企業(yè)檔案

Product Name	B-Myb Antibody (C-20)
Antibody Type	Primary Antibodies
Modification Notes	The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb ａnd v-Ets which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy terminal domain of c-Myb results in enhanced transactivating capacity ａnd in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells ａnd its expression decreases as cells differentiate. A second member of the Myb proto-oncogene family, B-Myb, encodes a second sequence-specific DNA binding protein. B-Myb RNA levels are low or undetectable in quiescent cells but increase at the G1/S-phase transition following mitogenic stimulation. Studies suggest that B-Myb expression rescues cells from p53-induced G1 arrest mediated by p21.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFELISAGSChIP
	recommended for detection of B-Myb of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine, porcine ａnd avian:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Alternative Names	epie mapping at the C-terminus of B-Myb of human origin
Tissue Specificity	epie mapping at the C-terminus of B-Myb of human origin

Entrez Gene	4605

Application

Antibodies tested include p-B-Myb (H-3): (A,B,C) ａnd B-Myb (C-20): (D,E,F).
Application

Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear ａnd cylasmic localization.
Application

Western blot analysis of B-Myb expression in non-transfected: (A) ａnd mouse B-Myb transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of B-Myb expression in non-transfected: (A) ａnd human B-Myb transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of B-Myb expression in HeLa whole cell lysate.

Application Notes	recommended for detection of B-Myb of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine, porcine ａnd avian:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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