【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

764114    PAXgene Bone Marrow RNA Tubes (50)            CNY                            【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】

679913    ipsogen RT Kit (33)              CNY                           

203643    HotStarTaq Plus Master Mix Kit (250)  "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."     CNY                           

201225    Taq PCR Core Kit (1000)      "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

201203    Taq DNA Polymerase (250)   "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

180922    GeneRead Adapter L Set 1plex (12)             CNY                           

180912    GeneRead Adapter I Set 1plex (12)              CNY                           

159214    QIAcard FTA Indicator Four Spots (25)             CNY                           

159201    QIAcard FTA One Spot (100)              CNY                           

9235523  Needle cleaning assembly P/N 4567-01        CNY                           

9235300  Plunger tip, syringe 2,5ml             CNY                           

9235117  Fuse F4A, v1.00           CNY                           

9022818  EZ1 Advanced XL Flip Cap Tube Rack        CNY                           

9234889  Vacuum manifold, 96 seal             CNY                           

9234862  System liquid container (2l Nalgene)            CNY                           

9239555  PCBA, Laser Driver, Unlimited             CNY                           

9238664  Hinge             CNY                            【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】

9238662  Lid with window           CNY                           

9238659  Cooling compressor T2168GK, 60Hz          CNY                           

9238645  Brake resistor 112 Ohm / 100 W          CNY                           

9242061  Pipetting container, IQ/OQ, QS            CNY                           

9241827  Pyro Q96 ID, Premium Agreement       "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9241166  Board, A/D, QX            CNY                           

9240955  Sensor, z, handler, Qsym              CNY                           

9240835  QIAcube, Basic Agreement, no PM      The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY                           

9240766  32 OUT Module, D2-32TD1, AP96             CNY                           

6000-5018      MST STM Rack & Lid (Blue - 2 pos. cal.)          CNY                           

9241777  QIAxtractor, Inspection Service    "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                           

9241375  Magnet, door lock, top, EZ1a              CNY                           

9241334  Keypad foil, EZ1           CNY                           

9240750  Belt, Elevator, AP           CNY                           

9243648  Acceler. Valid Supp, Other Extract Sys The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).      CNY                           

QY-x2086       HIH 3T3 小鼠胚胎成纖維細(xì)胞 1640+10%胎牛血清                                         

QY-x2087       L929 小鼠成纖維細(xì)胞 1640+10%胎牛血清                                       

QY-x2088       HSC-T6 大鼠肝星狀細(xì)胞 1640+10%胎牛血清                                   

QY-x2089       L-02 人肝細(xì)胞 1640+10%胎牛血清                                    

QY-x2090       SRA01/04 人晶體上皮細(xì)胞 MEM+非必需氨基酸＋10%胎牛血清                                   

QY-x2091       CHO 中國倉鼠卵巢細(xì)胞（野生型） 1640+10%胎牛血清                                        

QY-x2092       IEC-6 大鼠小腸隱窩上皮細(xì)胞 DMEM+10%胎牛血清                                      

QY-x2093       P815 小鼠肥大細(xì)胞 1640+10%胎牛血清                                    

QY-x2094       NRK-49F 大鼠正常腎成纖維細(xì)胞 1640+10%胎牛血清                                    

QY-x2095       自建 正常成人皮膚成纖維細(xì)胞 DMEM+20%胎牛血清 原代培養(yǎng)                                         

QY-x2096       HELF 人胚肺成纖維細(xì)胞 1640+10%胎牛血清 5，6代                                    

QY-x2097       293 人胚腎細(xì)胞 DMEM+10%胎牛血清                                      

QY-x2098       293T 人胚腎T細(xì)胞 1640+10%胎牛血清                                    【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】

QY-x2099       GES-1 人胃粘膜細(xì)胞 DMEM+10%胎牛血清                                     

QY-x2100       3T3-L1 小鼠胚胎成纖維細(xì)胞（前脂肪） DMEM+10%胎牛血清                                    

QY-x2101       Vero 猴腎細(xì)胞 1640+10%胎牛血清                                     

QY-x2102       CHO-dhFr- 中國倉鼠卵巢細(xì)胞(二氫葉酸缺陷型） 1640+10%胎牛血清                                        

QY-x2103       V79 中國倉鼠肺細(xì)胞 1640+10%胎牛血清                                         

QY-x2104       PA317 小鼠胚胎成纖維細(xì)胞 1640+10%胎牛血清                                     

QY-x2105       Cos-7 綠猴腎細(xì)胞 DMEM+10%胎牛血清                                   

QY-x2106       MA104 非洲綠猴胚胎腎上皮細(xì)胞 DMEM+10%胎牛血清                                        

QY-x2107       16HBE 人支氣管上皮細(xì)胞 1640+10%胎牛血清                                        

QY-x2108       Marc145 猴胚胎腎上皮細(xì)胞                                         

QY-x2109       MDA-MB-435S 人乳腺導(dǎo)管癌細(xì)胞                                      

QY-x2110       MDBK 瘤牛腎細(xì)胞                                        

QY-x2111       MEG-01 人成巨核細(xì)胞白血病細(xì)胞                                      

QY-x2112       MFC 小鼠胃癌細(xì)胞                                       

QY-x2113       MG-63 人骨肉瘤細(xì)胞                                    

QY-x2114       MLA144長臂猿淋巴瘤細(xì)胞                                         【豬凋亡相關(guān)因子(FAS/CD95)ELISA 試劑盒廠家】

QY-x2115       MouseM 小鼠肌肉細(xì)胞                                         

QY-x2116       MouseS 小鼠皮膚成纖維樣細(xì)胞                                   

QY-x2117       Hep G2人肝癌細(xì)胞系                                    

QY-x2118       Hep3B人肝癌細(xì)胞系