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技術(shù)文章

SUMO2/3 Antibody (N-term) Catalog Number:OM222304

點(diǎn)擊次數(shù):65 發(fā)布時間:2024/9/30 16:12:33
 

Product Profile

Product Name SUMO2/3 Antibody (N-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB

 
WB~~1:100~500:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
SMT3A
SMT3H2
Alternative Names
SUMO2
SMT3A
SMT3H2
Small ubiquitin-related modifier 2
HSMT3
SMT3 homolog 2
SUMO-3
Sentrin-2
Ubiquitin-like protein SMT3A
More 
Molecular Weight(MW) 10871 Da
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins
More 
Tissue Specificity Broadly expressed.
Cellular Localization Nucleus. Note=Nuclear bodies.

Database Links

Entrez Gene 6613

Application

  • Application

    Western blot analysis of anti-SUMO2/3 N-term polyclonal antibody in 293 cell lysate. Sumo2/3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  • Application

    Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (M1) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.

Application Notes
WB~~1:100~500:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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