【小鼠凝血因子Ⅱ(FⅡ)ELISA試劑盒】齊一生物銷售：021－6034 8496；181214 53965；173021 04490

規(guī)格:小規(guī)格48T.大規(guī)格96T

樣本標(biāo)準(zhǔn)：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：酶聯(lián)免疫分析（ELISA）

運(yùn)輸保存：2~8°貯存.保質(zhì)期：6個(gè)月

試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

 

【小鼠凝血因子Ⅱ(FⅡ)ELISA試劑盒】齊一生物主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,檢測(cè)試劑種類齊全,價(jià)格實(shí)惠！購買齊一生物產(chǎn)品的新老客戶都可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),全程提供技術(shù)支持.如需其它Elisa試劑盒說明書及產(chǎn)品價(jià)格,可來電咨詢齊一生物銷售：021-6034 8496；181214 53965；173021 04490

 

試劑盒特點(diǎn)

一、高效、靈敏、特異的抗體；

二、穩(wěn)定的重復(fù)性和可靠性；

三、吸附性能好,空白值低,孔底透明度高的固相載體；

四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；

 

試劑盒可概括四個(gè)方面：

1、免疫酶染色各種細(xì)胞內(nèi)成份的定位.

2、研究抗酶抗體的合成.

3、顯現(xiàn)微量的免疫沉淀反應(yīng).

4、定量檢測(cè)體液中抗原或抗體成份.

【小鼠凝血因子Ⅱ(FⅡ)ELISA試劑盒】ELISA試劑盒為您節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).

elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.

【小鼠凝血因子Ⅱ(FⅡ)ELISA試劑盒】試劑盒制備方法包括以下步驟：

1)抗原表位的計(jì)算機(jī)篩選；

2)抗原的制備；

3)血清的采集；

4)間接ELISA方法的建立；

5)間接ELISA方法的敏感性和特異性驗(yàn)證；

6)試劑盒的穩(wěn)定性驗(yàn)證；

7)對(duì)大量樣本進(jìn)行檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出樣本含量.適用于大批量樣本的檢測(cè)

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【小鼠凝血因子Ⅱ(FⅡ)ELISA試劑盒】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

 

���R ��(j���ng=EN-US>1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
【小鼠的牛血清白蛋白殘留檢測(cè)ELISA試劑盒】注：
1. 用戶在初次使用試劑盒時(shí)，應(yīng)將各種試劑管離心數(shù)分鐘，以便試劑集中到管底。
2. 每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測(cè)量時(shí)先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā)，試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)，酶標(biāo)板加上蓋或覆膜。
4. 未使用完的酶標(biāo)板或者試劑，請(qǐng)于2-8℃保存。標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液、辣根過氧化物酶標(biāo)記親和素工作液請(qǐng)依據(jù)所需的量配置使用。請(qǐng)勿重復(fù)使用已稀釋過的標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液或、辣根過氧化物酶標(biāo)記親和素工作液。
5. 建議檢測(cè)樣品時(shí)均設(shè)雙孔測(cè)定，以保證檢測(cè)結(jié)果的準(zhǔn)確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

 

 

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