【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x1444       大鼠肝星形細胞CFSC-2G                                     

QY-x1445       大鼠肝星形細胞CFSC-3H & 5H                                   

QY-x1446       大鼠肝上皮樣干細胞WB-F344                                     

QY-x1447       鼠肌原細胞C2C12                                         

QY-x1448       大鼠肺上皮Ⅱ型細胞RLE-6TN                                     

QY-x1449       小鼠肺成纖維細胞L929                                        

QY-x1450       大鼠胸主動脈平滑肌細胞，ATCC：A10VSMC                                   

QY-x1451       小鼠胚成纖維包裝細胞PA317                                      

QY-x1452       小鼠胚成纖維包裝細胞ψ2                                    

QY-x1453       敘利亞倉鼠腎細胞BHK-21                                    

QY-x1454       BHK                                   

QY-x1455       轉(zhuǎn)S9基因倉鼠卵巢細胞ZA1                                        

QY-x1456       轉(zhuǎn)S9基因倉鼠卵巢細胞ZA6                                        

QY-x1457       大鼠巨噬細胞MC                                   

QY-x1458       非洲綠猴腎細胞COS-1                                          【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】

QY-x1459       非洲綠猴腎細胞COS-6                                         

QY-x1460       猴腎細胞系BSC-1                                   

QY-x1461       小鼠腎小球系膜細胞MES-13                                       

QY-x1462       小鼠腎系膜細胞MC53                                   

QY-x1463       大鼠腎成纖微細胞NRK-49F                                         

QY-x1464       小鼠皮膚細胞JB6-C30                                   

QY-x1465       小鼠皮膚細胞JB6-C41                                   

QY-x1466       小鼠前成骨細胞MC-3T3                                       

QY-x1467       小鼠成骨細胞系MC3T3-E1                                   

QY-x1468       鼠胚成骨細胞3T3-E1                                     

QY-x1469       大鼠腎小管上皮細胞，ATCC：CRL-6509NRK                                   

QY-x1470       大鼠腎細胞NRK-52E                                     

QY-x1471       小鼠飼養(yǎng)層上皮細胞HPT-8                                   

QY-x1472       LLC:Leuwis肺癌細胞                                     

QY-x1473       化學轉(zhuǎn)化小鼠鼻咽上皮細胞TMNE                                      

9237329  Upgrade, BR & 3 ext. devices w. sched.      Upgrade BioRobot with three approved integration devices using scheduling software  CNY                            【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】

9237168  Bottle Sheath Supply             CNY                           

9233004  Fan 24V, for BR3000 with VariTerm            CNY                           

9237151  Power Supply        CNY                           

9237123  Upgrade App., QIAquick 96 PCR  On-site application Upgrade for standard QIAGEN QIAquick 96 including kit   CNY                           

9237106  Protocol Dev./Opt., on-site (2-days)     The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed. CNY                           

9237097  Training, BR QIAGEN Advanced  A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.    CNY                           

9236422  Power supply, replacement kit, TWI            CNY                           

9232968  Print YZ96-Adapter-Board Plato-7        CNY                           

9236363  Reservoir XYP              CNY                           

9236353  Tubing Sample Needle to 3-Way Valve         CNY                           

9236329  2-Way Valve (Sheath or vent)        CNY                           

9236160  Potentionmeter, rotary, kit, RapidPlate          CNY                           

9236154  Online Fill Pump RP             CNY                           

9236126  QIAGEN Multiwell-Plate Holder , silver       Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable      CNY                           

9236120  Runner, Drawer, BR8 RD             CNY                           

9235790  Ramp, Locking, Drawer, BR8 RD        CNY                            【人細胞色素P450c21B/21-羥化酶(CYP21B)ELISA試劑盒】

9230745  Z-Lead screw for Plato 7             CNY                           

9235690  Cable X-Main, RCS1            CNY                           

9235572  Shaker Tool, RCS1        CNY                           

9230314  Cable, RS232, syringe pump, 225mm          CNY                           

9230283  Cable, Interface, X-main to Washer             CNY                           

5000-1302      Capacitor 2UF, SKR1            CNY                           

5000-1284      DML 2000 - Power Cord, 220V D Type (GB)            CNY                           

5000-1254      Power Supply PCB, DML2           CNY                           

5000-1250      CPU DML 2000 ASSRY Kit, DML2             CNY                           

6000-3401      RCS Upgrade Package          CNY                           

6000-3116      Plate Washer I - 105 Micron Filters             CNY                           

6000-00183    Plate Washer - Manifold        CNY                           

6000-00177    Plate Washer - Syringe Assembly         CNY                           

5000-1501      Holder Fuse, HTR1        CNY                           

5135-1220      hc2 CT-ID DNA Test            CNY