產(chǎn)品展示
馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家
點擊次數(shù):23發(fā)布時間:2016/7/30 19:53:20

更新日期:2016/7/30 19:53:20
所 在 地:中國大陸
產(chǎn)品型號:
優(yōu)質(zhì)供應(yīng)
詳細內(nèi)容
【友情提示】:本產(chǎn)品僅供科研研究使用,不得用于人體臨床直接檢測。避免給您帶來不必要的損失,請仔細閱讀購買說明!
【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】酶法檢測試驗所需自備物品:
1. 酶標儀(450nm 波長濾光片)
2. 高精度移液器,EP 管及一次性吸頭
3. 37℃恒溫箱,雙蒸水或去離子水
4. 吸水紙
試劑盒安全性提示:
1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2.實驗中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份。
【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】操作注意事項:
1.試劑盒應(yīng)按標簽說明書儲存,使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標準品應(yīng)丟棄,不可保存。
2.實驗中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。
3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質(zhì)前使用。
4.使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時,避免使用帶金屬部分的加樣器。
5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6.洗滌酶標板時應(yīng)充分拍干,不要將吸水紙直接放入酶標反應(yīng)孔中吸水。
7.底物A應(yīng)揮發(fā),避免長時間打開蓋子。底物B對光敏感,避免長時間暴露于光下。避免用手接觸,有毒。實驗完成后應(yīng)立即讀取OD值。
8.加入試劑的順序應(yīng)一致,以保證所有反應(yīng)板孔溫育的時間一樣。
9.按照說明書中標明的時間、加液的量及順序進行溫育操作。
【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】齊一生物客戶寄標本時需注明以下情況:
1、標本編號;2、所測項目;3、是否做復(fù)孔;3、聯(lián)系方式;4、實驗后標本是否寄回。
客戶必知:
客戶應(yīng)對所提供的材料及信息負責,如因客戶提供的材料及信息不準確而引起的實驗延誤或經(jīng)濟損失由客戶承擔。
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性,在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。
樣本保存條件:
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個月
3.-50℃以下的冰箱,可以放一年
樣本收集
1.血清樣本用干燥管(紅色)或者促凝管(黃色)收集
2.血漿用抗凝管,一般有EDTA抗凝(紫色)、枸櫞酸鈉抗凝(黑色或者藍色)
3.動物組織勻漿(細胞):
1g組織加入9ml PBS(濃度為0.1M,pH為7.4的)測分泌型指標
1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)
糞便樣本:1g樣本+9ml PBS(濃度為0.01M,pH為7.4的)
沒有PBS的話也可以用一下生理鹽水,還是用PBS
樣本離心轉(zhuǎn)速及時間
1.試管采集的樣本:3000轉(zhuǎn),離心五分鐘取上清
2.離心管或者EP管采集的樣本:5000--8000轉(zhuǎn)3分鐘取上清
3..樣本保存--如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】齊一生物供應(yīng),以上資料供參考如有疑問需要詳細資料請聯(lián)系我們客服人員,齊一生物銷售:021-6034 8496;181214 53965;173021 04490
QY-x1274 人T淋巴瘤轉(zhuǎn)基因細胞Jurkat D,E 【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】
QY-x1275 人T淋巴瘤細胞Jurkat亞系Jurkat77
QY-x1276 人外周血嗜堿性白細胞KU812
QY-x1277 人原髓細胞白血病細胞AL7P/HL-60R
QY-x1278 人急性成髓細胞白血病Kasumi-1
QY-x1279 人T細胞白血病細胞T-CEM
QY-x1280 人EB病毒轉(zhuǎn)化的B細胞CGM1
QY-x1281 人血液白血病細胞TF-1
QY-x1282 人分泌A2抗體B淋巴細胞BB7.2
QY-x1283 人血管平滑肌 T/G
QY-x1284 急性T淋巴細胞白血病細胞TALL-104
QY-x1285 人淋巴細胞瘤細胞JK(Jurkat)
QY-x1286 人T淋巴瘤細胞系Hut-102
QY-x1287 人Burkitt`s淋巴瘤細胞Raji
QY-x1288 人B細胞淋巴瘤細胞系BJAB
QY-x1289 人Burkitts淋巴瘤細胞系CA46
QY-x1290 血細胞瘤細胞系Ramos 【馬β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒廠家】
QY-x1291 人B淋巴細胞瘤細胞RAMOS(RA.1)
QY-x1292 人Burkitt`s淋巴瘤細胞 NAMALWA
QY-x1293 分泌A2抗體B淋巴細胞BB7.2
QY-x1294 轉(zhuǎn)HLA-2基因B細胞T2
QY-x1295 人EBV陽性B淋巴瘤細胞P3HR1
QY-x1296 人組織細胞淋巴瘤細胞U937
QY-x1297 人急性T淋巴母細胞白血病細胞MOLT-4
QY-x1298 人T細胞淋巴瘤Hat-78
QY-x1299 人T細胞淋巴瘤Hurt-78
QY-x1300 人T淋巴細胞系H9
QY-x1301 人T淋巴細胞白血病,CD8+Molt-4
QY-x1302 人B淋巴母細胞HMy2.CIR
QY-x1303 人急性淋巴細胞白血病T淋巴細胞CCRF-CEM
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