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技術(shù)文章

HLA-G Antibody (Center) Catalog Number:OM235145

點(diǎn)擊次數(shù):62 發(fā)布時(shí)間:2024/9/30 16:29:16
 

Product Profile

Product Name HLA-G Antibody (Center)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC

 
WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
HLA-6.0
HLAG
Alternative Names
HLA-G
HLA-6.0
HLAG
HLA class I histocompatibility antigen
alpha chain G
HLA G antigen
MHC class I antigen G
More 
Molecular Weight(MW) 38224 Da
Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells
Tissue Specificity Expressed in trophoblasts.
Cellular Localization Membrane; Single-pass type I membrane protein

Database Links

Entrez Gene 3135

Application

  • Application

    Western blot analysis of HLA-G Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.

  • Application

    Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.

  • Application

    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    HLA-G Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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