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技術(shù)文章

HLA-G antibody Catalog Number：OM257151

點(diǎn)擊次數(shù)：68 發(fā)布時(shí)間：2024/9/30 16:26:35
 

Product Profile

Product Name HLA-G antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal [MEM - G / 1] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail. [provided by RefSeq]
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Immunogen 
Recombinant full length denatured heavy chain (Human).

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number MEM-G/1
Host Species Mouse
Tested Applications

IHCIHC-PWB
 
IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.:
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Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
HLA G
HLAG
MHC-G
MHC G
MHCG
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Target HLA-G
Tissue Specificity The antibody reacts with denaturated HLA-G heavy chain.
Cellular Localization Cell Membrane

Application

  • Application

    Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) . Fig. 1B - first-trimester placenta (paraffin-embedded sections)

  • Application

    The antibody stains infiltrating macrophages in pulmonary diseases. In the left corner see detail of macropage.

Application Notes
IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.:
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Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer Phosphate buffered saline pH 7.4 containing 15mM sodium azide

原創(chuàng)作者：上海篤瑪生物科技有限公司

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