產(chǎn)品展示

LipoJet™ (Ver. II) DNA In Vitro Transfection Reagen

點(diǎn)擊次數(shù)：27發(fā)布時(shí)間：2016/10/11 16:36:16

更新日期：2023/10/7 10:42:17

所在地：中國(guó)大陸

產(chǎn)品型號(hào)：SL100468

簡(jiǎn)單介紹：利用我們的創(chuàng)新和專有的脂質(zhì)體共軛合成技術(shù)，LipoJet™轉(zhuǎn)染試劑盒，運(yùn)用了新型的含氟陽(yáng)離子脂質(zhì)體配方，同市場(chǎng)上的其他脂質(zhì)體轉(zhuǎn)染試劑有著顯著的區(qū)別，其特點(diǎn)是轉(zhuǎn)染效率高、毒性小。LipoJet™轉(zhuǎn)染試劑盒是*強(qiáng)大的基因轉(zhuǎn)導(dǎo)工具，適于幾乎有所有體外基因轉(zhuǎn)導(dǎo)的應(yīng)用，包括質(zhì)粒DNA、siRNA和shRNA轉(zhuǎn)染和DNA/siRNA共轉(zhuǎn)等。

相關(guān)標(biāo)簽：轉(zhuǎn)染試劑

優(yōu)質(zhì)供應(yīng)

詳細(xì)內(nèi)容

試劑盒內(nèi)容：

- LipoJet™ 試劑，1.0ml，足夠用1000次DNA的轉(zhuǎn)染(24孔板中0.5 ug DNA/孔)足夠用1000次siRNA轉(zhuǎn)染(24孔板中10 nM siRNA/孔)。
- LipoJet™轉(zhuǎn)染緩沖液(5X )，按照化的轉(zhuǎn)染效率配制，8.0ml濃縮液就可以制成40ml的工作液體。
產(chǎn)品特點(diǎn)：
- 適用于廣泛的哺乳動(dòng)物細(xì)胞
- 極高效率地轉(zhuǎn)染DNA，siRNA和DNA/siRNA共轉(zhuǎn)染
- 10 nM siRNA能達(dá)到95%的沉默效應(yīng)
- 無血清和抗生素干擾
- 簡(jiǎn)單易用的操作步驟
- 適用于高通量的應(yīng)用
- 細(xì)胞毒性
儲(chǔ)存條件：
4⁰C儲(chǔ)存。若儲(chǔ)藏合適，產(chǎn)品的穩(wěn)定性能保持12個(gè)月以上。
LipoJet™體外轉(zhuǎn)染試劑具有極廣譜的轉(zhuǎn)染活性。

細(xì)胞類型	DNA轉(zhuǎn)染效率	siRNA轉(zhuǎn)染效率
293, 293T 3T3, NIH 3T3 3T3-L1 A549 B16-F10 BNLCL.2 C2C12 C3H/10T1/2 Caco-2 Rat primary cardiomyocytes CHO COS1 COS7 Human bladder carcinomal cell mES iMEF HBEC HCT116 Hela HepG2 HT-29 HuH-7 LNCaP MC3T3-E1 MCF10A MCF7 MDA-MB-231 MDCK MEF Human primary melanocytes Human primary melanoma Human immortalized microglial mIMCD-3 MRC-5 N2A PC3 RAW 264.7 RPE SH-SY-5Y SK-OV-3 U-2OS VERO	85% - 30% 80% 75% 55% 80% 60% 20% 10% 80% 70% 75% 80% 50% 55% 45% 75% 80% 60% 45% 45% 45% 50% 40% 50% 50% 20% 50% 50% 50% 50% 50% 50% 68% 70% 45% 30% 75% 70% 80% 40%	- 90% at 20 nM - 90% at 20 nM - - 90% at 40 nM - 80 % at 30 nM - - - - - - - - - - - - - - - - - - - 85% at 30 nM 85% at 30 nM - - - - - - - - 90% at 30 nM - - -

以下圖例表明LipoJet™體外轉(zhuǎn)染試劑盒出色的DNA和siRNA轉(zhuǎn)染效率。
LipoJet Transfection Reagent
A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO ａnd HEK293 (left panel) ａnd Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) ａnd antibiotics as recommended by manufacturers. The transfection efficiency were analyzed via FACS 48 hours post transfection.
LipoJet_Cell_Viability
A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) ａnd antibiotics as recommended by manufacturers. The MTT assay (right panel) ａnd phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.
LipoJet_LIPO2000_POLYJET_Comparison_HEK293
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) ａnd PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well ａnd 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) ａnd PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.
LipoJet_LIPO2000_POLYJET_Comparison_HELA
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) ａnd PolyJet™ transfection reagent on Hela cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well ａnd 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) ａnd PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet™試劑介導(dǎo)的DNA/siRNA共轉(zhuǎn)染表現(xiàn)出色的基因沉默效應(yīng)。分別由LipoJet™試劑轉(zhuǎn)染GFP的cDNA (左圖, 0.25µg/孔，24孔板)作為對(duì)照和共轉(zhuǎn)染GFP的cDNA (0.25µg/孔，24孔板)/GFP的siRNA (終濃度10 nM，右圖)至HEK293細(xì)胞 (上圖), Hela細(xì)胞(中圖)和SaoS-2細(xì)胞(下圖)。轉(zhuǎn)染24小時(shí)后，使用尼康Eclipse熒光顯微鏡檢查GFP熒光。結(jié)果顯示，GFP的cDNA和GFP的siRNA共轉(zhuǎn)染導(dǎo)致GFP的表達(dá)被顯著抑制。

LipoJet_HUVEC_mCherry-FITC-siRNA
Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) ａnd FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) ａnd nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection. The pictures were given from Dr. Pan Kong of USC as courtesy.
LipoJet_EG5_HEK293
LipoJet_EG5_HELA
Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) ａnd Hela (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning ａnd bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 ａnd Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 ａnd Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 ａnd Hela cells were visualized 24h post transfection with a Nikon microscope.