q MDx 5plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

59110      EpiTect 96-well Bisulfite Kit (2)    2x EpiTect Bisulfite 96-well Plates, Reaction Mix, DNA Protect Buffer, Carrier RNA, Buffers    CNY                           

133225    NeXtalStock Sodium ac. pH 4.8 (200)         CNY                           

301305    SuperFect Transfection Reagent (1.2 ml)     For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates       CNY                           

970802    PyroMark Gold Q24 Reagents (5 x 24) For performing pyrosequncing reaction on the Q24     CNY                           

970470    BRAF Pyro Kit (24)              CNY                           

970061    MGMT Pyro Kit (48)            CNY                           

133209    NeXtalStock MES pH 5.0 (100)           CNY                           

292065    mericon Sheep Kit (96)         CNY                           

292045    mericon Turkey (96)            CNY                           

929501    QX Pro Calibration Marker (0.6 ml)            CNY                           

9012658  Cable, X-BCR board to buffer track reade           CNY                           

QYD115  豬囊蟲抗體檢測試劑盒       96孔/盒 評估豬場豬瘟疫苗免疫狀況，感染豬的血清學(xué)診斷                           

QYD116  豬輪狀病毒IgM抗體（RV-IgM)檢測試劑盒     97孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中輪狀病毒IgM 抗體（RV-IgM)表達(dá)                      

QYD117  豬大腸桿菌K99 檢測試劑盒      96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中大腸桿菌K99表達(dá)                            【豬白介素1β (IL-1β)ELISA 試劑盒廠家】

QYD118  豬大腸桿菌987P 檢測試劑盒    96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中大腸桿菌987P表達(dá)                         

QYD119  豬大腸桿菌K88 檢測試劑盒     96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中大腸桿菌K88表達(dá)                           

QYD120  豬傳染性腸胃炎病毒檢測試劑盒       96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中傳染性胃腸炎病毒(TGEV)表達(dá)                           

QYD121  豬流行性腹瀉病毒（PEDV）檢測試劑盒        96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中流行性腹瀉病毒（PEDV）表達(dá)                         

QYD122  豬輪狀病毒抗原（RV-Ag)檢測試劑盒       96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中輪狀病毒抗原（RV-Ag)表達(dá)                           

QYD123  豬圓環(huán)病毒抗原（PCV）檢測試劑盒 96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中圓環(huán)病毒（PCV）表達(dá)                     

QYD124  豬鏈球菌（strep）檢測試劑盒    96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中鏈球菌（strep）表達(dá)                        

QYD125  豬嗜血桿菌(HPS)檢測試劑盒     96孔/盒 用于測定豬血清、血漿及相關(guān)液體樣本中嗜血桿菌(HPS)表達(dá)                         

QYD126  雞禽流感H5N1抗體檢測試劑盒 96孔/盒                             

QYD201  雞禽流感H9抗體檢測試劑盒     96孔/盒                             

QYD201  雞傳染性喉氣管炎抗體檢測試劑盒    96孔/盒                             

QYD201  雞法氏囊抗體檢測試劑盒    96孔/盒                             

QYD201  雞新城疫抗體檢測試劑盒    96孔/盒                             

QYD301  狗狂犬抗體檢測試劑盒       96孔/盒                             

QYD302  旋毛蟲抗體檢測試劑盒       96孔/盒                             

QYD303  犬惡心絲蟲抗體檢測試劑盒       96孔/盒                              【豬白介素1β (IL-1β)ELISA 試劑盒廠家】

QYD401  牛羊亞1型口蹄疫       96孔/盒                             

QYD402  猴B病毒抗體檢測試劑盒(定量）      96孔/盒                             

QYD403  肺吸蟲試劑盒       96孔/盒                             

QYD500  豬藍(lán)耳抗體檢測卡       50T/盒                               

QYD501  豬口蹄疫0型抗體檢測卡    50T/盒                               

QYD502  豬偽狂犬抗體檢測卡    50T/盒                               

QYD503  豬圓環(huán)抗體檢測卡       50T/盒                               

QYD504  豬細(xì)小抗體檢測卡       50T/盒                               

QYD505  豬乙腦抗體檢測卡       50T/盒                               

QYD506  雞禽流感抗體檢測卡    50T/盒                               

QYD507  雞禽流感抗原檢測卡    50T/盒                               

QYD508  H7亞型禽流感抗原快速檢測卡  50T/盒                               

QYD509  H5亞型禽流感抗原快速檢測卡  50T/盒                               

QYD510  H9亞型禽流感抗原快速檢測卡  50T/盒