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豬免疫球蛋白G(IgG)ELISA 試劑盒廠家
點(diǎn)擊次數(shù):16發(fā)布時(shí)間:2016/8/2 21:12:26

更新日期:2016/8/2 21:12:26
所 在 地:中國大陸
產(chǎn)品型號(hào):
優(yōu)質(zhì)供應(yīng)
詳細(xì)內(nèi)容
ELISA酶聯(lián)法試劑盒操作因固相載體的形成不同而有所差異,國內(nèi)醫(yī)學(xué)檢驗(yàn)一般均用板式點(diǎn)。本文將敘述板式ELISA各個(gè)操作步驟的注意要點(diǎn),珠式、管式及磁性球ELSIA,國外試劑均與特殊儀器配合應(yīng)用,兩者均有詳細(xì)的使用說明,嚴(yán)格遵照規(guī)定操作,必能得出準(zhǔn)確的結(jié)果。齊一生物銷售:021-6034 8496;181214 53965;173021 04490
首先齊一生物優(yōu)質(zhì)的試劑盒,良好的儀器和正確的操作是保證ELISA檢測結(jié)果準(zhǔn)確可靠必要條件。所以ELISA試劑盒的購買,試劑質(zhì)量的保證以及實(shí)驗(yàn)前實(shí)驗(yàn)儀器的檢察都是很有重要性的。保證實(shí)驗(yàn)材料的質(zhì)量之后要嚴(yán)格按照實(shí)驗(yàn)步驟進(jìn)行。齊一生物銷售:021-6034 8496;181214 53965;173021 04490
【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】齊一生物ELISA試劑盒
1.精心挑選的進(jìn)口抗體、標(biāo)準(zhǔn)品、顯色劑和酶標(biāo)板全部為進(jìn)口產(chǎn)品實(shí)現(xiàn)*佳性能;
2.原材料批量進(jìn)口加上自配的特殊配方稀釋劑保證高品質(zhì)產(chǎn)品性能的同時(shí)兼顧成本;
3.采用技術(shù),嚴(yán)格按照標(biāo)準(zhǔn)生產(chǎn)方案組裝生產(chǎn);
4.預(yù)包被微孔板的批內(nèi)和批間變異系數(shù)均小于10 %;
5.經(jīng)過嚴(yán)格交叉反應(yīng)和干擾測試及穩(wěn)定性試驗(yàn);
6.明確的敏感度;
7.針對(duì)所有樣品類型達(dá)到*佳的性能;
8.廣泛的檢測范圍可以滿足大多數(shù)檢測要求;
9.完善的售后服務(wù)和免費(fèi)的技術(shù)支持免去您的后顧之憂;
10.強(qiáng)大的生產(chǎn)能力和充足的儲(chǔ)備、操作熟練的技術(shù)人員及高效的生產(chǎn)流程,隨時(shí)可以組裝出您定制的產(chǎn)品。
【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】齊一生物僅供實(shí)驗(yàn)人、大鼠、小鼠、兔等動(dòng)物實(shí)驗(yàn)標(biāo)本檢測使用!得用于人體臨床直接檢測。避免給您帶來不必要的損失,請(qǐng)仔細(xì)閱讀購買說明!齊一生物銷售:021-6034 8496;181214 53965;173021 04490
ELISA試劑盒問題描述
標(biāo)準(zhǔn)曲線梯度差:
吸液或加液不準(zhǔn)請(qǐng)檢查移液器及吸頭
標(biāo)準(zhǔn)品稀釋不正確溶解標(biāo)準(zhǔn)品稍微旋轉(zhuǎn)瓶身,輕輕混勻使粉末完全溶解
洗滌不完全保證洗滌時(shí)間和洗滌次數(shù)及每孔的加液量
顯色很弱或無色:
孵育時(shí)間太短保證充足的孵育時(shí)間
實(shí)驗(yàn)溫度不正確使用推薦的實(shí)驗(yàn)溫度
試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程,保證所有試劑按順序足量添加
讀數(shù)數(shù)值低:
酶標(biāo)儀設(shè)置不正確 在酶標(biāo)儀上檢查波長及濾光片設(shè)置
提前打開酶標(biāo)儀預(yù)熱:變異系數(shù)大加液不正確檢查加液情況
【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】背景值高:
檢測抗體的工作濃度使用推薦的稀釋倍數(shù)
酶標(biāo)板洗滌不完全重新閱讀操作手冊,保證清洗完全;如果用自動(dòng)洗板機(jī),請(qǐng)檢查所有的出口是否有堵塞
洗液有污染配制新鮮的洗液
靈敏度低:
ELISA試劑盒保存按說明書要求保存相關(guān)試劑
讀數(shù)前未終止OD讀數(shù)前應(yīng)在每孔中加入終止液
齊一生物客戶寄標(biāo)本時(shí)需注明以下情況:
1、標(biāo)本編號(hào);2、所測項(xiàng)目;3、是否做復(fù)孔;3、聯(lián)系方式;4、實(shí)驗(yàn)后標(biāo)本是否寄回。
客戶必知:
客戶應(yīng)對(duì)所提供的材料及信息負(fù)責(zé),如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。
試劑盒樣本要求
在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃,必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測,對(duì)收集后當(dāng)天就進(jìn)行檢測的標(biāo)本,及時(shí)儲(chǔ)存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本,請(qǐng)?jiān)炷H〔暮螅瑢?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差,蛋白極易降解,直接影響實(shí)驗(yàn)質(zhì)量,所以避免反復(fù)凍融。具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。齊一生物銷售:021-6034 8496;181214 53965;173021 04490
