1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請?jiān)炷Ｈ〔暮螅瑢?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

QY-x200   HMy2.CIR人B 淋巴母細(xì)胞  CRL-1993                                               

QY-x201   OK負(fù)鼠opossum腎細(xì)胞        CRL-1840                                               

QY-x202   FO骨髓瘤細(xì)胞        CRL-1646                                               

QY-x203   RAW 264.7單核巨噬細(xì)胞白血病  TIB-71                                           

QY-x204   P19畸胎癌細(xì)胞      CRL-1825                                               

QY-x205   C6膠質(zhì)瘤細(xì)胞        CCL-107                                        

QY-x206   RBL-2H3白血病細(xì)胞      CRL-2256                                                【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】

QY-x207   SiHa子宮頸癌細(xì)胞         HTB-35                                         

QY-x208   MG-63骨肉瘤細(xì)胞         CRL-1427                                               

QY-x209   HEC-1-B子宮內(nèi)膜腺癌細(xì)胞  HTB-113                                       

QY-x210   SW1353骨肉瘤細(xì)胞       HTB-94                                         

QY-x211   ES-2卵巢透明細(xì)胞癌細(xì)胞     CRL-1978                                               

QY-x212   Saos-2成骨肉瘤細(xì)胞      HTB-85                                         

QY-x213   MDA-MB-468乳腺癌細(xì)胞      HTB-132                                       

QY-x214   SK-MES-1肺鱗癌細(xì)胞     HTB-58                                         

QY-x215   MDA-MB-435S乳腺癌細(xì)胞    HTB-129                                       

QY-x216   NCI-H661大細(xì)胞肺癌細(xì)胞    HTB-183                                       

QY-x217   ZR-75-1乳腺癌細(xì)胞        CRL-1500                                               

QY-x218   NCI-H292肺癌細(xì)胞淋巴結(jié)轉(zhuǎn)移    CRL-1848                                               

QY-x219   Hs 578T乳腺癌細(xì)胞       HTB-126                                       

QY-x220   U-937組織細(xì)胞淋巴瘤細(xì)胞  CRL-1593.2                                           

QY-x221   CFPAC-1胰腺癌細(xì)胞       CRL-1918                                               

QY-x222   A3淋巴細(xì)胞白血病細(xì)胞        CRL-2570                                                【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】

QY-x223   PANC-1胰腺癌細(xì)胞         CRL-1469                                               

QY-x224   Jurkat,Clone E6-1T 淋巴細(xì)胞白血病細(xì)胞     TIB-152                                        

QY-x225   SK-HEP-1肝癌細(xì)胞          HTB-52                                         

QY-x227   HL-60急性粒細(xì)胞白血病細(xì)胞       CCL-240                                        

QY-x228   HCCLM3肝癌細(xì)胞                                               

QY-x229   TT人甲狀腺癌細(xì)胞        CRL-1803                                               

QY-x230   PLC/PRF/5肝癌亞力山大細(xì)胞        CRL-8024                                               

9020909  Cable, UV curly cord, SMC, QGX                CNY                              【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】

9020908  Hinge, lid, SMC, QGX                 CNY                             

9020643  Waste chute, straight, AS, Cabinet Qsym                  CNY                             

9020632  Kit, hinges (4), Cabinet Qsym            CNY                             

9020629  Door, waste, SP, Cabinet Qsym                  CNY                             

9020625  Kit, repair, Cabinet Qsym                   CNY                             

9020332  EPROM - Motherboard, RGQ           CNY                             

9020233  Foot assembly, rear, CAS           CNY                             

9020158  Insulation disc - chamber bottom, RG3            CNY                             

9020096  Locking Pin, TLyser 2                  CNY                             

9020085  Thrust Washer, TLyser               CNY                             

9239498  V-Housing, LX100              CNY                             

9239490  EX Laser Driver, Uniphase                  CNY                             

9239390  PCR Plate Adapter, M96           CNY                             

9236510  BR 3000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232764  Disposable Troughs, 20 / 30 ml (10)          Troughs holding up to 20 ml of liquid; pack of 10     CNY                             

9236472  BR 9600, Full Agreement         The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY                              【羊階段特異性表面抗原-1(SSEA-1)ELISA試劑盒廠家】

9236443  BR, Installation         The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9239155  Assembly, vertical axis, TWI              CNY                             

9239108  Assy, Sample Needle, Actuator linkage             CNY                             

9232693  MTP-Plate Holder  2.01                   CNY                             

9232629  Bottle, 500-ml, yellow fitting            CNY                             

9232602  Vacuum manifold, plate holder bottomless              CNY                             

9232579  Cooling block, 1,5ml                   CNY                             

9238951  Locking lever, BS15           CNY                             

9238798  Rectangular bucket, sealable with cap             CNY                             

9238794  Angle rotor 6 x 250 ml               CNY                             

9016913  Tubing, press relief valve inlet DISU v2             CNY                             

9238942  Keyboard-Display assembly, BS15             CNY                             

9238753  Screwdriver, hex2mm, z-drive BR8          CNY                             

9238916  Power supply 40W, BS96          CNY