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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購(gòu)買(mǎi)任意ELISA試劑盒都享受免費(fèi)代測(cè)服務(wù)，更多進(jìn)口原裝品牌試劑盒供應(yīng)齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】酶法檢測(cè)試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說(shuō)明書(shū)儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開(kāi)蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】齊一生物客戶寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號(hào)；2、所測(cè)項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對(duì)所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)，以上資料供參考如有疑問(wèn)需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9013315  Motor, handler T/L, BR3000             CNY                              【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】

9013262  Cable, 24 V, power to power, 1200 mm           CNY                             

9013249  BGR Wash System             CNY                             

9013207  Service kit, accessories, BR8000               CNY                             

9013194  Tubing, L0 outlet, auto vac,BR8000 v1.00                CNY                             

9013187  Holder, retractable tubing, dispenser, B          CNY                             

800328     Miniprep, PCR purification (Easy-Read)            CNY                             

132109     EasyXtal 15-Well DG-Tool X-Seal (100)     "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."    CNY                            

132101     NeXtal DWBlock Adhesive Foils                 CNY                             

132045     NeXtal Evolution µplate (10)             CNY                             

338172     RT2 Calibration Plate A             CNY                             

338125     384EZload Covers (4)                 CNY                             

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                              【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                             

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                            

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

QY-x1654 C57小鼠黑色素瘤瘤株ME                                                 

QY-x1655 KM小鼠未分化神經(jīng)膠質(zhì)瘤瘤株B22                                                 

QY-x1656 小鼠Lewis肺癌瘤株LLT                                                      

QY-x1657 615小鼠前胃癌瘤株Fc                                               

QY-x1658 小鼠乳腺癌細(xì)胞株EMT-6                                                   

QY-x1659 小鼠腎癌細(xì)胞株RuCa                                                 

QY-x1660 大鼠肉瘤細(xì)胞WK256                                                  

QY-x1661 小鼠乳腺癌細(xì)胞MA891                                                      

2QY-x1662        大鼠胰腺癌細(xì)胞株DSL-6A/C1                                                     

QY-x1663 小鼠骨髓瘤細(xì)胞NS1                                                   

QY-x1664 大鼠卵巢癌細(xì)胞株NUTU-19                                                       

QY-x1665 小鼠腎癌細(xì)胞Ranca                                                    

QY-x1666 小鼠胚胎成纖維細(xì)胞3T3-swiss albino                                                【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】

QY-x1667 小鼠胚胎成纖維細(xì)胞3T3 clone A31                                                   

QY-x1668 小鼠胰腺腺泡細(xì)胞癌細(xì)胞MPC-83                                                     

QY-x1669 小鼠白血病細(xì)胞C3                                                      

QY-x1670 小鼠β胰島素瘤細(xì)胞RIN-m5F                                                    

QY-x1671 小鼠自發(fā)高乳腺癌細(xì)胞TA2                                               

QY-x1672 小鼠淋巴瘤細(xì)胞Nb2－11                                                   

QY-x1673 小鼠骨髓瘤細(xì)胞NS-1                                                  

QY-x1674 小鼠腎癌細(xì)胞Kert-3                                                    

QY-x1675 HPVl6 E6、E7和ras基因共轉(zhuǎn)化的C57BL／C(H-2b)小鼠肺上皮細(xì)胞TC-1                                                      

QY-x1676 長(zhǎng)臂猿淋巴瘤MLA-144                                              

QY-x1677 細(xì)胞名稱         細(xì)胞代號(hào)         ATCC號(hào)碼        組織來(lái)源         培養(yǎng)基     ATCC描述/中科院細(xì)胞庫(kù)描述      

QY-x1678 人胚腎細(xì)胞     293T         CRL-11268?     腎     DMEM＋10％FBS            

QY-x1679 人腎上皮細(xì)胞系     293            腎     DMEM＋10％FBS            

QY-x1680 人腎上皮細(xì)胞系     293H                  腎     DMEM＋10％FBS            

QY-x1681 人臍靜脈內(nèi)皮細(xì)胞         HUVEC               臍帶靜脈         ECM或             

QY-x1682 EGM-2                                                    【兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒現(xiàn)貨】

QY-x1683 人胚肺成纖維細(xì)胞         HLF  胚胎；肺         MEM α(+)+10%FBS