1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9012643  Bottle, system liquid                  CNY                              【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】

9012596  Disposable Tips, 300µl (960)    Conducting disposable tips       CNY                             

133121     NeXtalStock Lithium citrate  (200)                  CNY                             

133119     NeXtalStock Ammonium citrate  (200)           CNY                             

133190     NeXtalStock D-(+)-Glucose (200)               CNY                             

51162       QIAamp 96 DNA Blood Kit (12)        For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels       CNY                             

50112       QIAamp UCP PurePathogen Blood Kit (10)               CNY                             

133104     NeXtalStock Sodium acet.  (200)            CNY                             

9015468  Sensor, photo, PM-L24              CNY                             

9001871  EZ1 Advanced, System     EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

9018458  Screw, w finishing washer (4), QX             CNY                             

9018447  Purge Tool, cartridge, QX                  CNY                             

9012412  Incubator Tower (65°) for Plato 3 hold              CNY                             

9012392  Bottle, waste, 10 l, BR8000              CNY                             

205111     Omniscript RT Kit (50)      For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

9018425  Door, sample, QX              CNY                             

9002123  QIAxcel Advanced System        QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】

57714       QIAamp MinElute Virus Vacuum Kit (50)         For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)      CNY                             

972012     PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16 CNY                             

133227     NeXtalStock Sodium cacodyl. pH 6.0 (100)                CNY                             

301108     PolyFect Transfection Reagent (100 ml)  For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.      CNY                             

56304       QIAamp DNA Micro Kit (50)    For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)     CNY                             

9002031  Rotor-Gene Q MDx 5Plex HRM no PC               CNY                             

9002018  Rotor-Gene Q MDx 2plex HRM, Priority Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

970902     PyroMark Q24 Advanced Kit (4x24)                   CNY                             

133211     NeXtalStock Magnesium chloride H2O (200)            CNY                             

133195     NeXtalStock Sodium tartrate pH 5.6 (200)                CNY                             

293023     mericon Corn (24)             CNY                             

292055     mericon Goat Kit (96)                CNY                             

929521     QX Alignment Marker 15 bp/1 kb (1.5 ml)                 CNY                             

9012598  Disposable Filter-Tips, 1100µl (960)         Conducting disposable filter-tips      CNY                             

206152     QIAGEN Multiplex PCR Plus Kit (100)                CNY                             

QKT0930  水生動物病毒基因組DNA提取試劑盒                                                      

QKT0931  禽流感病毒核酸提取試劑盒                                                       

QKT0932  水生動物病毒基因組DNA/RNA提取試劑盒                                                     

QKT0933  深加工食品DNA提取試劑盒                                                      

QKT0934  動物組織基因組DNA快速提取試劑盒                                                      

QKT0935  細(xì)菌基因組DNA快速提取試劑盒                                                      

QKT0936  結(jié)核分枝桿菌復(fù)合群核酸檢測試劑盒擴增法                                                  

QKT0937  軍團(tuán)菌屬核酸檢測試劑盒熒光法                                                       

QKT0938  嗜肺軍團(tuán)菌核酸檢測試劑盒熒光法                                                   【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】

QKT0939  登革熱病毒DENV I型病毒核酸檢測試劑盒熒光法                                                 

QKT0940  登革熱病毒DENV II型病毒核酸檢測試劑盒熒光法                                                

QKT0941  登革熱病毒DENV III型病毒核酸檢測試劑盒熒光法                                              

QKT0942  羊源性成分核酸檢測試劑盒PCR-熒光探針法                                                  

QKT0943  登革熱病毒DENV IV型病毒核酸檢測試劑盒熒光法                                              

QKT0944  呼吸道21種病原體熒光檢測試劑盒                                                

QKT0945  強化型呼吸系統(tǒng)病原體熒光檢測試劑盒                                                  

QKT0947  細(xì)菌性肺炎熒光檢測試劑盒                                                       

QKT0948  呼吸系統(tǒng)33種病原體熒光檢測試劑盒                                                     

QKT0950  博德氏菌熒光檢測試劑盒                                                  

QKT0951  流感熒光檢測試劑盒                                                  

QKT0952  ACE熒光檢測試劑盒                                                   

QKT0953  巨細(xì)胞病毒熒光檢測試劑盒                                                       

QKT0954  病毒性腸胃炎熒光檢測試劑盒                                                  

QKT0955  細(xì)菌性腸胃炎熒光檢測試劑盒                                                  

QKT0956  腸內(nèi)寄生蟲熒光檢測試劑盒                                                        【豬皮質(zhì)酮/腎上腺酮(CORT)ELISA 試劑盒廠家】

QKT0957  消化系統(tǒng)熒光檢測試劑盒                                                  

QKT0958  艱難梭狀桿菌熒光檢測試劑盒                                                  

QKT0959  丙肝病毒熒光檢測試劑盒                                                  

QKT0960  丙肝病毒熒熒光檢測試劑盒                                                       

QKT0961  皰疹病毒熒光檢測試劑盒                                                  

QKT0962  病毒性腦膜炎熒光檢測試劑盒                                                  

QKT0963  細(xì)菌性腦膜炎熒光檢測試劑盒                                                  

QKT0964  腮腺炎熒光檢測試劑盒