檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

6000-5000        Specimen Collection Tubes                CNY                              【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】

5000-1430        MST Vortexer II - FoamFeetAssembly, 110V             CNY                             

5128-1220        Specimen Transport Medium (STM) CE             CNY                             

5123-1220        Female Swab Specimen Collection KitTM                  CNY                             

5100-1400IVD hc2 Sample Conversion Kit                CNY                             

EA11424  EASYartus BKV RG_II                 CNY                             

5050-1075 V.1 CD Rom Manual, MST Vortexer 2             CNY                             

EA10124  EASYartus EBV RG PCR Kit 2             CNY                             

BR-02       DxS B-RAF Mutation Test Kit            CNY                             

9240729  Tubing, 3/8"OD , Red, AP          CNY                             

9240719  Cable Carrier, Capper, Elevator, AP          CNY                             

9240701  O-Ring, Shaker, Lower Body, AP96/196             CNY                             

9240696  Fitting, Barbed, 1/4" ID tube, AP               CNY                             

9243651  Comprehen Valid., QIAgility     """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

218076     miScript SYBR Green PCR Kit (2000)                  CNY                             

271906     cattletype Milk Prep Kit (50)             CNY                             

289980     intype IC-DNA (1ml)                   CNY                              【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】

280437     virotype BTV RT-PCR Kit (480)          CNY                             

932836     #QIAsymphony Ni-NTA Denat. Kit (384)           CNY                             

800511     Verification Sequencing / DNA production                CNY                             

9238243  ZLV BioRobot M96 User Manual               CNY                             

9019988  ZLV Mainboard, QGY                 CNY                             

9020553  Foam angle, transport box, QIAC              CNY                             

9020582  X-Motor, DML3000           CNY                             

9020584  Y-Belt, DML3000 320 Teeth              CNY                             

9020594  Enclosure, DML3000                 CNY                             

9021006  Motor, Pipettor Y, DCU             CNY                             

9236333  Sample Loop              CNY                             

9013382  Shaker adapter, microplate, LHS              CNY                             

9013275  Motor, gripper, handler T/L, BR3000                CNY                             

9013269  Fitting, PA tubing M6x1, 2.25mm, (10)            CNY                             

9013210  Cabling kit, moving, BR8000             CNY                             

QYS-03149        磷酸果糖激酶PFK測試盒     50管/48樣      紫外分光光度法                                

QYS-03150        磷酸烯醇式丙酮酸羧化酶PEPC測試盒       50管/48樣      紫外分光光度法                                

QYS-03151        丙酮酸PA測試盒   50管/48樣      可見分光光度法                                

QYS-03152        丙酮酸PA測試盒   100管/96樣    酶標(biāo)法                                

QYS-03153        乳酸脫氫酶LDH測試盒         50管/24樣      可見分光光度法                                

QYS-03154        乳酸脫氫酶LDH測試盒         100管/48樣    酶標(biāo)法                                

QYS-03155        酸性蛋白酶測試盒         50管/24樣      可見分光光度法                                

QYS-03156        酸性蛋白酶測試盒         100管/48樣    酶標(biāo)法                                

QYS-03157        中性蛋白酶測試盒         50管/24樣      可見分光光度法                                

QYS-03158        中性蛋白酶測試盒         100管/48樣    酶標(biāo)法                                

QYS-03159        堿性蛋白酶測試盒         50管/24樣      可見分光光度法                                

QYS-03160        堿性蛋白酶測試盒         100管/48樣    酶標(biāo)法                                

QYS-03161        胰蛋白酶測試盒     50管/48樣      紫外分光光度法                                 【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】

QYS-03162        胃蛋白酶測試盒     50管/24樣      可見分光光度法                                

QYS-03163        胃蛋白酶測試盒     100管/48樣    酶標(biāo)法                                

QYS-03164        糜蛋白酶測試盒     50管/48樣      紫外分光光度法                                

QYS-03165        游離脂肪酸含量測試盒         50管/48樣      可見分光光度法                                

QYS-03166        脂肪酸合成酶FAS測試盒      10管/9樣         紫外分光光度法                                

QYS-03167        脂肪酸合成酶FAS測試盒      25管/24樣      紫外分光光度法                                

QYS-03168        脂肪酸合成酶FAS測試盒      50管/48樣      紫外分光光度法                                

QYS-03169        脂肪酶LPS測試盒 50管/48樣      可見分光光度法                                

QYS-03170        醇脫氫酶ADH測試盒   50管/48樣      紫外分光光度法                                

QYS-03171        脂氧合酶LOX測試盒     50管/48樣      紫外分光光度法                                

QYS-03172        �；D(zhuǎn)移酶AAT測試盒         10管/9樣         可見分光光度法                                

QYS-03173        �；D(zhuǎn)移酶AAT測試盒         25管/24樣      可見分光光度法                                

QYS-03174        �；D(zhuǎn)移酶AAT測試盒         50管/48樣      可見分光光度法                                

QYS-03175        丙酮酸脫羧酶PDC測試盒    50管/48樣      紫外分光光度法                                

QYS-03176        脂蛋白酯酶LPL＆肝脂酶HL測試盒    50管/24樣      可見分光光度法                                

QYS-03177        甘油三酯TG含量測試盒       50管/48樣      可見分光光度法                                 【豬可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA 試劑盒廠家】

QYS-03178        血清總膽固醇TC含量測試盒       50管/48樣      可見分光光度法                                

QYS-03179        血清游離膽固醇FC含量測試盒   50管/48樣      可見分光光度法                                

QYS-03180        淀粉含量測試盒     50管/48樣      可見分光光度法                                

QYS-03181        淀粉含量測試盒     100管/96樣    酶標(biāo)法