【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】液體類標(biāo)本:標(biāo)本必須為液體,不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。
1. 血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
2. 血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成應(yīng)再次離心。
3. 尿液、胸腹水、腦脊液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。如有沉淀形成,應(yīng)再次離心。
4. 細(xì)胞培養(yǎng)上清:檢測分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。
5. 培養(yǎng)細(xì)胞:檢測細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融或加入組織蛋白萃取試劑,以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
6. 組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin(抑肽酶)。用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清置于-20度或-70度保存,如有必要,可以將樣品濃縮干燥。分裝后一份待檢測,其余冷凍備用。
7. 標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
8.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
9.如果樣品不能立即檢測,應(yīng)將其分裝,-70 ℃保存,避免反復(fù)冷凍。保存過程中如出現(xiàn)沉淀,應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解
凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
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9238926 Linear guide, BS15 CNY
9238925 Cylinder hinge brass, BS15 CNY
9238922 Tip comb holder, BS15 CNY
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9232320 Cable set, PS 220V, BR8000 CNY
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EK0906 hTLR2-ELISA 人TLR2ELISA試劑盒 Human TLR2 ELISA Kit 96 tests
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EK0928 hTF/F3-ELISA 人組織因子/F3ELISA試劑盒 Human Tissue factor/F3 ELISA Kit 96 tests
EK0309 mBDNF-ELISA 小鼠腦源性神經(jīng)生長因子ELISA試劑盒 Mouse BDNF ELISA Kit 96 tests
EK0313 mBMP-2-ELISA 小鼠BMP-2ELISA試劑盒 Mouse BMP-2 ELISA Kit 96 tests 【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】
EK0326 mEGF-ELISA 小鼠表皮生長因子ELISA試劑盒 Mouse EGF ELISA Kit 96 tests
EK0333 mEPO-ELISA 小鼠促紅細(xì)胞生成素ELISA試劑盒 Mouse EPO ELISA Kit 96 tests
EK0336 mFAS-ELISA 小鼠凋亡相關(guān)因子ELISA試劑盒 Mouse FAS ELISA Kit 96 tests
EK0351 mFN-ELISA 小鼠胚胎纖維連接蛋白ELISA試劑盒 Mouse Fibronectin ELISA Kit 96 tests
EK0361 mG-CSF-ELISA 小鼠粒細(xì)胞集落刺激因子ELISA試劑盒 Mouse G-CSF ELISA Kit 96 tests
EK0365 mGM-CSF-ELISA 小鼠粒細(xì)胞巨噬細(xì)胞刺激因子ELISA試劑盒 Mouse GM-CSF ELISA Kit 96 tests
EK0371 mICAM-1-ELISA 小鼠細(xì)胞間粘附分子ELISA試劑盒 Mouse ICAM-1 ELISA Kit 96 tests
EK0375 mIFN-γ-ELISA 小鼠IFN-γELISA試劑盒 Mouse IFN-γ ELISA Kit 96 tests
EK0378 mIGF-1-ELISA 小鼠IGF-1ELISA試劑盒 Mouse IGF-1 ELISA Kit 96 tests
EK0381 mIGF-2-ELISA 小鼠IGF-2 ELISA試劑盒 Mouse IGF-2 ELISA Kit 96 tests
EK0383 mIGFBP-1-ELISA 小鼠IGFBP-1ELISA試劑盒 Mouse IGFBP-1 ELISA Kit 96 tests
EK0387 mIGFBP-3-ELISA 小鼠IGFBP-3ELISA試劑盒 Mouse IGFBP-3 ELISA Kit 96 tests
EK0391 mIL-1α-ELISA 小鼠IL-1αELISA試劑盒 Mouse IL-1α ELISA Kit 96 tests
EK0394 mIL-1α-ELISA 小鼠IL-1αELISA試劑盒 Mouse IL-1α ELISA Kit 96 tests
EK0398 mIL-2-ELISA 小鼠IL-2ELISA試劑盒 Mouse IL-2 ELISA Kit 96 tests
EK0403 m IL-3 -ELISA 小鼠IL-3ELISA試劑盒 Mouse IL-3 ELISA Kit 96 tests 【豬免疫球蛋白G(IgG)ELISA 試劑盒廠家】
