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詳細(xì)內(nèi)容
采用Applied Biosystems® TaqMan®基因表達(dá)預(yù)混液，您可以進(jìn)行線性動(dòng)態(tài)范圍大于9 log的精確、可靠的實(shí)時(shí)PCR定量檢測(cè)。TaqMan®基因表達(dá)預(yù)混液是一種經(jīng)過(guò)優(yōu)化的2倍混合物，其中含有進(jìn)行低至一個(gè)拷貝數(shù)敏感度的靶標(biāo)基因檢測(cè)所需的所有成分（模板和引物除外）。其具有擴(kuò)展的實(shí)驗(yàn)穩(wěn)定性，可在自動(dòng)液體處理系統(tǒng)上獲得一致的結(jié)果，因而成為過(guò)夜乃至持續(xù)一周的高通量樣品處理應(yīng)用的理想選擇。
TaqMan®基因表達(dá)預(yù)混液已經(jīng)過(guò)基于TaqMan® 基因表達(dá)Assay和Applied Biosystems®實(shí)時(shí)熒光定量系統(tǒng)的驗(yàn)證，可以為一系列基于基因表達(dá)的實(shí)時(shí)qPCR應(yīng)用提供精確的定量分析，其中包括：
罕見(jiàn)轉(zhuǎn)錄本檢測(cè)
基因表達(dá)分析
病原體檢測(cè)及病毒載量定量
兩種靶標(biāo)共擴(kuò)增
基因家族成員的特異性檢測(cè)
TaqMan® 基因表達(dá)預(yù)混液含有：AmpliTaq Gold® DNA聚合酶UP（超純級(jí)），適于熱啟動(dòng)活化，具有增強(qiáng)的細(xì)菌靶標(biāo)基因的檢測(cè)能力；dNTP和dTTP/dUTP混合物以及尿嘧啶DNA 糖激酶(UDG)，可以限度減少殘余污染；基于ROX染料的被動(dòng)內(nèi)部參照，有助于在Applied Biosystems®實(shí)時(shí)PCR系統(tǒng)上進(jìn)行更為精確定量檢測(cè)。
規(guī)格
| Detection Method: | Primer-Probe Detection |
|---|---|
| Platform: | 7300 System, 7000 System, 7900HT System, StepOnePlus?, Standard Mode, StepOne?, Standard Mode, 7500 System |
| Reaction Speed: | Standard |
| PCR Method: | qPCR |
| Sample Type (General): | DNA |
| Polymerase: | AmpliTaq Gold® DNA Polymerase |
| Product Size: | 1 x 5 mL |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Sample Type (Specific): | cDNA, DNA (Genomic) |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Primer-Probe Compatibility: | TaqMan® |
內(nèi)容及儲(chǔ)存
1 bottle (5 ml) 2X TaqMan® Gene Expression Master Mix sufficient for 200 reactions. Store at 2°C to 8°C.
TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX? Passive Reference, ａnd optimized buffer components.
TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX? Passive Reference, ａnd optimized buffer components.
| 4369016 | TaqMan® Gene Expression Master Mix, 1-Pack (1 x 5 mL) | 500 rxn | 4705 |
| 4369514 | TaqMan® Gene Expression Master Mix, 2-Pack (2 x 5 mL) | 1000 rxn | 8820 |
| 4370048 | TaqMan® Gene Expression Master Mix, Mini-Pack (1 x 1mL) | 100 rxn | 1050 |
| 4440038 | TaqMan® Universal Master Mix II, with UNG 1-Pack (1 x 5 mL) | 200 rxn | 5340 |
| 4440040 | TaqMan® Universal Master Mix II, no UNG 1-Pack (1 x 5 mL) | 200 rxn | 4959 |
| 4440042 | TaqMan® Universal Master Mix II, with UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
| 4440043 | TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
| 4440048 | TaqMan® Universal Master Mix II, no UNG 5-Pack (5 x 5mL) | 2500 rxn | 22050 |
| 4440045 | TaqMan® Universal Master Mix II, with UNG 5-Pack (5 x 5mL) | 2500 rxn | 23360 |
| 4444556 | TaqMan® Fast Advanced Master Mix (1 x 1 mL) | 100 rxn | 1170 |
| 4444557 | TaqMan® Fast Advanced Master Mix (1 × 5 mL) | 500 rxn | 5840 |
| 4444963 | TaqMan® Fast Advanced Master Mix (2 × 5 mL) | 1000 rxn | 11000 |
| 4391128 | TaqMan® PreAmp Master Mix | 40 rxn | 10510 |
| 4488593 | TaqMan® PreAmp Master Mix | 200 rxn | 43220 |
| 4371353 | TaqMan® Genotyping Master Mix, Mini-Pack (1mL) | 100 rxn | 1119 |
| 4371355 | TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL) | 1000 rxn | 8680 |
| 4381656 | TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL) | 2000 rxn | 15760 |
