齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶(hù)在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類(lèi)型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶(hù)都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線(xiàn)。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類(lèi)型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類(lèi)型。未經(jīng)檢驗(yàn)的樣品類(lèi)型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)的值一般是非線(xiàn)性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11。可以使用不同的組件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線(xiàn)看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋?zhuān)?qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶(hù)應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶(hù)決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9018400  Cable, assy, high voltage ON, QX               CNY                              【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

9018395  Key, Service, yellow, QX            CNY                             

9012258  Worktable mount     Adapter for accommodating items with base dimensions of 100 x 150 mm or smaller on the worktable; larger items may be accommodated using multiple adjacent worktable mounts  CNY                             

9012189  Abdeckplatte Nadelantrieb P1xxx            CNY                             

950280     VX Carrier RNA (5 x 1 mg)                  CNY                             

37551       Qproteome Mannose Glycoprotein Kit             CNY                             

9015326  Sensor, photo, EE-SX672A                  CNY                             

239135     GelPilot Mid Range Ladder (100)     Ready-to-use DNA marker, 600µl (100lanes), 6 fragments: 100 – 2000 bp    CNY                             

239125     GelPilot Wide Range Ladder (100)  Ready-to-use DNA marker, 600µl (100lanes), 7 fragments: 200 – 4500 bp    CNY                             

133012     NeXtalStock Ammonium bromide (200)           CNY                             

133010     NeXtalStock ADA untit. (200)           CNY                             

133005     NeXtalStock MES untit. (200)           CNY                             

9011957  SamTrak Rack for twelve 16mm tubes              CNY                             

9011950  Modul, Plate stacker (dark), 10 plates              CNY                             

971510     therascreen® GIST RapidScreen Pyro Kit                   CNY                             

36915       96-Well Magnet       Magnet for separating magnetic beads in wells of 96-well plates, 2 x 96-Well Microplates FB      CNY                             

950097     X-tractor Gene Consumables   The Xtractor Gene Consumables provides fully automated viral nucleic acid purification on the QIAxtractor when used with the Reagent Pack VX (#950207), ａnd gDNA purification when used with the Reagent Pack DX (#950107). The combination of reagents ａnd plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.      CNY                              【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

800405     Library, Small Insert, add＇tl 96 clones                CNY                             

800385     Sequence Comparison               CNY                             

9016529  Transportation box, BioRobot M48          CNY                             

9016525  Transportation box , BR8000/MDx           CNY                             

9016523  Round Carrier Set (4), 10 x 15 ml Falcon           CNY                             

9016517  Plate Holder Set (2)          CNY                             

9016300  Board, X-Main, Immucor, P3141 SI           CNY                             

9016203  Fitting, PCV Tubing, M6x1, 4.75mm (10)                   CNY                             

9016148  #App. Package, M48, Genomic Research                 CNY                             

9016117  Tip-Tray Holder, 10 µl tips        Holder for accommodating a tip tray containing 96 disposable tips of 10 µl capacity    CNY                             

9011914  Manual, user, BR9604, basic, US              CNY                             

9011874  Calibration pyramide, BR9600                   CNY                             

9019146  Cable kit - Power, PyroQ96MD                  CNY                             

9019139  Cable kit - Power, PyroQ96ID            CNY                             

9019132  Gasket - Cartridge, PyroQ96ID                  CNY                             

RND99047        氨基糖苷類(lèi)檢測(cè)試劑盒         Aminoglycosides ELISA Test Kit        96T  美國(guó)REAGEN                    【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

RND99048        強(qiáng)力霉素檢測(cè)試劑盒     Doxycycline  ELISA Test Kit    96T  美國(guó)REAGEN                   

RND99049        土霉素檢測(cè)試劑盒         Oxytetracycline ELISA Test Kit          96T  美國(guó)REAGEN                   

RND99050        金霉素檢測(cè)試劑盒         Chlortetracycline ELISA Test Kit       96T  美國(guó)REAGEN                   

