1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細(xì)步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進(jìn)行階段，我公司客服人員會及時向您匯報實驗進(jìn)程。

9013394  Tubing set , HSPS, 200               CNY                              【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】

9013235  Tubing set,  90/120, vacuum, 0               CNY                             

9013226  Gripper set, L-handler, BR3000                 CNY                             

9013199  Tub.pre.sen.inlet,auto vac,BR8000 v1.00                 CNY                             

9011774  Tip Adapter 1100 ul BR8000             CNY                             

9011709  Dispenser head, BR8000          CNY                             

132559     NeXtal Stock Magnesium acetate (50)    1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)    CNY                            

800309     siRNA Hairpin Run w/o prep             CNY                             

800160     Single Read, w/o prep      "Sequencing of plasmid DNA ａnd PCR products, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

800101     Basic Read Tubes               CNY                             

765512     PAXgene Tissue STABILIZER Conc. (150 ml)              CNY                             

132007     EasyXtal 15-well Tool (100)                CNY                             

201207     Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

9238319  V-drive unit, BR8000, V2          CNY                             

9238178  QIAplate Holder, SBS, black              CNY                             

9238134  Multiwell-Plate Holder, SBS              CNY                             

9240675  Tee, Barbed, Plastic, AP96                CNY                              【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】

9240653  Pressure Pump, Gast, 120V, AP96            CNY                             

9240624  Solid State Relay, AP196/98               CNY                             

9240576  Cable Assy, Capper, X51,CCW, AP96/196           CNY                             

9240546  Motor, Shutter Door, Eppendorf, AP196/98               CNY                             

9240544  Centrifuge, 5810RA, Eppendorf, AP196/98                CNY                             

9240513  Mouse, Standard, AP                 CNY                             

9240508  Software & Std. Protocols, AP98               CNY                             

9240499  Reagent Pump, AP            CNY                             

9240378  Autopure, Installation      The Installation, AUTOPURE facilitates the quick implementation of the AUTOPURE into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the AUTPOURE instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9240242  Connector, disp. tip adapter drive, TR              CNY                             

9239959  EZ1, Premium Agreement       The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9020028  ZB sensor, 320mm, EZ1xl                  CNY                             

9020018  Upgrade kit, vacuum chamber, CAS-1820                 CNY                             

9019987  PCB, small breakout, 8 core, Cxx               CNY                             

9019927  Cover, tip ejector (UV), Cx8               CNY                             

QY-x2449 人宮頸癌腸轉(zhuǎn)移細(xì)胞 Ca Ski 詢價 ATCC                                                  

QY-x2450 大鼠膠質(zhì)瘤細(xì)胞 C6 詢價 ATCC                                                         【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】

QY-x2451 人乳腺管癌細(xì)胞 BT-549 詢價 ATCC                                                

QY-x2452 倉鼠幼倉鼠腎細(xì)胞 BHK-21 [C-13] 詢價 ATCC                                                 

QY-x2453 人肝癌細(xì)胞 BEL-7402 詢價 ATCC                                                  

QY-x2454 小鼠黑色素瘤細(xì)胞 B16 詢價 ATCC                                                 

QY-x2455 人轉(zhuǎn)移胰腺腺癌細(xì)胞 AsPC-1 詢價 ATCC                                                

QY-x2456 人胚腎細(xì)胞 AAV-293 詢價 ATCC                                                      

QY-x2457 人肺癌細(xì)胞 A549 [A-549] 詢價 ATCC                                                       

QY-x2458 人表皮癌細(xì)胞 A-431 詢價 ATCC                                                      

QY-x2459 人T淋巴細(xì)胞白血病細(xì)胞 A3 詢價 ATCC                                                        

QY-x2460 人高轉(zhuǎn)移肺癌細(xì)胞 95-D 詢價 ATCC                                                        

QY-x2461 人腎透明細(xì)胞腺癌細(xì)胞 786-O 詢價 ATCC                                                      

QY-x2462 小鼠脂肪細(xì)胞 3T3-L1 詢價 ATCC                                                     

QY-x2463 人胚腎細(xì)胞 293T 詢價 ATCC                                                   

QY-x2464 人膀胱癌細(xì)胞 5637 詢價 ATCC                                                        

QY-x2465 人胚腎細(xì)胞 293 詢價 ATCC                                                      

QYS-03001        輔酶ⅠNADH含量測試盒      50管/48樣      高效液相色譜法                                 【豬熱休克蛋白90(HSP-90)ELISA 試劑盒現(xiàn)貨】

QYS-03002        輔酶ⅠNADH含量測試盒      50管/48樣      可見分光光度法                                

QYS-03003        輔酶ⅠNADH含量測試盒      100管/96樣    酶標(biāo)法                                

QYS-03004        NAD激酶NADK測試盒 50管/24樣      可見分光光度法                                

QYS-03005        乳酸脫氫酶LDH測試盒         50管/24樣      可見分光光度法                                

QYS-03006        乳酸脫氫酶LDH測試盒         100管/48樣    酶標(biāo)法                                

QYS-03007        蘋果酸脫氫酶MDH測試盒  50管/48樣      紫外分光光度法                                

QYS-03008        線粒體呼吸鏈復(fù)合體Ⅰ活性測試盒     25管/24樣      紫外分光光度法                                

QYS-03009        NADH氧化酶NOX測試盒     50管/48樣      可見分光光度法                                

QYS-03010        檸檬酸合酶CS測試盒   50管/48樣      可見分光光度法                                

QYS-03011        檸檬酸合酶CS測試盒   25管/24樣      可見分光光度法                                

QYS-03012        檸檬酸合酶CS測試盒   10管/9樣         可見分光光度法                                

QYS-03013        丙酮酸脫羧酶PDC測試盒    50管/48樣      紫外分光光度法                                

QYS-03014        醇脫氫酶ADH測試盒   50管/48樣      紫外分光光度法                                

QYS-03015        單脫氫抗壞血酸還原酶MDHAR測試盒      50管/48樣      紫外分光光度法                                

QYS-03016        輔酶ⅡNADPH含量測試盒    50管/48樣      高效液相色譜法