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技術(shù)文章

c-Myb Antibody (H-141) Catalog Number：OM167077

點(diǎn)擊次數(shù)：33 發(fā)布時間：2024/9/30 15:46:31
 

Product Profile

Product Name c-Myb Antibody (H-141)
Antibody Type Primary Antibodies
Modification Notes The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb ａnd v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy terminal domain of c-Myb results in enhanced transactivating capacity ａnd in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells ａnd its expression decreases as cells differentiate. A second member of the Myb proto-oncogene family, B-Myb, encodes a second sequence-specific DNA binding protein. B-Myb RNA levels are low or undetectable in quiescent cells but increase at the G1 to S phase transition following mitogenic stimulation. Studies suggest that B-Myb expression rescues cells from p53-induced G1 arrest mediated by p21.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFELISAGSChIP
 
recommended for detection of c-Myb of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine ａnd porcine:
Species Reactivity

HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epie corresponding to amino acids 500-640 mapping at the C-terminus of c-Myb of human origin
Tissue Specificity epie corresponding to amino acids 500-640 mapping at the C-terminus of c-Myb of human origin

Database Links

Entrez Gene 4602

Application

  • Application

    Western blot analysis of c-Myb expression in Jurkat whole cell lysate.

  • Application

    Western blot analysis of c-Myb expression in HL-60 whole cell lysate.

Application Notes
recommended for detection of c-Myb of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine ａnd porcine:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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