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技術(shù)文章

HLA-G antibody Catalog Number：OM257159

點(diǎn)擊次數(shù)：63 發(fā)布時(shí)間：2024/9/30 16:19:34
 

Product Profile

Product Name HLA-G antibody
Antibody Type Primary Antibodies
Product description 
Monoclonal Antibody to HLA-G (Human) Azide freeHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail. [provided by RefSeq]
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Immunogen 
Recombinant human HLA-G refolded with beta2-microglobulin ａnd peptide.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number MEM-G/9
Host Species Mouse
Tested Applications

ELISAFACSIHC-FIPWB
 
FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml):
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
142871
HLAG
P17693
MHC-G
MHCG
3135
HLA-G
HLA G
MHC G
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Target HLA-G
Tissue Specificity The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

Application

  • Application

    Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC ａnd anti-human HLA-G (MEM-G/11) FITC.

  • Application

    Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) ａnd protein G. HLA-G was detected by anti-human HLA-G ａnd goat anti-mouse HRP in cell lysate (Lane 1) ａnd in the immunoprecipitate (Lane 2).

Application Notes
FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml):

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4

原創(chuàng)作者：上海篤瑪生物科技有限公司

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