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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購(gòu)買(mǎi)任意ELISA試劑盒都享受免費(fèi)代測(cè)服務(wù)，更多進(jìn)口原裝品牌試劑盒供應(yīng)齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體(OMgp-Ab)ELISA試劑盒】酶法檢測(cè)試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體(OMgp-Ab)ELISA試劑盒】操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說(shuō)明書(shū)儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開(kāi)蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體(OMgp-Ab)ELISA試劑盒】齊一生物客戶(hù)寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號(hào)；2、所測(cè)項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶(hù)必知：

客戶(hù)應(yīng)對(duì)所提供的材料及信息負(fù)責(zé)，如因客戶(hù)提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶(hù)承擔(dān)。

齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體(OMgp-Ab)ELISA試劑盒】齊一生物供應(yīng)，以上資料供參考如有疑問(wèn)需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

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