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技術文章

SUMO2/3 Antibody (N-term M1) Catalog Number：OM222303

點擊次數：95 發(fā)布時間：2024/9/30 16:13:49
 

Product Profile

Product Name SUMO2/3 Antibody (N-term M1)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB
 
WB~~1:50~100:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
SMT3A
SMT3H2
Alternative Names
SUMO2
SMT3A
SMT3H2
Small ubiquitin-related modifier 2
HSMT3
SMT3 homolog 2
SUMO-3
Sentrin-2
Ubiquitin-like protein SMT3A
More 
Molecular Weight(MW) 10871 Da
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 ａnd linkage to the E2 enzyme UBE2I, ａnd can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication ａnd repair, mitosis ａnd signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins
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Tissue Specificity Broadly expressed.
Cellular Localization Nucleus. Note=Nuclear bodies.

Database Links

Entrez Gene 6613

Application

  • Application

    Western blot analysis of SUMO2/3 Antibody (N-term M1) in Jurkat cell line lysates (35ug/lane). SUMO2/3 (arrow) was detected using the purified Pab.

  • Application

    Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (N-term M1) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.

Application Notes
WB~~1:50~100:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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