【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

291115    mericon RR Soy Kit (96)              CNY                            【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】

291065    mericon GMO Screen bar (96)            CNY                           

9001933  DML 3000 120-240V Q        CNY                           

9001915  PyroMark Q96 ID, Priority    PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

133120    NeXtalStock Tricine (200)           CNY                           

133180    NeXtalStock Citric acid pH 3.0 (200)          CNY                           

9018503  Manual air supply, service tool, QX             CNY                           

51161      QIAamp 96 DNA Blood Kit (4)     For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                           

133106    NeXtalStock Sodium ac. H2O pH 4.6 (200)        CNY                           

9015519  Spring, for door            CNY                           

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority     QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001875  EZ1 Advanced XL, Priority    EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

959236    MagAttract RNA Tissue Mini M48 Kit (192)        CNY                           

956336    MagAttract RNA Univ. Tissue M48 Kit(192)        CNY                           

9018457  Cartridge, alignment tool, QX              CNY                           

9015467  Motor, P         CNY                           

9015451  Modules, DISU, BR8000 v2.1              CNY                            【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】

9012427  Thermostate system, complete, 120V, BR30        CNY                           

9018483  Notebook, QX              CNY                           

51297     FlexiGene DNA AGF3000 Kit (640)      "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"   CNY                           

51185      QIAamp DNA Blood Midi Kit (100)      For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) CNY                           

38075     AIL Cartridge (5ml)              CNY                           

9015471  Sensor, photo, PM-U24         CNY                           

956034    EZ1 RNA Universal Tissue Kit (48)      Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.      CNY                           

204772    Rotor-Gene Multiplex PCR Kit (80)      The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.  CNY                           

9018435  Tubing, external N2 source, 10ft, QX          CNY                           

9018417  Cover, front, blue window, QX            CNY                           

9018403  ZLV, Detect barrel (1), QX           CNY                           

290103    mericon Shigella spp Kit (24)        CNY                           

9015392  Clamp, rubber, adapter plate, HS shaker,             CNY                           

9001793  QIAcube HT/QIAxtractor, system        CNY                           

9012330  Syringe Pump outlet tubing           CNY                           

QKT0574       總膽紅素測定試劑盒重氮化法                                      

QKT0575       ａ-L-巖藻糖苷酶AFU檢測試劑盒CNPF法                                       

QKT0576       甘油三酯TG檢測試劑盒酶比色法                                      

QKT0577       低密度脂蛋白膽固醇LDL檢測試劑盒酶直接法                                        

QKT0578       載脂蛋白aLpa檢測試劑盒比濁法                                         【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】

QKT0579       膽固醇CHOL檢測試劑盒酶比色法                                      

QKT0580       乳酸脫氫酶LDH檢測試劑盒連續(xù)監(jiān)測法                                    

QKT0581       肌酐CREA檢測試劑盒PAP肌氨酸氧化酶法                                     

QKT0582       丙型肝炎病毒抗體HCV檢測試劑盒膠體金法                                    

QKT0584       嗎啡檢測試劑MOP檢測試劑盒膠體金法                                    

QKT0585       總膽紅素測定試劑盒重氮化法                                      

QKT0586       丙型肝炎病毒抗體診斷試劑盒酶聯(lián)免疫法                                         

QKT0587       甲型肝炎病毒IgM抗體診斷試劑盒酶聯(lián)免疫法                                         

QKT0588       梅毒甲苯胺紅不加熱血清試驗診斷試劑                                      

QKT0589       人類免疫缺陷病毒抗體診斷試劑盒酶聯(lián)免疫法                                         

QKT0590       乙肝兩對半檢測試劑盒酶聯(lián)免疫法                                      

QKT0591       乙型肝炎病毒核心抗體IgM診斷試劑盒酶聯(lián)免疫法                                         

QKT0592       嗎啡檢測試劑MOP檢測試劑盒膠體金法                                    

QKT0593       ａ-L-巖藻糖苷酶AFU檢測試劑盒CNPF法                                       

QKT0594       甘油三酯TG檢測試劑盒酶比色法                                      

QKT0595       低密度脂蛋白膽固醇LDL檢測試劑盒酶直接法                                         【兔熱休克蛋白90(HSP-90)ELISA試劑盒廠家】

QKT0596       載脂蛋白aLpa檢測試劑盒比濁法                                        

QKT0597       抗環(huán)瓜氨酸肽抗體測定試劑盒膠乳增強免疫比濁法                                         

QKT0598       脂肪酶測定試劑盒LPS       酶比色法                                  

QKT0599       β-羥丁酸測定試劑盒β-HB       酶法                                  

QKT0600       二氧化碳測定試劑盒CO2   酶法                                  

QKT0601       鋅測定試劑盒Zn   比色法銅測定試劑盒                               

QKT0602       缺血修飾白蛋白測定試劑盒IMA       白蛋白-鈷結(jié)合試驗法                             

QKT0603       C反應(yīng)蛋白測定試劑盒CRP       膠乳增強免疫比濁法                               

QKT0604       鐵測定試劑盒Fe   比色法                               

QKT0570       同型半胱氨酸檢測試劑盒HCY酶法                                    

QKT0571       胱抑素CCys-C檢測試劑盒免疫比濁法                                       

QKT0572       糖化血紅蛋白HbA1c檢測試劑盒酶法                                        

QKT0573       二氧化碳CO2檢測試劑盒酶法