1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強(qiáng),重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

9240692 Fan, 12Vdc, PC-104 cover, AP196/98      CNY             【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】

51331   QIAamp 96 DNA QIAcube HT Kit (5)        CNY            

74171   RNeasy 96 QIAcube HT Kit (5)        CNY            

180994  GeneRead Adapter L Set 12-plex (72)     CNY            

9002140 careHPV test Luminometer        CNY            

9230355 Washstation P1xxx       CNY            

9235295 Sound absorber (muffler)        CNY            

9235277 Board, HS shaker, BR8000        CNY            

9230202 Safety shield for size1     CNY            

9234858 Toubing coupling piece (washer tubing)      CNY            

9238675 Operating mechanism,  left mech. lock       CNY            

9238655 Computer board cable, 3-channel     CNY            

9238633 Grey wedge (long)   " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor.

SIGMA sensor"   CNY            

9238622 Main board Zent3, no Eprom (>SN85353)       CNY            

9241891 QIArent&go - Rent out fee Rotor-Gene        CNY            

9241823 Pyro Q96 ID, Full Agreement The Full Cover Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.   CNY            

9241816 Pyro Q96 MD, PM Agreement       CNY             【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】

9241807 Pyro Q96 ID, Installation   Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements    CNY            

9241125 Thermal Joint Compound      CNY            

9240978 PCB, connector, sample tracking, Qsym       CNY            

9240947 Encoder, x, portal, Qsym        CNY            

9234821 Manual, user, QS 4.0, US        CNY            

9240785 Reagent #1 Manifold Update, AP98        CNY            

9241784 QIAxcel, Training   "The Training, QIAxcel is a 2 days on-site service for up to four QIAxcel users, conducted by a QIAGEN Field Service Specialist. It includes an introduction to operation of the QIAxcel system hardware ａnd the usage of the BioCalculator software. It covers the preparation of sample material, run preparation, data acquisition, ａnd data evaluation. The training is performed using standardized sample material; customer samples may be processed upon request.

All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. Training certificates for participants can be issued upon request." CNY            

9243544 Autopure, PM Service    "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9242713 Add. Unit, Validation Support, 2d   Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Guided or Accelerated validation service catalog no(s). Scope of work defined from the primary unit utilized in "Guided Validation Support" or "Accelerated Validation Support".  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.    CNY            

9242545 Quick Disconnect O-ring set, LC     CNY            

9242337 Tool, calibration, AS, Qsym     CNY            

5000-1303   Potentiometer Speed, SKR1       CNY            

5000-1283   DML 2000 - Power Cord, 220V B Type (IT)     CNY            

5000-1279   Led Assembly, DML2      CNY            

5000-1269   Optical Sensor New STY, DML2        CNY            

QY-x1364    人黑色素瘤細(xì)胞SK37                     

QY-x1365    人黑色素瘤細(xì)胞A875                     

QY-x1366    人皮膚黑色素瘤細(xì)胞SK-MEL-1                     

QY-x1367    人惡性黑色素瘤細(xì)胞A375                     

QY-x1368    人侵襲性脈絡(luò)膜黑色素瘤細(xì)胞M619                     

QY-x1369    人黑色素瘤細(xì)胞 M14                     

QY-x1370    人葡萄膜黑色素細(xì)胞UM                        【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】

QY-x1371    人視網(wǎng)膜色素上皮細(xì)胞D407                       

QY-x1372    人視網(wǎng)膜母細(xì)胞瘤Rb                     

QY-x1373    人眼脈絡(luò)黑色素瘤細(xì)胞OCM-1                      

QY-x1374    人眼脈絡(luò)黑色素瘤細(xì)胞MuM－2C                    

QY-x1375    人眼脈絡(luò)黑色素瘤細(xì)胞MuM－2B                    

QY-x1376    人低侵襲性脈絡(luò)膜黑色素素瘤細(xì)胞OCM-1A                       

QY-x1377    人視網(wǎng)膜母細(xì)胞瘤Y79                    

QY-x1378    熒光素酶轉(zhuǎn)染人成骨肉瘤細(xì)胞U2OS-Luc teT-on                      

QY-x1379    人橫紋肌肉瘤細(xì)胞TE671 subline No.2                     

QY-x1340    人眼脈絡(luò)黑色素瘤細(xì)胞C918                       

QY-x1341    人橫紋癌細(xì)胞A673                       

QY-x1342    人惡性胚胎橫紋肌瘤細(xì)胞RD                       

QY-x1343    人表皮癌細(xì)胞A-431                      

QY-x1344    人正常皮膚細(xì)胞 HaCaT                       

QY-x1345    人皮膚纖維微細(xì)胞系Malmc                    

QY-x1346    人皮膚成纖維細(xì)胞系BJ                        【兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒廠家】

QY-x1347    人成纖維肉瘤細(xì)胞HT1080                     

QY-x1348    人皮膚癌細(xì)胞系HS-1                     

QY-x1349    人皮膚癌細(xì)胞系HS-4                     

QY-x1350    人胸膜瘤細(xì)胞SMC-1                      

QY-x1351    人胸腺激酶缺陷性細(xì)胞系 143TK                       

QY-x1352    人舌癌細(xì)胞Tca-8113                     

QY-x1353    人舌癌細(xì)胞系Tca8113-P60