RND99051        喹乙醇檢測(cè)試劑盒         Olaquindox ELISA Test Kit         96T  美國(guó)REAGEN                   

RND99052        紅霉素檢測(cè)試劑盒         Erythromycin ELISA Test Kit     96T  美國(guó)REAGEN                   

RND99055        賽庚啶檢測(cè)試劑盒         Cyproheptadine ELISA Test Kit         96T  美國(guó)REAGEN                   

RND99056        莫能菌素檢測(cè)試劑盒     Monensin ELISA Test Kit  96T  美國(guó)REAGEN                   

RND99058        林可霉素檢測(cè)試劑盒     lincomycin ELISA Test Kit 96T  美國(guó)REAGEN                   

RND99059        地塞米松檢測(cè)試劑盒     Dexamethaone ELISA Test Kit 96T  美國(guó)REAGEN                   

RND99060        頭孢噻呋檢測(cè)試劑盒     Ceftiofur ELISA Test Kit    96T  美國(guó)REAGEN                   

RND99061        尼卡巴嗪檢測(cè)試劑盒     Nicarbazin ELISA Test Kit          96T  美國(guó)REAGEN                   

RND99062        迪美唑檢測(cè)試劑盒         Dimetridazole ELISA Test Kit   96T  美國(guó)REAGEN                   

RND99063        萘啶酸檢測(cè)試劑盒         Nalidixic Acid ELISA Test Kit     96T  美國(guó)REAGEN                   

RND99064        替米考星檢測(cè)試劑盒     Tilmicosin  ELISA Test Kit       96T  美國(guó)REAGEN                   

RND99065        苯扎氯銨檢測(cè)試劑盒     Benzalkonium chloride(BKC) ELISA Test Kit      96T  美國(guó)REAGEN                   

RND99066        喹乙醇代謝物檢測(cè)試劑盒     MQCA ELISA Test Kit        96T  美國(guó)REAGEN                    【兔子葡萄糖依賴(lài)性胰島素釋放多肽(GIP)ELISA試劑盒廠家】

RND99067        溴布特羅檢測(cè)試劑盒     Brombuterol ELISA Test Kit      96T  美國(guó)REAGEN                   

RND99068        惡喹酸檢測(cè)試劑盒         Oxolinic Acid ELISA Test Kit      96T  美國(guó)REAGEN                   

RND99069        多拉菌素檢測(cè)試劑盒     Doramectin ELISA Test Kit       96T  美國(guó)REAGEN                   

RND99070        阿莫西林檢測(cè)試劑盒     Amoxillin ELISA Test Kit   96T  美國(guó)REAGEN                   

RND99071        金剛烷胺檢測(cè)試劑盒     Amantadine ELISA Test Kit       96T  美國(guó)REAGEN                   

RND99072        頭孢哌酮檢測(cè)試劑盒     Cefoperazone ELISA Test Kit    96T  美國(guó)REAGEN                   

RND99073        倍他米松檢測(cè)試劑盒     Betamethaone ELISA Test Kit 96T  美國(guó)REAGEN                   

RND99074        阿苯達(dá)唑檢測(cè)試劑盒     Albendazole ELISA Test Kit       96T  美國(guó)REAGEN                   

RND99075        潑尼松龍檢測(cè)試劑盒     Prednisolone ELISA Test Kit      96T  美國(guó)REAGEN                   

RND99076        甲硝唑檢測(cè)試劑盒         Metronidazole (MNZ) ELISA Test Kit        96T  美國(guó)REAGEN                   

RND99077        桿菌肽檢測(cè)試劑盒         Bacitracin ELISA Test Kit 96T  美國(guó)REAGEN                   

RND99078        雙氟沙星檢測(cè)試劑盒     Difloxacin ELISA Test Kit  96T  美國(guó)REAGEN                   

RND99079        沙拉沙星檢測(cè)試劑盒     Sarafloxacin ELISA Test Kit       96T  美國(guó)REAGEN