ELISA酶聯(lián)法試劑盒操作因固相載體的形成不同而有所差異，國內(nèi)醫(yī)學(xué)檢驗(yàn)一般均用板式點(diǎn)。本文將敘述板式ELISA各個(gè)操作步驟的注意要點(diǎn)，珠式、管式及磁性球ELSIA，國外試劑均與特殊儀器配合應(yīng)用，兩者均有詳細(xì)的使用說明，嚴(yán)格遵照規(guī)定操作，必能得出準(zhǔn)確的結(jié)果。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

首先齊一生物優(yōu)質(zhì)的試劑盒，良好的儀器和正確的操作是保證ELISA檢測結(jié)果準(zhǔn)確可靠必要條件。所以ELISA試劑盒的購買，試劑質(zhì)量的保證以及實(shí)驗(yàn)前實(shí)驗(yàn)儀器的檢察都是很有重要性的。保證實(shí)驗(yàn)材料的質(zhì)量之后要嚴(yán)格按照實(shí)驗(yàn)步驟進(jìn)行。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】齊一生物ELISA試劑盒

1.精心挑選的進(jìn)口抗體、標(biāo)準(zhǔn)品、顯色劑和酶標(biāo)板全部為進(jìn)口產(chǎn)品實(shí)現(xiàn)*佳性能；

2.原材料批量進(jìn)口加上自配的特殊配方稀釋劑保證高品質(zhì)產(chǎn)品性能的同時(shí)兼顧成本；

3.采用技術(shù)，嚴(yán)格按照標(biāo)準(zhǔn)生產(chǎn)方案組裝生產(chǎn)；

4.預(yù)包被微孔板的批內(nèi)和批間變異系數(shù)均小于10 ％；

5.經(jīng)過嚴(yán)格交叉反應(yīng)和干擾測試及穩(wěn)定性試驗(yàn)；

6.明確的敏感度；

7.針對所有樣品類型達(dá)到*佳的性能；

8.廣泛的檢測范圍可以滿足大多數(shù)檢測要求；

9.完善的售后服務(wù)和免費(fèi)的技術(shù)支持免去您的后顧之憂；

10.強(qiáng)大的生產(chǎn)能力和充足的儲備、操作熟練的技術(shù)人員及高效的生產(chǎn)流程，隨時(shí)可以組裝出您定制的產(chǎn)品。

【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】齊一生物僅供實(shí)驗(yàn)人、大鼠、小鼠、兔等動物實(shí)驗(yàn)標(biāo)本檢測使用！得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！齊一生物銷售：021－6034 8496；181214 53965；173021 04490

ELISA試劑盒問題描述

標(biāo)準(zhǔn)曲線梯度差：

吸液或加液不準(zhǔn)請檢查移液器及吸頭

標(biāo)準(zhǔn)品稀釋不正確溶解標(biāo)準(zhǔn)品稍微旋轉(zhuǎn)瓶身，輕輕混勻使粉末完全溶解

洗滌不完全保證洗滌時(shí)間和洗滌次數(shù)及每孔的加液量

顯色很弱或無色：

孵育時(shí)間太短保證充足的孵育時(shí)間

實(shí)驗(yàn)溫度不正確使用推薦的實(shí)驗(yàn)溫度

試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程，保證所有試劑按順序足量添加

讀數(shù)數(shù)值低：

酶標(biāo)儀設(shè)置不正確 在酶標(biāo)儀上檢查波長及濾光片設(shè)置

提前打開酶標(biāo)儀預(yù)熱：變異系數(shù)大加液不正確檢查加液情況

【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】背景值高：

檢測抗體的工作濃度使用推薦的稀釋倍數(shù)

酶標(biāo)板洗滌不完全重新閱讀操作手冊，保證清洗完全；如果用自動洗板機(jī)，請檢查所有的出口是否有堵塞

洗液有污染配制新鮮的洗液

靈敏度低：

ELISA試劑盒保存按說明書要求保存相關(guān)試劑

讀數(shù)前未終止OD讀數(shù)前應(yīng)在每孔中加入終止液

齊一生物客戶寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號；2、所測項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

試劑盒樣本要求

在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。具體操作注意事項(xiàng)請與我司技術(shù)人員溝通。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

1. 血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

2. 血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成應(yīng)再次離心。

3. 尿液、胸腹水、腦脊液：用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。

4. 細(xì)胞培養(yǎng)上清：檢測分泌性的成份時(shí)，用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。

5. 培養(yǎng)細(xì)胞:檢測細(xì)胞內(nèi)的成份時(shí)，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融或加入組織蛋白萃取試劑，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

6. 組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin（抑肽酶）。用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清置于-20度或-70度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。

7. 標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融

8.不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。

9.如果樣品不能立即檢測，應(yīng)將其分裝，-70 ℃保存，避免反復(fù)冷凍。保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解

凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

QY-x1384       小鼠肺癌細(xì)胞LLC                                         

QY-x1385       小鼠胃癌細(xì)胞MFC                                        

QY-x1386       小鼠艾氏腹水癌細(xì)胞EAC                                     

QY-x1387       小鼠腹水瘤細(xì)胞SAC-II B2                                    

QY-x1388       小鼠腹水瘤細(xì)胞SAC-II C3                                    

QY-x1389       小鼠乳腺癌高轉(zhuǎn)移細(xì)胞MA-891                                    

QY-x1390       小鼠乳腺腫瘤細(xì)胞C127                                        

QY-x1391       大鼠乳腺癌細(xì)胞SHZ-88                                        

QY-x1392       小鼠骨髓瘤細(xì)胞SP2/0                                   

QY-x1393       鼠骨髓瘤細(xì)胞SP2/0-Ag14                                     

QY-x1394       小鼠骨髓瘤細(xì)胞P3-X63-Ag8.653                                          【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】

QY-x1395       小鼠骨髓瘤細(xì)胞P3/NSI/1-Ag4-1[NS-1]                                       

QY-x1396       小鼠骨髓瘤細(xì)胞FO                                       

QY-x1397       小鼠淋巴瘤細(xì)胞Yac-1                                    

QY-x1398       小鼠淋巴瘤細(xì)胞EL4                                      

QY-x1399       小鼠淋巴瘤細(xì)胞EL4.IL-2                                      

QY-x1400       小鼠黑色素瘤細(xì)胞B16F1                                      

QY-x1401       鼠黑色素瘤細(xì)胞系B16-Fo                                     

QY-x1402       小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞B16F10                                        

QY-x1403       小鼠黑色素瘤細(xì)胞B16BL6                                    

QY-x1404       小鼠黑色素瘤細(xì)胞B16                                   

QY-x1405       小鼠SRSV轉(zhuǎn)化的3T3細(xì)胞SRSV/3T3                                       

QY-x1406       小鼠胚胎成纖維細(xì)胞BALB/3T3 clone A31                                   

QY-x1407       小鼠肝癌細(xì)胞H22                                   

QY-x1408       小鼠肝癌細(xì)胞Hepa 1-6                                   

QY-x1409       大鼠肝癌細(xì)胞CBRH-7919                                     

QY-x1410       大鼠肝癌細(xì)胞RH-35                                       【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】

QY-x1411       小鼠畸胎瘤細(xì)胞F9                                        

QY-x1412       小鼠畸胎瘤細(xì)胞P19                                      

QY-x1413       大鼠神經(jīng)膠質(zhì)瘤細(xì)胞C6                                        

9235295  Sound absorber (muffler)             CNY                           

9235277  Board, HS shaker, BR8000           CNY                           

9230202  Safety shield for size1           CNY                           

9234858  Toubing coupling piece (washer tubing)              CNY                           

9238675  Operating mechanism,  left mech. lock              CNY                           

9238655  Computer board cable, 3-channel         CNY                           

9238633  Grey wedge (long) " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor.

SIGMA sensor"      CNY                           

9238622  Main board Zent3, no Eprom (>SN85353)          CNY                           

9241891  QIArent&go - Rent out fee Rotor-Gene              CNY                           

9241823  Pyro Q96 ID, Full Agreement       The Full Cover Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.       CNY                           

9241816  Pyro Q96 MD, PM Agreement             CNY                            【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】

9241807  Pyro Q96 ID, Installation      Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements       CNY                           

9241125  Thermal Joint Compound             CNY                           

9240978  PCB, connector, sample tracking, Qsym             CNY                           

9240947  Encoder, x, portal, Qsym             CNY                           

9234821  Manual, user, QS 4.0, US             CNY                           

9240785  Reagent #1 Manifold Update, AP98              CNY                           

9241784  QIAxcel, Training   "The Training, QIAxcel is a 2 days on-site service for up to four QIAxcel users, conducted by a QIAGEN Field Service Specialist. It includes an introduction to operation of the QIAxcel system hardware ａnd the usage of the BioCalculator software. It covers the preparation of sample material, run preparation, data acquisition, ａnd data evaluation. The training is performed using standardized sample material; customer samples may be processed upon request.

All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. Training certificates for participants can be issued upon request."     CNY                           

9243544  Autopure, PM Service    "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9242713  Add. Unit, Validation Support, 2d   Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Guided or Accelerated validation service catalog no(s). Scope of work defined from the primary unit utilized in "Guided Validation Support" or "Accelerated Validation Support".  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.   CNY                           

9242545  Quick Disconnect O-ring set, LC         CNY                           

9242337  Tool, calibration, AS, Qsym          CNY                           

5000-1303      Potentiometer Speed, SKR1          CNY                           

5000-1283      DML 2000 - Power Cord, 220V B Type (IT)              CNY                           

5000-1279      Led Assembly, DML2           CNY                           

5000-1269      Optical Sensor New STY, DML2         CNY                           

5000-1267      Power Transformer, DML2          CNY                            【山羊生長激素釋放多肽(GHRP)ELISA試劑盒現(xiàn)貨】

5000-1263      PMT Housing W IRIS PCB, DML2             CNY                           

5050-1037      Microplate Heater Interface Cable         CNY                           

5025-1022      Expand 4 Power Supply (English)        CNY                           

6000-4114EN  RCS Tech Reference Guide (English)          CNY