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試劑盒產(chǎn)品詳細檢測范圍說明書，隨貨附送。齊一生物銷售：021－6034 8496；181214 53965；173021 04490齊一生物專業(yè)代理多種品牌進口原裝檢測試劑盒。.齊一生物科技有限公司作為酶聯(lián)免疫金牌供應(yīng)商,憑借精湛的技術(shù),全方位的售前售后免費代測技術(shù)服務(wù),共同鑄就高品質(zhì)的ELISA產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,歡迎訂購咨詢服務(wù).

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ELISA試劑盒常遇見問題及解答

【人葡萄糖激酶（GCK）ELISA試劑盒】1。我必須運行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯誤的結(jié)果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運行一個空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計為*佳的性能在給定的體積

【人葡萄糖激酶（GCK）ELISA試劑盒】尊敬的客戶您好！如需技術(shù)支持輔導(dǎo)歡迎撥打齊一生物銷售：021－6034 8496；181214 53965；173021 0449011�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

【人葡萄糖激酶（GCK）ELISA試劑盒】13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。

14。我需要用一個盤子嗎？

可靠的結(jié)果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學(xué)波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯(lián)網(wǎng)在多個科學(xué)數(shù)據(jù)庫訪問。

18。  我光密度稍高（或低）比，是與我的試劑盒說明書。為什么？

試劑盒的光密度的物理條件，如時間和溫度的影響。我們建議您縮短或延長*終孵化與基板解決方案，以補償。

【人葡萄糖激酶（GCK）ELISA試劑盒】19。  高背景的原因是什么？

1）     洗滌不當(dāng)：檢查洗滌液儲量和確保所有建議的洗滌步驟進行。

2）     污染基材：確保沒有污染的金屬離子或氧化劑的基質(zhì)，使用前。在酶聯(lián)免疫吸附底物的開發(fā)時間內(nèi)，保持額外的底物溶液。

3）暴露于光     基材：曝光可能會導(dǎo)致在一個藍色的基板。在黑暗中保存（瓶）的解決方案，直到準(zhǔn)備好進入板。

4）     錯的孵育時間/溫度：一般按照測試協(xié)議對于培養(yǎng)時間和溫度。然而，如果所有的威爾斯是強烈的和同樣顏色的標(biāo)準(zhǔn)稀釋系列中觀察到的強度梯度，那么它可能是必要的觀察到的顏色的底物反應(yīng)，以阻止反應(yīng)更快。

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Service response time (repair): 48 hours/2 business days

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Inspection/maintenance delivery: onsite

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