產(chǎn)品展示
美國Avidity BirA Biotin-Protein Ligase Kit BirA-500
點(diǎn)擊次數(shù)：119發(fā)布時(shí)間：2016/9/14 15:11:48
更新日期：2023/10/7 10:43:17
所 在 地：中國大陸
產(chǎn)品型號(hào)：BirA-500
相關(guān)標(biāo)簽：美國Avidity
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詳細(xì)內(nèi)容
Enzyme Specifications
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP
intermediate (biotinyl 5’-adenylate) in a highly efficient ａnd targeted manner. The protein is encoded by
the birA gene ａnd is purified using traditional methods. The molecular weight of the BirA biotin-protein
ligase is 33,500 Daltons.
Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin
holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase; biotin-[acetyl-CoA-carboxylase]
ligase; biotin-[acetyl-CoA carboxylase] synthetase; acetyl CoA holocarboxylase synthetase; acetyl CoA
holocarboxylase synthetase; biotin:apocarboxylase ligase; biotin holoenzyme synthetase; HCS.
Source: E. coli
Enzyme Storage buffer: 50mM imidazole (pH 6.8), 50mM NaCl, 10% glycerol, 5mM dithiothreitol (DTT).
Storage conditions: The BirA-500 biotin-protein ligase kit arrives on dry ice (or, in rare cases, ice brix) via
overnight delivery ａnd should be immediately stored at -80°C. After an enzyme vial is thawed it should
be stored at 4°C if it is to be re-used in the near future. For long-term storage keep at -80°C. A vial of
thawed enzyme can be re-frozen by dropping into liquid nitrogen before storing again at -80°C.
The other kit components, BiomixA, BiomixB ａnd BIO-200, can be stored long term at -20°C. For short
term storage 4°C is fine for the BiomixA ａnd BIO-200. The BiomixB should always be stored at -20°C or
colder ａnd thawed immediately before use. There is no problem in thawing ａnd re-freezing any of these
mixtures repeatedly.
Stability: BirA biotin-protein ligase retains >80% of its activity when stored for one month at 4°C. Long
term storage should be at -80°C. Refreezing the enzyme is possible but will reduce its activity upon
thawing again by 10% each time. Therefore we don’t recommend repeated thawing ａnd refreezing
cycles with the BirA.
Concentration: 1.0 mg/mL by A280 (Extinction coefficient; 1.349AU at 280nm λ=1mg/mL BirA).
Purity: >99% by Coomassie staining.
Activity: a minimum of 5,000 Units/μg of BirA
Definition of Activity: One (1) Unit is the amount of enzyme that will biotinylate 1pmol of biotinacceptor
peptide substrate* in 30 minutes at 30°C using the reaction buffers provided ａnd 40μM
peptide substrate final concentration.
* The peptide substrate used in the enzyme assays is a 15-mer variant of sequence #85 identified by P.
Schatz (1).
Contaminating proteases: <0.01% as chymotrypsin-like activity
