【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

齊一生物現(xiàn)貨供應人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購買任意ELISA試劑盒都享受免費代測服務，更多進口原裝品牌試劑盒供應齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)ELISA試劑盒】酶法檢測試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【小鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)ELISA試劑盒】操作注意事項：

1.試劑盒應按標簽說明書儲存，使用前恢復到室溫30分鐘后方可使用。稀稀過后的標準品應丟棄，不可保存。

2.實驗中不用的板條應立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應充分拍干，不要將吸水紙直接放入酶標反應孔中吸水。

7.底物A應揮發(fā)，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應立即讀取OD值。

8.加入試劑的順序應一致，以保證所有反應板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【小鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)ELISA試劑盒】齊一生物客戶寄標本時需注明以下情況：

1、標本編號；2、所測項目；3、是否做復孔；3、聯(lián)系方式；4、實驗后標本是否寄回。

客戶必知：

客戶應對所提供的材料及信息負責，如因客戶提供的材料及信息不準確而引起的實驗延誤或經(jīng)濟損失由客戶承擔。

齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制劑(vaspin)ELISA試劑盒】齊一生物供應，以上資料供參考如有疑問需要詳細資料請聯(lián)系我們客服人員，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9012716Troughs, 25ml (100 troughs + lids)CNY

133230NeXtalStock Sodium cacodyl. pH 6.6 (100)CNY

9002128QIAsymphony RGQ, PrioPLUS"QIAsymphony RGQ Instruments

Installation of QIAsymphony RGQ   instrument & Introductory training 

3 years coverage on parts, labor ａnd travel

On site service, 48 hr response

RGQ, Regional Repair Center service, turn around time 7-10 days

1 Preventive Maintenance service for each year of coverage

Priority service provision over non-contract customers

Documented installation & PM report

Travel, parts, labor ａnd shippment included"CNY

9002048Rotor-Gene Q MDx 6plex, PriorityRotor-Gene Q MDx 6plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

9002043Rotor-Gene Q MDx 6plex SystemCNY

56504QIAamp DNA Investigator Kit (50)CNY

929580QX RNA Size Marker (20µl),KGCNY

929564QX Pro Marker 14-97 kDa (575 µg)CNY

972422PyroMark Q96 SNP APOE (96)For detection of two polymorphism in APOE (APOE 112 ａnd APOE 158)CNY

133224NeXtalStock Sodium ac. pH 4.4 (200)CNY

301425Effectene Transfection Reagent (1 ml)1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well platesCNY

9012693Probe Tubing Holding Screw with bushingCNY

9002008Rotor-Gene Q MDx 2plex, PriorityRotor-Gene Q MDx 2plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

971460therascreen® KRAS Pyro Kit (24) CECNY

292043mericon Turkey (24)CNY

133207NeXtalStock K/Na tartrate (200)CNY

292073mericon Ruminant Kit (24)CNY

929104QIAxcel RNA QC Kit v2.0 (1200)CNY

206042Type-it Fast SNP Probe PCR Kit (100)"Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays 

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"CNY

205314QuantiTect Reverse Transcription (400)CNY

205313QuantiTect Rev.Transcription Kit (200)"For 200 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water. 

The contents of this kit are 4 x the contents of the 205311 (NO bulk!).

Suitable only for cDNA synthesis followed by real-time analyses."CNY

291055mericon GMO Screen CTP2-CP4EPSPS (96)CNY

9001918PyroMark Q96 MD, PrioPLUS"PyroMark Q96 MD Instrument 

Installation of PyroMark Q96 MD instrument & Introductory training 

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"CNY

9001914PyroMark Q24, PrioPLUS"PyroMark Q24 Instrument 

Installation of PyroMark Q24 & Introductory training 

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite or RRC  PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"CNY

9001898QIAxtractor, UV/HEPA (w/o PC) PrioPLUS"QIAxtractor  Instrument 

Installation of QIAxtractor instrument & Introductory training 

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"CNY

133112NeXtalStock Sodium formate (200)CNY

965152QIAamp DNA Blood BioRobot MDx Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates,Buffers,† QIAGEN Protease, ElutionMicrotubes CL, Caps, S-Blocks, Tape PadCNY

962241QIAprep 96 Plus BioRobot Kit (4)CNY

51204FlexiGene DNA Kit (50)For purification of DNA from 50 ml whole blood: Buffers, QIAGEN ProteaseCNY

9015484Reagent Holder, 6-bottle, 30 mlCNY