EK0405 m IL-4-ELISA 小鼠IL-4 ELISA試劑盒 Mouse IL-4 ELISA Kit 96 tests
EK0408 m IL-5-ELISA 小鼠IL-5ELISA試劑盒 Mouse IL-5 ELISA Kit 96 tests
EK0411 m IL-6-ELISA 小鼠IL-6ELISA試劑盒 Mouse IL-6 ELISA Kit 96 tests
EK0417 mIL-10-ELISA 小鼠IL-10ELISA試劑盒 Mouse IL-10 ELISA Kit 96 tests
EK0422 mIL-12(p70)-ELISA 小鼠IL-12(p70)ELISA試劑盒 Mouse IL-12(p70) ELISA Kit 96 tests
EK0431 mIL-17-ELISA 小鼠IL-17ELISA試劑盒 Mouse IL-17 ELISA Kit 96 tests
EK0436 mLaminin-ELISA 小鼠層粘連蛋白ELISA試劑盒 Mouse Laminin ELISA Kit 96 tests
EK0438 mLeptin-ELISA 小鼠瘦蛋白ELISA試劑盒 Mouse Leptin ELISA Kit 96 tests
EK0440 mLeptinR-ELISA 小鼠瘦蛋白受體ELISA試劑盒 Mouse Leptin receptor ELISA Kit 96 tests
EK0445 mMCSF-ELISA 小鼠巨噬細(xì)胞集落刺激因子ELISA試劑盒 Mouse M-CSF ELISA Kit 96 tests
EK0460 mMMP-2-ELISA 小鼠MMP-2ELISA試劑盒 Mouse MMP-2 ELISA Kit 96 tests
EK0462 mMMP3-ELISA 小鼠MMP-3ELISA試劑盒 Mouse MMP-3 ELISA Kit 96 tests
n>Calculate:
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
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Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
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EK0642 hTSP2-ELISA 人TSP2ELISA試劑盒 Human TSP2 ELISA Kit 96 tests
EK0644 hCD105-ELISA 人CD105ELISA試劑盒 Human CD105 ELISA Kit 96 tests
EK0658 hAPP-ELISA 人APP ELISA試劑盒 Human APP ELISA Kit 96 tests
EK0663 hBAFF-ELISA 人 BAFF ELISA試劑盒 Human BAFF ELISA Kit 96 tests
EK0667 hPCad-ELISA 人P-CadherinELISA試劑盒 Human P-Cadherin ELISA Kit 96 tests
EK0702 hCD40-ELISA 人CD40ELISA試劑盒 Human CD40 ELISA Kit 96 tests
EK0706 hNCAM-ELISA 人CD56/NCAM-1ELISA試劑盒 Human CD56/NCAM-1 ELISA Kit 96 tests
EK0744 hcMet-ELISA 人c-Met/HGFRELISA試劑盒 Human c-Met/HGFR ELISA Kit 96 tests
EK0756 hErbB2-ELISA 人ErbB-2/Neu2ELISA試劑盒 Human ErbB-2/Neu2 ELISA Kit 96 tests
EK0757 hFeuA-ELISA "人胎球蛋白AELISA試劑盒
" HumanFetuin-A ELISA Kit 96 tests
EK0770 hHBEGF-ELISA 人肝素結(jié)合表皮生長因子ELISA試劑盒 Human HBEGF ELISA Kit 96 tests
EK0777 hIL6Ra-ELISA 人IL-6RaELISA試劑盒 Human IL-6Ra ELISA Kit 96 tests
EK0779 hIL7-ELISA 人IL-7ELISA試劑盒 Human IL-7 ELISA Kit 96 tests 【豬免疫球蛋白M(IgM)ELISA 試劑盒現(xiàn)貨】
EK0799 hIL27-ELISA 人IL-27ELISA試劑盒 Human IL-27 ELISA Kit 96 tests
EK0807 hCSF1R-ELISA 人CSF1R/M-CSFRELISA試劑盒 Human CSF1R/M-CSFR ELISA Kit 96 tests
EK0816 hKal3-ELISA 人Kallikrein 3ELISA試劑盒 Human Kallikrein 3 ELISA Kit 96 tests
EK0829 hRANK-ELISA 人 Receptor Activator of NF-κB (RANK)ELISA試劑盒 Human Receptor Activator of NF-κB (RANK) ELISA Kit 96 tests
EK0850 hMPO-ELISA 人過氧化物酶ELISA試劑盒 Human Myeloperoxidase (MPO) ELISA Kit 96 tests
EK0859 hPAI1-ELISA 人PAI-1ELISA試劑盒 Human PAI-1 ELISA Kit 96 tests
EK0867 hDKK1-ELISA 人DKK-1ELISA試劑盒 Human DKK-1 ELISA Kit 96 tests
EK0873 hPECAM-ELISA 人PECAM-1/CD31ELISA試劑盒 Human PECAM-1/CD31 ELISA Kit 96 tests
EK0884 hBMP7-ELISA 人BMP-7ELISA試劑盒 Human BMP-7 ELISA Kit 96 tests
EK0895 hp53-ELISA 人p53 ELISA試劑盒 Human p53 ELISA Kit 96 tests
EK0904 hCEA-ELISA 人CEAELISA試劑盒 Human CEA ELISA Kit 96 tests