價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過(guò)程非常重要，不充分的洗滌易造成假陽(yáng)性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高，請(qǐng)先稀釋后再測(cè)定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測(cè)溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9243701  careHPV, Training             CNY                              【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

EA11423  EASYartus BKV RG_I                  CNY                             

800190     Sequencing in a Box Service              CNY                             

800110     Single Read, w/ prep        "Bacterial clone cultivation ａnd template purification or PCR product purification, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

132538     NeXtal Stock Dioxane (50)        100% v/v Dioxane - 50mL (5 x 10 ml tube)       CNY                             

338162     RT2 PCR Array Loading Reservoir             CNY                             

338114     Mouse XpressRef Universal Total RNA              CNY                             

203743     QIAGEN Fast Cycling PCR Kit (200)  For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution CNY                             

180820     GeneRead qPCR SYBR Green MM (2)               CNY                             

159320     Desicant (1000)                 CNY                             

159142     QIAsafe DNA Blood 48-Well Plates (10)            CNY                             

158822     Gentra Puregene Buccal Cell Kit (10)                CNY                             

158689     Gentra Puregene Tissue Kit (33 g)            CNY                             

678691     ipsogen MLL-AF9 type A e10e6 Standards                CNY                             

32943       cis-Repressed pQE-Kan Vector Set            CNY                             

32506       EasyXpress Protein Synthesis Maxi Kit     For reactions up to 4000 ul: 4 x 350 ul E. coli extract, reaction buffer, RNase-free water, ａnd positive-control DNA     CNY                             

30930       Glutathione HiCap Matrix (100 ml)          CNY                              【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

30725       #Ni-NTA Superflow Cartridges (100x1 ml)                 CNY                             

30210       Ni-NTA Agarose (25 ml)   25 ml nickel-charged resin (max. pressure: 2.8 psi)          CNY                             

28183       QIAquick 96 PCR Purification Kit (24)       For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps        CNY                             

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY                             

9013170  Tubing, syringe inlet 2, BR8000                 CNY                             

9011417  Klemmplättchen Varispan P7 (Alodine)             CNY                             

5050-1020EN   #Keyboard English Spare          CNY                             

9240731  Tubing, 3/8"OD , Blue, AP                   CNY                             

676091     ipsogen CBFB-MYH11 A Standards           CNY                             

9011341  Microplate Washer Module Plato 3                   CNY                             

9011203  Vacuum control unit, BR8000           CNY                             

673522     ipsogen JAK2 MutaQuant Kit (12) CE                 CNY                             

337839     qBiomarker SYBR Fluor Mastermix 25 ml                  CNY                             

337632     qBiomarker SYBR Green ROX Mastermix (12)                   CNY                             

QY-x1774 人CD4+T淋巴細(xì)胞         H9                                        

QY-x1775 胎兒骨髓間充質(zhì)細(xì)胞（自建）     FBM                                     

QY-x1776 　     人腦細(xì)胞                                              

QY-x1777 人腦膠質(zhì)細(xì)胞株     HEB                                      

QY-x1778 總T淋巴細(xì)胞          OKT3                                              【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

QY-x1779 BALB/C小鼠肝癌細(xì)胞    H22                                      

QY-x1780 小鼠前列腺癌細(xì)胞         RM-1                                             

QY-x1781 大鼠肝癌細(xì)胞（wistar 大鼠）     CBRH7919                                            

QY-x1782 小鼠肝癌細(xì)胞         HePa1-6                                       

QY-x1783 小鼠結(jié)腸癌     C26                                       

QY-x1784 穩(wěn)定轉(zhuǎn)染了neor和LIF基因的STO細(xì)胞株 SNL                                       

QY-x1785 C57BL/6小鼠纖維肉瘤細(xì)胞   S180                                              

QY-x1786 小鼠艾氏腹水瘤細(xì)胞     ECA                                      

QY-x1787 大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞         PC-12                                            

QY-x1788 大鼠膠質(zhì)瘤     C6                                         

QY-x1789 褐鼠胰島素瘤上皮細(xì)胞         RIN-m                                           

QY-x1790 小鼠肥大細(xì)胞瘤細(xì)胞     P815                                              

QY-x1791 小鼠雜交瘤細(xì)胞     ST2/o                                             

QY-x1792 小鼠白血病細(xì)胞     P388                                              

QY-x1793 小鼠腎癌細(xì)胞         RenCa                                           

QY-x1794 小鼠胰島素瘤β細(xì)胞（NOD/Lt轉(zhuǎn)基因小鼠，SV40巨T抗原）       NIT-1                                              【兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒現(xiàn)貨】

QY-x1795 大鼠正常肝細(xì)胞     BRL                                      

QY-x1796 小鼠單核巨噬細(xì)胞         RAW 264.7                                            

QY-x1797 小鼠成纖維細(xì)胞     L929                                              

QY-x1798 BALBA小鼠成纖維細(xì)胞          BALB—3T3                                           

QY-x1799 早代小鼠胚胎成纖維細(xì)胞     MEF                                     

QY-x1800 中國(guó)倉(cāng)鼠肺細(xì)胞     V79                                      

QY-x1801 非洲綠猴腎細(xì)胞     Vero                                     

QY-x1802 非洲綠猴腎細(xì)胞     Vero-E6                                        

QY-x1803 恒河猴腎細(xì)胞（中國(guó)預(yù)防醫(yī)學(xué)科學(xué)院病毒研究所惠贈(zèng)）         MA-104