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技術(shù)文章

SUMO2/3 Antibody Catalog Number：OM292543

點擊次數(shù)：49 發(fā)布時間：2024/9/30 16:06:20
 

Product Profile

Product Name SUMO2/3 Antibody
Antibody Type Primary Antibodies
Product description 
SUMO2/3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis ａnd protein stability. All SUMO proteins localize to the nucleus ａnd are covalently conjugated, affecting protein structure, function ａnd interactions. SUMO2 ａnd 3 are 96% identical ａnd are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 ａnd SUMO2 ａnd 3 remain to be identified.1) Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
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Immunogen 
SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2. The immunogen is located within the last 50 amino acids of SUMO2/3.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAIFIHC-PWB
 
SUMO2/3 antibody can be used for detection of SUMO2 ａnd SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SUMO2
Alternative Names
SUMO2/3 Antibody: HSMT3
SMT3B
SUMO3
Smt3A
SMT3H2
SMT3A
Small ubiquitin-related modifier 2
HSMT3
SUMO-2
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Molecular Weight(MW) Predicted: 15 kDa Observed: 15 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (93%), Pig: (93%)

Database Links

Entrez Gene 6613
Protein Accession AAH71645

Application

  • Application

    Western blot analysis of SUMO2/3 in Jurkat cell lysate with SUMO2/3 antibody at (A) 1 ａnd (B) 2 μg/mL.

  • Application

    Immunofluorescence of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 20 μg/mL.

  • Application

    Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
SUMO2/3 antibody can be used for detection of SUMO2 ａnd SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:

Additional Information

Form Liquid
Storage Instructions SUMO2/3 antibody can be stored at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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