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技術(shù)文章

HLA-G antibody Catalog Number：OM257160

點(diǎn)擊次數(shù)：63 發(fā)布時(shí)間：2024/9/30 16:28:29
 

Product Profile

Product Name HLA-G antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal [MEM - G / 9] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail. [provided by RefSeq]
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Immunogen 
Recombinant full length protein (Human).

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number MEM-G/9
Host Species Mouse
Tested Applications

ELISAFACSIHC-FWB
 
ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity

HumanPrimate
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
HLA G
HLAG
MHC-G
MHC G
MHCG
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Target HLA-G
Tissue Specificity Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G ａnd HLA-G1 complementary DNA.This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G.
Cellular Localization Cell Membrane

Application

  • Application

    Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC ( ) ａnd anti-human HLA-G (MEM-G/11) FITC .

  • Application

    Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) ａnd protein G. HLA-G was detected by anti-human HLA-G (4H84) ａnd goat anti-mouse HRP in cell lysate (Lane 1) ａnd in the immunoprecipitate (Lane 2).

Application Notes
ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot ａnd store at -20°C or below. Avoid freeze-thaw cycles.
Storage Buffer Phosphate buffered saline pH 7.4 containing 15mM sodium azide

原創(chuàng)作者：上海篤瑪生物科技有限公司

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