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重組人胰島素樣生長(zhǎng)因子結(jié)合蛋白-1齊一生物

點(diǎn)擊次數(shù)：493 發(fā)布時(shí)間：2016/9/23
 
 
For research use only 
 
 
 
Recombinant Human IGF-BP1 
 
CATALOG #:         4717-10        10 µg 
            4717-25        25 µg 
            4717-100     100 µg 
            4717-1000       1 mg     
 
LOT #:          _____________________ 
 
SOURCE:        NSO cells 
 
PURITY:          >98% by SDS-PAGE ａnd HPLC analyses 
            Endotoxin level is <0.1 ng per µg of IGF-BP1. 
 
MOL. WEIGHT:      27.5 kDa 
 
FORM:  Sterile filtered ａnd lyophilized with no additives 
 
RECONSTITUTION:   
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. 
The solution can  then be diluted  into other aqueous buffers ａnd store at 4°
C  for 1 week or –
20°
C for future use.   
 
STORAGE CONDITIONS:    
The  lyophilized  IGF-BP1  is best-stored desiccated below 0°
C. Reconstituted IGF-BP1 should 
be stored at working aliquots at -20°
C. 
             
DESCRIPTION:   
Human IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, decidual, and 
kidneys ａnd  is  the most abundant  IGF-BP  in amniotic  fluid. Levels of  IGF-BP1  in serum are 
lowest  after  food.  IGF-BP1  binds  both  IGF-I  and  IGF-II  with  equal  affinity.  Phosphorylated 
IGF-BP1  hinders  IGF  actions,  whereas  nonphosphorylated  IGF-BP1  is  stimulatory.  
Recombinant human  IGF-BP1  is a single polypeptide consisting of 255 amino acid  residues 
with N-terminal His-tag.. 
 
BIOLOGICAL ACTIVITY: 
The ED50  as  determined  by  its  ability  to  inhibit  IGF-I  induced  proliferation  of NUC-1  cells  is 
~100 ng/ml.  
 
 
FOR RESEARCH USE ONLY!  Not to be used on humans. 
 
 
 
         
         
 
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